Ultra Performance Liquid Chromatography High Resolution High Mass Accuracy Mass Spectrometer

 描述(由申请人提供)：本提案要求资金用于购买和继续运行用于代谢组学的二维超高效液相色谱仪和具有串联MS/MS能力的高分辨率和高精度质谱仪(2DLC-MS/MS)。该仪器将设在监管环境和分析代谢组学中心(CREAM)的舒梅克研究大楼(335室)，作为支持路易斯维尔大学基础、翻译和临床研究的综合代谢组学平台的一部分。建议的仪器将补充和逐步取代我们现有的四极线性离子陷阱和傅里叶变换离子回旋共振质谱仪(LTQ-FTICR-MS)的使用。自2014年1月起，该LTQ-FTICR-MS已被制造商正式指定为“退役状态”，制造商现在只提供预防性维护。我们随时都有可能失去在CREAM中心进行基于高分辨率、高精度质谱学的代谢组学分析的能力。这项提议的具体目标是为路易斯维尔大学生物医学研究界提供以下内容：具体目标1：针对既定的主要用户(78%的时间分配)：通过对代谢途径的空间和时间流量进行补充分析，提高识别和量化代谢物的能力。具体目标2：针对适当的研究中心和研究所、不太频繁(每年100个样本)的用户和潜在的质谱学用户(17%的时间分配)：通过研究和科学事业发展的教育计划，大力推广高精度和高分辨率质谱学在生物医学研究中的使用。

 DESCRIPTION (provided by applicant): This proposal requests funds for acquisition and continuing operation of a two-dimensional ultra performance liquid chromatography coupled with a high resolution and high accuracy mass spectrometer with tandem MS/MS capability (2DLC-MS/MS) for metabolomics. This instrument will be located in the Shumaker Research Building (room 335) at the Center for Regulatory Environmental and Analytical Metabolomics (CREAM), as a part of integrated metabolomics platforms to support the basic, translational and clinical research at the University of Louisville. The proposed instrument will complement and gradually replace our use of the existing hybrid quadrupole linear ion-trap and Fourier transform ion cyclotron resonance mass spectrometry (LTQ- FTICR-MS) instrument. This LTQ-FTICR-MS has been formally designated as in "retired status" by the manufacturer as of January 2014 and the manufacturer will now only provide preventive maintenance. We are under risk, at any moment, of losing the capability of conducting high resolution high accuracy mass spectrometry-based metabolomics analyses at the CREAM Center. The Specific Aims of this proposal are provision of the following for the University of Louisville biomedical research community: Specific Aim 1: For the established Major Users (78% allotment of time): Provision of increased capacity for identification and quantification of metabolites with complementary analyses of spatial and temporal flux through metabolic pathways. Specific Aim 2: For appropriate Research Centers & Institutes, less frequent (<100 samples / year) Users, and prospective Users of mass spectrometry (17% allotment of time): Vigorous promotion of the use of high accuracy and high resolution mass spectrometry in biomedical research with an education program for research and scientific career development.