Omics Core

摘要：OMICS Core（Core B）的主要功能是为路易斯维尔大学的成员提供 肝脏生物学和毒理学COBRE拥有代谢组学所需的专业知识、资源和设施， 对收集的生物样本进行蛋白质组学和生物信息学分析，以研究肝损伤、营养、肠道：肝脏 相互作用和肝脏：环境/毒物/药物相互作用。核心B人员将协助设计 样品收集协议，将推动开发和执行新的和强大的色谱 样品分离方法和用于分析蛋白质、肽和 代谢物，目的是更好地了解分子机制的演变， 与肝功能障碍相关或参与肝功能障碍。核心B将确保样品的均匀性和标准化 准备、方法开发、仪器性能、数据收集、数据报告和数据共享。 核心B努力为COBRE的整体成功和效用做出贡献，并增强基础设施 以及整个机构的技术和学术能力。除了作为服务的功能外， 核心B还将促进COBRE成员的教育和培训， 研究人员关于国家的最先进的组学技术;特别注意COBRE少年 investigators.此外，核心B将成为一个独立和自我支持的实体， 已经实施并在我们的阶段中取得成功的综合方法 我眼镜蛇。 在肝脏生物学和毒理学COBRE II期期间，核心B的目标将由 具体目标： 1)支持当前的COBRE和UofL健康科学中心（HSC）项目。 2)教育和培训学员和其他调查人员。 3)开发新的治疗方案和技术。 4)成为COBRE和UofL HSC的自我维持功能。

Abstract: The primary function of OMICS Core (Core B) is to provide members of the University of Louisville Hepatobiology and Toxicology COBRE with the expertise, resources, and facilities needed for metabolomic, proteomic, and bioinformatic analyses of biological samples collected to study liver injury, nutrition, gut:liver interactions, and liver:environment/toxicant/drug interactions. Core B personnel will assist with the design of sample collection protocols, will drive the development and the execution of novel and robust chromatographic methods for sample separation and mass spectrometric methods for the analysis of proteins, peptides, and metabolites with a goal of providing a greater understanding of the evolution of molecular mechanisms that correlate with or participate in liver dysfunction. Core B will ensure uniformity and standardization in sample preparation, method development, instrument performance, data collection, data reporting, and data sharing. Core B endeavors to contribute to the overall success and utility of the COBRE and to enhance the infrastructure and the technical and academic capabilities of the institution as a whole. In addition to its function as a service core, Core B will also promote the education and training of the COBRE members and other biomedical investigators with regards to the state-of-the-art omics technologies; with particular attention to the COBRE junior investigators. Furthermore, Core B will become an independent and self-supporting entity using a set comprehensive approaches that have been already implemented and demonstrated their success in our Phase I COBRE. During the Hepatobiology and Toxicology COBRE Phase II period the goals of Core B will be addressed by the following Specific Aims: 1) To support current COBRE and UofL Health Science Center (HSC) projects. 2) To educate and train mentees and other investigators. 3) To develop new protocols and techniques. 4) To become self-sustaining feature of the COBRE and UofL HSC.