Biorepository and Animal Core

项目摘要摘要-动物模型和生物库核心（核心D） 肝脏生物学和毒理学中心的动物模型和生物储存库核心（核心D）提供了 重要的肝脏研究所需的基本专业知识，设施，资源和标准化服务。的 核心重点是COBRE初级调查员的需求，但它也为其他中心成员提供服务， 肝脏研究者该核心增强了基础设施以及技术和学术能力， 中心和整个机构。Core D的主要功能是为COBRE成员提供访问 （i）肝脏疾病的动物模型（包括这些模型的表征）;（ii）原代肝细胞;和 (iii)先前从患有肝病的人类受试者中采集的去识别生物标本。 核心的实验模型服务包括：动物暴露;饲养;尸检与组织收集， 处理和生物储存;表型和肝组织学;以及分离原发性肝细胞 用于离体和体外研究的细胞。核心将协助COBRE成员开发新的实验性 动物模型和体外系统来研究肝病。核心小组将作为与非重复小组的联络机构， UofL的另外两个COBRE中心的动物研究资源，以最大限度地提高整体影响。核心人员将 确保饲养以及样品收集、处理和评价的统一性， 在每个模型中测量特性。这种一致性确保了COBRE中生成的数据 是一致的，一个实验的结果将与另一个实验的结果直接可比。的 核心的人类生物库可以访问以前收集的去识别生物的独特库存 来自患有肝病的受试者的样品（例如，酒精性肝病，职业性/环境性肝病 疾病、肝癌等）以促进转化研究。核心D将协助COBRE成员， 为他们的研究获得必要的监管批准。除了作为服务核心的功能外，Core D还将 促进COBRE初级调查员和成员的教育和培训。 作为COBRE的重要组成部分，不仅为其成员提供服务， 就整个机构而言，核心D有以下具体目标：1）促进发展和成功 当前和未来的COBRE项目。2)教育和培训调查人员。3)开发新的协议， 技术. 4)成为COBRE不可或缺的自我维持的组成部分。

Project Summary Abstract – Animal Model and Biorepository Core (Core D) The Animal Model and Biorepository Core (Core D) of the Hepatobiology and Toxicology Center provides the essential expertise, facilities, resources, and standardized services required for impactful liver research. The Core focuses on the needs of the COBRE’s junior investigators, but it also serves other Center members and liver investigators. The Core enhances infrastructure as well as the technical and academic capabilities of the Center and the Institution as a whole. Core D’s primary functions are to provide COBRE members with access to: (i) animal models of liver disease (including the characterization of these models); (ii) primary liver cells; and (iii) previously collected, de-identified biological specimens from human subjects with liver disease. The Core’s experimental model services include: animal exposure; husbandry; necropsy with tissue collection, processing and biorepository storage; phenotyping and liver histology; as well as the isolation of primary hepatic cells for ex vivo and in vitro studies. The Core will assist COBRE members in developing new experimental animal models and in vitro systems to study liver disease. The Core will serve as a liaison to non-duplicative animal research resources at UofL’s two other COBRE Centers to maximize overall impact. Core personnel will ensure uniformity of husbandry as well as sample collection, processing and evaluation so that the same set of characteristics is measured in each model. This uniformity ensures that the data generated within the COBRE are consistent and that results of one experiment will be directly comparable with the results of another. The Core’s human biorepository enables access to a unique inventory of previously collected de-identified biological samples from subjects with liver diseases (e.g., alcoholic liver disease, occupational/environmental liver diseases, liver cancer, etc.) in order to promote translational research. Core D will assist COBRE members with the necessary regulatory approvals for their research. In addition to its function as a service core, Core D will promote the education and training of the COBRE junior investigators and members. To function as a vital integrative component of the COBRE providing services not only for its members but also for the Institution as a whole, Core D has the following Specific Aims: 1) To foster the development and success of current and future COBRE projects. 2) To educate and train investigators. 3) To develop new protocols and techniques. 4) To become an integral and self-sustaining component of the COBRE.