Omics Core

组学核心

• 批准号: 8978011
• 负责人: Xiang Zhang
• 金额: $ 46.09万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-15 至 2021-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8978011
• 关键词: Acids; Acrolein; Affinity; Alcohol Nutrition; Alcoholic Liver Diseases; Alcohols; Animals; Attention; Attenuated; Automobile Driving; Bioinformatics; Biological; Biotin; Blood; Blood specimen; Body Weight; Cell Line; Cells; Chronic; Complex Mixtures; Curcumin; Data; Data Collection; Data Reporting; Data Set; Development; Drug Interactions; Embryo; Endotoxemia; Ensure; Environment; Epigenetic Process; Extracellular Matrix; Feces; Fibroblasts; Gas Chromatography; Ginger; Goals; Grapefruit; Hepatic; High Pressure Liquid Chromatography; Histone Acetylation; Histones; Human Resources; Inflammation; Injury; Institution; Interleukin-2; Intestines; Lactobacillus casei rhamnosus; Liquid substance; Liver; Liver Dysfunction; Liver diseases; Lung; Lysine; Mass Spectrum Analysis; Measures; Mediating; Metabolic; Methodology; Methods; Molecular; Molecular Evolution; Mus; Neural Crest Cell; Organ; Pattern; Peptides; Performance; Post-Translational Protein Processing; Preparation; Protein Analysis; Protein Fragment; Proteins; Proteomics; Protocols documentation; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Role; Sampling; Science; Serum; Services; Standardization; Sulforaphane; TNFRSF5 gene; Techniques; Technology; Time; Tissue Sample; Tissues; Training and Education; Transcriptional Regulation; Unsaturated Fats; Urine; abstracting; adduct; alcohol effect; data sharing; design; exosome; histone methylation; histone modification; instrument; liquid chromatography mass spectrometry; liver injury; long chain fatty acid; male; meetings; member; metabolomics; method development; multiple reaction monitoring; nanoparticle; novel; nutrition; phosphoproteomics; programs; protein metabolite; response; sample collection; saturated fat; success; toxicant; tributyrin; two-dimensional

Abstract: The primary function of Omics Core is to provide members of the ULARC with the expertise, resources and facilities needed for metabolomic, proteomic and bioinformatic analyses of biological samples collected to study liver injury, nutrition, gut:liver interactions, and liver:environment/toxicant/drug interactions. OMICS Core personnel will assist with the design of sample collection protocols, will drive the development and the execution of novel and robust chromatographic methods for sample separation and mass spectrometric methods for the analysis of proteins, peptides, and metabolites with a goal of providing a greater understanding of the evolution of molecular mechanisms that correlate with or participate in liver dysfunction. The core will ensure uniformity and standardization in sample preparation, method development, instrument performance, data collection, data reporting and data sharing. The Core endeavors to contribute to the overall success and utility of the ULARC and to enhance the infrastructure and the technical and academic capabilities of the institution as a whole. In addition to its function as a service core, we will also promote the education and training of the ULARC members with regards to state-of–the-art Omics and Bioinformatics methods; with particular attention to the ULARC junior investigators.

抽象的： Omics Core 的主要功能是为 ULARC 成员提供专业知识、资源和 对收集的生物样本进行代谢组学、蛋白质组学和生物信息学分析所需的设施 研究肝损伤、营养、肠道：肝脏相互作用以及肝脏：环境/毒物/药物相互作用。组学核心 人员将协助设计样本采集方案，推动开发和 执行用于样品分离和质谱分析的新颖且稳健的色谱方法 蛋白质、肽和代谢物的分析方法，旨在提供更好的分析结果 了解与肝功能障碍相关或参与肝功能障碍的分子机制的演变。 核心将确保样品制备、方法开发、仪器的一致性和标准化 性能、数据收集、数据报告和数据共享。核心致力于为整体做出贡献 ULARC 的成功和实用性，并增强基础设施以及技术和学术能力 该机构作为一个整体。除了作为服务核心的功能外，我们还将推动教育和 对 ULARC 成员进行最先进的组学和生物信息学方法方面的培训；和 特别关注 ULARC 初级调查员。