Ultra Performance Liquid Chromatography High Resolution High Mass Accuracy Mass Spectrometer

超高效液相色谱高分辨率高质量精度质谱仪

• 批准号: 8826389
• 负责人: Xiang Zhang
• 金额: $ 60万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-02-27 至 2016-02-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826389
• 关键词: Basic Science; Biomedical Research; Clinical Research; Communities; Complement; Coupled; Education; Fourier transform ion cyclotron resonance; Funding; High Pressure Liquid Chromatography; Hybrids; Institutes; Ions; Liquid Chromatography; Maintenance; Manufacturer Name; Mass Spectrum Analysis; Metabolic Pathway; Performance; Preventive; Request for Proposals; Research; Resolution; Risk; Sampling; Time; Translational Research; Universities; base; career development; instrument; mass spectrometer; metabolomics; operation; programs; prospective; two-dimensional

 DESCRIPTION (provided by applicant): This proposal requests funds for acquisition and continuing operation of a two-dimensional ultra performance liquid chromatography coupled with a high resolution and high accuracy mass spectrometer with tandem MS/MS capability (2DLC-MS/MS) for metabolomics. This instrument will be located in the Shumaker Research Building (room 335) at the Center for Regulatory Environmental and Analytical Metabolomics (CREAM), as a part of integrated metabolomics platforms to support the basic, translational and clinical research at the University of Louisville. The proposed instrument will complement and gradually replace our use of the existing hybrid quadrupole linear ion-trap and Fourier transform ion cyclotron resonance mass spectrometry (LTQ- FTICR-MS) instrument. This LTQ-FTICR-MS has been formally designated as in "retired status" by the manufacturer as of January 2014 and the manufacturer will now only provide preventive maintenance. We are under risk, at any moment, of losing the capability of conducting high resolution high accuracy mass spectrometry-based metabolomics analyses at the CREAM Center. The Specific Aims of this proposal are provision of the following for the University of Louisville biomedical research community: Specific Aim 1: For the established Major Users (78% allotment of time): Provision of increased capacity for identification and quantification of metabolites with complementary analyses of spatial and temporal flux through metabolic pathways. Specific Aim 2: For appropriate Research Centers & Institutes, less frequent (<100 samples / year) Users, and prospective Users of mass spectrometry (17% allotment of time): Vigorous promotion of the use of high accuracy and high resolution mass spectrometry in biomedical research with an education program for research and scientific career development.

 描述（由申请人提供）：本提案要求提供资金，用于购买和继续运行二维超高效液相色谱仪，以及具有串联MS/MS能力（2DLC-MS/MS）的高分辨率和高准确度质谱仪，用于代谢组学。该仪器将位于监管环境和分析代谢组学中心（CREAM）的Shumaker研究楼（335室），作为支持路易斯维尔大学基础、转化和临床研究的综合代谢组学平台的一部分。拟议的仪器将补充并逐步取代我们使用现有的混合四极线性离子阱和傅里叶变换离子回旋共振质谱（LTQ-FTICR-MS）仪器。该LTQ-FTICR-MS已于2014年1月被制造商正式指定为“退役状态”，制造商现在仅提供预防性维护。我们随时都有可能失去在CREAM中心进行高分辨率高精度质谱代谢组学分析的能力。该提案的具体目标是为路易斯维尔大学生物医学研究界提供以下内容：具体目标1：针对已建立的主要用户（78%的时间分配）：提供更高的代谢物鉴定和定量能力，并通过代谢途径对空间和时间通量进行补充分析。具体目标二：对于适当的研究中心和研究所，不太频繁（<100个样品/年）的用户和质谱的潜在用户（17%的时间分配）：大力推广高精度和高分辨率质谱在生物医学研究中的使用，并提供研究和科学职业发展的教育计划。

项目成果

Using Multiple Analytical Platforms to Investigate the Androgen Depletion Effects on Fecal Metabolites in a Mouse Model of Systemic Lupus Erythematosus.

使用多个分析平台研究雄激素消耗对系统性红斑狼疮小鼠模型粪便代谢物的影响。

• DOI: 10.1021/acs.jproteome.9b00558
• 发表时间: 2020
• 期刊: Journal of proteome research
• 影响因子: 4.4
• 作者: Yuan,Fang;Harder,James;Ma,Jing;Yin,Xinmin;Zhang,Xiang;Kosiewicz,MicheleM
• 通讯作者: Kosiewicz,MicheleM

Cell cycle induction in human cardiomyocytes is dependent on biosynthetic pathway activation.

• DOI: 10.1016/j.redox.2021.102094
• 发表时间: 2021-10
• 期刊: Redox biology
• 影响因子: 11.4
• 作者: Abouleisa RRE;McNally L;Salama ABM;Hammad SK;Ou Q;Wells C;Lorkiewicz PK;Bolli R;Mohamed TMA;Hill BG
• 通讯作者: Hill BG