CELL DIVISION AND RELATED MOTILITY

细胞分裂和相关运动

• 批准号: 7357339
• 负责人: RUDOLF OLDENBOURG
• 金额: $ 0.12万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357339
• 关键词: CELL; DIVISION; RELATED; MOTILITY

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Members of the Architectural Dynamics in Living Cells (ADLC) Program at the MBL are benefiting of facilities and interactions with staff members of the Biocurrent Research Center (BRC). Specifically, ADLC members use the cell culture facility for maintaining cultures of immortalized Drosophila cells for studying cell division and related motility. Cell cultures of Drosophila Kc cells are subjected to small interfering RNA to suppress the expression of specific proteins such as centrosomin. Cells that lack centrosomin lack mitotic centrosomes which are usually located at the spindle poles. Live Kc cells dividing under those conditions are non- invasively observed using advanced microscopy instrumentation that is being developed in the ADLC Program.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。MBL活细胞建筑动力学（ADLC）项目的成员受益于生物电流研究中心（BRC）的设施和工作人员的互动。具体来说，ADLC成员使用细胞培养设施来维持永生化果蝇细胞的培养，以研究细胞分裂和相关运动。对果蝇Kc细胞的细胞培养物进行小干扰RNA以抑制特定蛋白质如中心体蛋白的表达。缺乏中心体蛋白的细胞缺乏有丝分裂中心体，中心体通常位于纺锤体两极。在这些条件下分裂的活Kc细胞使用ADLC计划中正在开发的先进显微镜仪器进行非侵入性观察。