Omics Core

组学核心

• 批准号: 10608169
• 负责人: Xiang Zhang
• 金额: $ 29.85万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-10 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10608169
• 关键词: Address; Applications Grants; Attention; Award; Bioinformatics; Biological; Biological Assay; Centers of Research Excellence; Chemicals; Chromatography; Clinical Research; Communities; Complex Mixtures; Continuing Education; Data; Data Analyses; Data Collection; Data Reporting; Derivation procedure; Development; Dimensions; Disease; Drug Interactions; Education; Ensure; Environment; Equipment; Fee-for-Service Plans; Funding; Future; Goals; Health Sciences; Hepatology; Human Resources; Individual; Infrastructure; Institution; Intervention Studies; Leadership; Liver; Liver Dysfunction; Liver diseases; Malignant neoplasm of liver; Mass Spectrum Analysis; Methods; Modality; Modeling; Modification; Molecular Evolution; Peptides; Performance; Phase; Preparation; Privatization; Procedures; Protein Analysis; Protein Fragment; Proteins; Proteomics; Protocols documentation; RNA; Reagent; Research; Research Activity; Research Personnel; Research Support; Resources; S10 grant; Sampling; Science; Services; Standardization; Statistical Data Interpretation; Techniques; Technology; Toxicology; Training; Training and Education; Translational Research; United States National Institutes of Health; Universities; career; career development; clinical infrastructure; data sharing; design; epitranscriptomics; experimental study; fatty liver disease; innovation; instrument; liver development; liver injury; meetings; member; metabolomics; method development; molecular marker; non-alcoholic; novel; nutrition; operation; problem drinker; programs; sample collection; small molecule; success; theories; tool; toxicant

Abstract: The primary function of OMICS Core (Core B) is to provide members of the University of Louisville Hepatobiology and Toxicology COBRE with the expertise, resources, and facilities needed for metabolomic, proteomic, and bioinformatic analyses of biological samples collected to study liver injury, nutrition, gut:liver interactions, and liver:environment/toxicant/drug interactions. Core B personnel will assist with the design of sample collection protocols, will drive the development and the execution of novel and robust chromatographic methods for sample separation and mass spectrometric methods for the analysis of proteins, peptides, and metabolites with a goal of providing a greater understanding of the evolution of molecular mechanisms that correlate with or participate in liver dysfunction. Core B will ensure uniformity and standardization in sample preparation, method development, instrument performance, data collection, data reporting, and data sharing. Core B endeavors to contribute to the overall success and utility of the COBRE and to enhance the infrastructure and the technical and academic capabilities of the institution as a whole. In addition to its function as a service core, Core B will also promote the education and training of the COBRE members and other biomedical investigators with regards to the state-of-the-art omics technologies; with particular attention to the COBRE junior investigators. Furthermore, Core B will become an independent and self-supporting entity using a set comprehensive approaches that have been already implemented and demonstrated their success in our Phase I COBRE. During the Hepatobiology and Toxicology COBRE Phase II period the goals of Core B will be addressed by the following Specific Aims: 1) To support current COBRE and UofL Health Science Center (HSC) projects. 2) To educate and train mentees and other investigators. 3) To develop new protocols and techniques. 4) To become self-sustaining feature of the COBRE and UofL HSC.

摘要：OMICS核心(核心B)的主要功能是为路易斯维尔大学的成员提供 肝生物学和毒理学科布雷拥有代谢组学所需的专业知识、资源和设施， 为研究肝损伤、营养、肠道：肝脏而收集的生物样本的蛋白质组学和生物信息学分析 相互作用和肝脏：环境/毒物/药物的相互作用。核心B人员将协助设计 样品收集协议，将推动新的和强大的色谱学的开发和执行 蛋白质、多肽和蛋白质的样品分离和质谱分析方法 代谢物，目的是更好地理解 与肝功能障碍相关或参与肝功能障碍。核心B将确保样品的一致性和标准化 准备、方法开发、仪器性能、数据收集、数据报告和数据共享。 核心B致力于为科布雷的整体成功和效用做出贡献，并加强基础设施 以及整个机构的技术和学术能力。除了作为一项服务的功能外 CORE、CORE B还将促进科布雷成员和其他生物医学人员的教育和培训 与最先进的组学技术有关的调查人员；特别关注科布雷少年 调查人员。此外，核心B将成为一个独立和自给自足的实体，使用一套 已经实施的综合方法，并在本阶段展示了它们的成功 我是科布雷。 在肝脏生物学和毒理学科布雷第二阶段期间，核心B的目标将通过 以下是具体目标： 1)支持目前的Cobre和UofL健康科学中心(HSC)项目。 2)对学员和其他调查人员进行教育和培训。 3)开发新的协议和技术。 4)成为Cobre和UofL HSC的自给自足的特征。