Instantaneous 3D imaging of cells and tissues using polarized light microscopy

使用偏光显微镜对细胞和组织进行瞬时 3D 成像

• 批准号: 8863961
• 负责人: RUDOLF OLDENBOURG
• 金额: $ 58.42万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8863961
• 关键词: 3-Dimensional; Accounting; Algorithmic Software; Algorithms; Anisotropy; Architecture; Area; Biological; Biological Models; Biology; Birefringence; Cell division; Cells; Chromosomes; Clinical; Clinical Research; Collaborations; Collagen; Computer software; Data; Development; Device or Instrument Development; Dimensions; Disease; Eukaryotic Cell; Fibroblasts; Fluorescence; Grant; Health; Image; Internet; Investigation; Laboratories; Length; Life; Light; Maps; Measures; Medical Research; Medicine; Meiosis; Methods; Microscope; Microscopic; Microscopy; Molecular; Nature; Optics; Pattern; Photophobia; Polarization Microscopy; Polymers; Process; Property; Qualifying; Refractive Indices; Resolution; Shapes; Solid; Specimen; Spermatocytes; Structure; Techniques; Technology; Theoretical Studies; Three-Dimensional Imaging; Three-dimensional analysis; Time; Tissues; Uncertainty; absorption; attenuation; beam-splitter; design; extracellular; fluorescence imaging; fly; imaging modality; insight; instrument; instrumentation; liquid crystal; new technology; novel strategies; polarized light; public health relevance; reconstruction; repaired; research study; sensor; submicron; tomography; vector

 DESCRIPTION (provided by applicant): We are proposing to develop the polarized light field microscope for analyzing the architectural dynamics inside living cells, tissues and cell-free model systems. The instrument combines comprehensive polarization analysis and 3D imaging of microscopic specimens in a single snapshot using the new approach of light field imaging. The project includes the development of the hardware, software and algorithms required for capturing polarized light field images and their tomographic reconstruction into 3-dimensional maps of birefringence, diattenuation and polarized fluorescence. The instrument development is guided by several application projects including the remodeling of the extracellular collagen matrix by fibroblast cells, the spatial coordination in the assembly of higher order septin structures inside eukaryotic cells and the origins of chromosome malorientations and their repair in the meiotic spindle of spermatocytes. Information about the technology, including software components and instrument design that are developed under this grant, will be made available for users and developers on an established web portal called OpenPolScope.org.

 描述(申请人提供)：我们建议开发偏振光场显微镜，用于分析活细胞、组织和无细胞模型系统内的结构动力学。该仪器使用新的光场成像方法，在一个快照中结合了全面的偏振分析和显微样品的3D成像。该项目包括开发捕捉偏振光场图像并将其层析重建成双折射、双衰减和偏振荧光的三维地图所需的硬件、软件和算法。该仪器的开发是由几个应用项目指导的，包括成纤维细胞对细胞外胶原基质的重塑，真核细胞内高阶间隔蛋白结构组装的空间协调，以及精母细胞减数分裂纺锤体中染色体取向错误的起源及其修复。关于这项技术的信息，包括根据这笔赠款开发的软件组件和仪器设计，将在一个名为OpenPolScope e.org的已建立的门户网站上向用户和开发人员提供。