Biorepository and Animal Core

项目摘要-动物模型和生物库核心(核心D) 肝脏生物学和毒理学中心的动物模型和生物储存库核心(核心D)提供 有效的肝脏研究所需的基本专业知识、设施、资源和标准化服务。这个 CORE专注于科布雷初级调查人员的需求，但它也服务于中心的其他成员和 肝脏调查员。核心增强了基础设施以及技术和学术能力 中心和学院作为一个整体。核心D的主要功能是为Cobre成员提供访问 (I)肝病动物模型(包括这些模型的特征)；(Ii)原代肝细胞；以及 (Iii)以前从患有肝病的人类受试者身上收集的、未经鉴定的生物标本。 中心的实验模型服务包括：动物暴露；畜牧业；组织收集的尸检， 加工和生物储存库；表型和肝脏组织学；以及初级肝脏的分离 用于体外和体外研究的细胞。核心将协助Cobre成员开发新的实验 用于研究肝病的动物模型和体外系统。核心将作为非复制的联络人 UofL的另外两个Cobre中心的动物研究资源，以最大限度地提高整体影响。核心人员将 确保畜牧业和样品采集、处理和评价的一致性，使同一套 每种型号的特性都进行了测量。这种一致性确保了在Cobre中生成的数据 是一致的，一个实验的结果将直接与另一个实验的结果相比较。这个 CORE的人类生物库使人们能够访问之前收集的未识别的生物的独特清单 来自肝病患者的样本(例如酒精性肝病、职业性/环境性肝脏 疾病、肝癌等)以促进翻译研究。CORE D将协助Cobre成员 为他们的研究获得必要的监管批准。除了作为服务核心的功能外，核心D还将 促进科布雷初级调查人员和成员的教育和培训。 作为COBRE的重要组成部分，不仅为其成员提供服务，而且还 就整个机构而言，核心D有以下具体目标：1)促进发展和成功 当前和未来的科布雷项目。2)对侦查人员进行教育和培训。3)开发新的协议和 技巧。4)成为科布雷不可或缺的自给自足的组成部分。

Project Summary Abstract – Animal Model and Biorepository Core (Core D) The Animal Model and Biorepository Core (Core D) of the Hepatobiology and Toxicology Center provides the essential expertise, facilities, resources, and standardized services required for impactful liver research. The Core focuses on the needs of the COBRE’s junior investigators, but it also serves other Center members and liver investigators. The Core enhances infrastructure as well as the technical and academic capabilities of the Center and the Institution as a whole. Core D’s primary functions are to provide COBRE members with access to: (i) animal models of liver disease (including the characterization of these models); (ii) primary liver cells; and (iii) previously collected, de-identified biological specimens from human subjects with liver disease. The Core’s experimental model services include: animal exposure; husbandry; necropsy with tissue collection, processing and biorepository storage; phenotyping and liver histology; as well as the isolation of primary hepatic cells for ex vivo and in vitro studies. The Core will assist COBRE members in developing new experimental animal models and in vitro systems to study liver disease. The Core will serve as a liaison to non-duplicative animal research resources at UofL’s two other COBRE Centers to maximize overall impact. Core personnel will ensure uniformity of husbandry as well as sample collection, processing and evaluation so that the same set of characteristics is measured in each model. This uniformity ensures that the data generated within the COBRE are consistent and that results of one experiment will be directly comparable with the results of another. The Core’s human biorepository enables access to a unique inventory of previously collected de-identified biological samples from subjects with liver diseases (e.g., alcoholic liver disease, occupational/environmental liver diseases, liver cancer, etc.) in order to promote translational research. Core D will assist COBRE members with the necessary regulatory approvals for their research. In addition to its function as a service core, Core D will promote the education and training of the COBRE junior investigators and members. To function as a vital integrative component of the COBRE providing services not only for its members but also for the Institution as a whole, Core D has the following Specific Aims: 1) To foster the development and success of current and future COBRE projects. 2) To educate and train investigators. 3) To develop new protocols and techniques. 4) To become an integral and self-sustaining component of the COBRE.