3D imaging of cells and tissues using polarized light microscopy
3D imaging of cells and tissues using polarized light microscopy
批准号:
10381555
负责人:
RUDOLF OLDENBOURG
金额:
$50.18万
依托单位国家:
美国
项目类别:
财政年份:
2019
资助国家:
美国
项目状态:
已结题
起止时间:
2019-06-01 至 2024-03-31
关键词:
3-DimensionalAlgorithmic SoftwareArchitectureBiologicalBirefringenceCell divisionCellsClinicalCollagenComputer softwareDataDevelopmentDevice or Instrument DevelopmentDiseaseEukaryotic CellFibroblastsFluorescence PolarizationGrantHealthImageImaging TechniquesLightLight MicroscopeLightingMapsMeasurementMedical ResearchMicroscopeMolecularPolarization MicroscopyProcessResolutionStructureTechniquesTechnologyThree-Dimensional ImagingTimeTissuesWhole Organismcellular imagingdesignextracellularinstrumentinstrumentationpolarized lightreconstructiontomographyweb portal
中文摘要
项目摘要
我们建议继续发展偏振光显微镜技术,
活细胞、组织和整个有机体内部的结构动力学。我们建议增加全面
偏振分析到两个多视图成像技术,双视图平面照明显微镜,或
diSPIM和光场显微镜。这两种类型的偏振光显微镜代表了重要的新
发展,第一次使测量分子取向参数在三个
高空间分辨率(所有三个维度均为0.5µm)和时间分辨率(快于1Hz)。的
该项目包括硬件,软件和算法的开发所需的捕捉多视图
成像数据及其层析重建成双折射和偏振三维图
荧光。仪器的开发是由几个应用项目,包括改造
成纤维细胞的细胞外胶原基质及其高级组装中的空间协调
真核细胞内的septin结构。有关技术的信息,包括软件组件和
仪器设计是根据这项赠款开发,将提供给用户和开发人员在一个
建立了名为OpenPolScope. org的门户网站。
英文摘要
Project Summary
We are proposing to continue our development of polarized light microscopy techniques for analyzing the
architectural dynamics inside living cells, tissues and whole organisms. We propose to add comprehensive
polarization analysis to two multi-view imaging techniques, the dual-view plane illumination microscope, or
diSPIM, and the light-field microscope. Both types of polarized light microscopes represent significant new
developments that for the first time enable the measurement of molecular orientation parameters in three
dimensions at high spatial (0.5µm in all three dimensions) and temporal (faster than 1Hz) resolution. The
project includes the development of the hardware, software and algorithms required for capturing multi-view
image data and their tomographic reconstruction into 3-dimensional maps of birefringence and polarized
fluorescence. The instrument development is guided by several application projects including the remodeling of
the extracellular collagen matrix by fibroblast cells and the spatial coordination in the assembly of higher order
septin structures inside eukaryotic cells. Information about the technology, including software components and
instrument design that are developed under this grant, will be made available for users and developers on an
established web portal called OpenPolScope.org.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
3D imaging of cells and tissues using polarized light microscopy
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批准号:10597635
-
项目类别:
-
资助金额:$50.18万
-
财政年份:2019
-
负责人:RUDOLF OLDENBOURG
-
依托单位:
Instantaneous 3D imaging of cells and tissues using polarized light microscopy
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批准号:9527370
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项目类别:
-
资助金额:$10.91万
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财政年份:2015
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负责人:RUDOLF OLDENBOURG
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依托单位:
Instantaneous 3D imaging of cells and tissues using polarized light microscopy
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批准号:8863961
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项目类别:
-
资助金额:$58.42万
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财政年份:2015
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负责人:RUDOLF OLDENBOURG
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依托单位:
CELL DIVISION AND RELATED MOTILITY
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批准号:7721087
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项目类别:
-
资助金额:$0.56万
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财政年份:2007
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负责人:RUDOLF OLDENBOURG
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依托单位:
CELL DIVISION AND RELATED MOTILITY
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批准号:7598493
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项目类别:
-
资助金额:$0.59万
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财政年份:2006
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负责人:RUDOLF OLDENBOURG
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依托单位:
CELL DIVISION AND RELATED MOTILITY
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批准号:7357339
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项目类别:
-
资助金额:$0.12万
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财政年份:2005
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负责人:RUDOLF OLDENBOURG
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依托单位:
CELL DIVISION AND RELATED MOTILITY
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批准号:6980019
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项目类别:
-
资助金额:$0.19万
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财政年份:2003
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负责人:RUDOLF OLDENBOURG
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依托单位:
3-D Image Restoration for Polarized Light Microscopy
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批准号:6400765
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项目类别:
-
资助金额:$15.33万
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财政年份:2001
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负责人:RUDOLF OLDENBOURG
-
依托单位:
3-D Image Restoration for Polarized Light Microscopy
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批准号:6656953
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项目类别:
-
资助金额:$25.29万
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财政年份:2001
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负责人:RUDOLF OLDENBOURG
-
依托单位:
3-D Image Restoration for Polarized Light Microscopy
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批准号:6526004
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项目类别:
-
资助金额:$23.0万
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财政年份:2001
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负责人:RUDOLF OLDENBOURG
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依托单位:
ACTIN BUNDLES IN NEURONAL GROWTH CONE OBSERVED W/ OLDENBOURGS NEW POL SCOPE
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批准号:6319686
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项目类别:
-
资助金额:$0.69万
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财政年份:1998
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负责人:RUDOLF OLDENBOURG
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依托单位:--
DEVELOPMENT OF NEW AUTOMATED POLARIZED LIGHT MICROSCOPE
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批准号:2186754
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项目类别:
-
资助金额:$21.03万
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财政年份:1992
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负责人:RUDOLF OLDENBOURG
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依托单位:
DEVELOPMENT OF NEW AUTOMATED POLARIZED LIGHT MICROSCOPE
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批准号:2459483
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项目类别:
-
资助金额:$32.0万
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财政年份:1992
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负责人:RUDOLF OLDENBOURG
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依托单位:
DEVELOPMENT OF NEW AUTOMATED POLARIZED LIGHT MICROSCOPE
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批准号:3308560
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项目类别:
-
资助金额:$21.8万
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财政年份:1992
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负责人:RUDOLF OLDENBOURG
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依托单位:
DEVELOPMENT OF NEW AUTOMATED POLARIZED LIGHT MICROSCOPE
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批准号:2849734
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项目类别:
-
资助金额:$8.33万
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财政年份:1992
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负责人:RUDOLF OLDENBOURG
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依托单位:
Advanced Optical Methods in Cell Biology
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批准号:8101078
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项目类别:
-
资助金额:$49.52万
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财政年份:1992
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负责人:RUDOLF OLDENBOURG
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依托单位:
DEVELOPMENT OF AUTOMATED POLARIZED LIGHT MICROSCOPE
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批准号:6052377
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项目类别:
-
资助金额:$41.28万
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财政年份:1992
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负责人:RUDOLF OLDENBOURG
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依托单位:
Development of New Automated Polarized Light Microscope
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批准号:6915169
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项目类别:
-
资助金额:$49.35万
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财政年份:1992
-
负责人:RUDOLF OLDENBOURG
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依托单位:
Advanced Optical Methods in Cell Biology
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批准号:8303238
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项目类别:
-
资助金额:$44.32万
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财政年份:1992
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负责人:RUDOLF OLDENBOURG
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依托单位:
Advanced Optical Methods in Cell Biology
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批准号:7735493
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项目类别:
-
资助金额:$46.9万
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财政年份:1992
-
负责人:RUDOLF OLDENBOURG
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依托单位:
海外基金