OMICS CORE

组学核心

• 批准号: 8813877
• 负责人: Xiang Zhang
• 金额: $ 34.63万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-10 至 2021-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8813877
• 关键词: Ablation; Acetylation; Affinity; Alcohols; Arsenic; Binding; Bioinformatics; Biological; Blood; Centers of Research Excellence; Chemicals; Complex Mixtures; Curcumin; Cysteine; DNA Binding Domain; Diet; Drug Interactions; Environment; Ethanol; Event; Feces; Gas Chromatography; Gel; Goals; Hepatic; Hepatic Stellate Cell; High Fat Diet; Immunoprecipitation; Inductively Coupled Plasma Mass Spectrometry; Inflammation; Injury; Intestines; Iodoacetamide; Iodoacetic Acid; Ions; Label; Lactobacillus casei rhamnosus; Large Intestine; Lasers; Linoleic Acids; Liquid Chromatography; Liver; Liver diseases; Mass Spectrum Analysis; Measurement; Measures; Mediating; Metals; Methods; Methylation; Mus; Pathology; Phenotype; Phosphopeptides; Phosphorylation; Plasma; Post-Translational Protein Processing; Production; Protein Fragment; Protein Isoforms; Proteins; Proteomics; Recombinant Proteins; Regulation; Request for Proposals; Research; Resolution; Role; Sampling; Science; Site; Small Intestines; Stable Isotope Labeling; Steatohepatitis; Techniques; Technology; Time; Tissue Sample; Tissues; Toxicology; Toxin; Unsaturated Fatty Acids; Urine; Volatile Fatty Acids; Zinc; exosome; feeding; liquid chromatography mass spectrometry; liver inflammation; liver injury; mass spectrometer; meetings; metabolomics; non-alcoholic fatty liver; novel; nutrition; oxidation; phosphodiesterase IV; protein complex; protein metabolite; recombinant peptide; research study; response; transcription factor; tumor growth; two-dimensional; zinc-binding protein

High-throughput 'omics analysis platforms are the technologies of choice for analyzing complex mixtures derived from tissues and biological matrices such as liver, feces, intestine, blood and urine. To maximize our capability for deciphering the mechanism and therapy for liver injury, nutrition and gut:liver interactions, and liver:environment/toxin/drug interactions, we propose an OMICS Core in this Hepatobiology and Toxicology COBRE proposal. Our OMICS Core is composed of three major components including Metabolomics Core, Proteomics Core, and Bioinformatics. The overarching goal of the OMICS Core is to support the proposed COBRE research. This goal will be met through application of state of the art separations science and mass spectrometry techniques to analyze research samples containing complex mixtures of metabolites, proteins and protein fragments. This goal is achieved through the following aims: Specific Aim 1: Application of liquid chromatography mass spectrometry (LC-MS), comprehensive two- dimensional gas chromatography time-of-flight mass spectrometry (GCxGC/TOF-MS) and integrated laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) platforms for quantitative analysis of metabolites, metal ions, and metal-protein complex in biological samples. Specific Aim 2: Application of high-resolution multidimensional liquid chromatography mass spectrometry (MDLC-MS) and affinity enrichment methods for targeted protein quantification.

高通量组学分析平台是分析复杂混合物的首选技术 来源于组织和生物基质，例如肝脏、粪便、肠、血液和尿液。最大化我们 能够破译肝损伤，营养和肠道的机制和治疗：肝脏相互作用，以及 肝脏：环境/毒素/药物相互作用，我们提出了一个OMICS核心在这个肝生物学和毒理学 COBRE提案。我们的OMICS核心由三个主要组成部分组成，包括代谢组学核心， 蛋白质组学核心和生物信息学。OMICS核心的首要目标是支持拟议的 COBRE研究。这一目标将通过应用最先进的分离科学和质量来实现。 光谱分析技术，用于分析含有代谢物、蛋白质、 和蛋白质片段。这一目标是通过以下目标实现的： 具体目标1：液相色谱-质谱联用技术（LC-MS）的应用，综合两个- 三维气相色谱飞行时间质谱（GCxGC/TOF-MS）和集成激光 消融电感耦合等离子体质谱（LA-ICP-MS）平台，用于定量分析 生物样品中的代谢物、金属离子和金属-蛋白质复合物。 具体目标2：高分辨率多维液相色谱质谱的应用 （MDLC-MS）和亲和富集方法用于靶向蛋白质定量。