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3-D Image Restoration for Polarized Light Microscopy

偏光显微镜的 3D 图像恢复

基本信息

批准号：
6656953
负责人：
RUDOLF OLDENBOURG
金额：
$ 25.29万
依托单位：
MARINE BIOLOGICAL LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-09-15 至 2004-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6656953
关键词：
automated data processing bioengineering /biomedical engineering bioimaging /biomedical imaging birefringences cell component structure /function computer program /software image processing light microscopy mathematics optical polarization structural biology

项目摘要

DESCRIPTION (provided by applicant): We have been developing and applying a new type of polarized light microscope (the P01-Scope) which dramatically enhances the analytic power of the traditional polarizing microscope. The P01-Scope incorporates a universal compensator, made from computer driven liquid crystal devices, to measure the birefringent fine structure at high sensitivity (0.02 nm birefringence retardation), high resolution (0.2 pm), simultaneously over the whole field of view, and for all orientations of the birefringence axis within the focus plane. A 3-dimensional birefringent specimen, such as a living cell, is imaged with the Pol-Scope as a series of optical Sections. Each section contains the birefringence of in-focus structures, such as membranous stacks, aligned filament arrays and individual filament bundles. However, the measurements of in-focus birefringences have spurious contributions from out-of focus cellular components. The structural analysis of the detailed and quantitative information afforded by the P01-Scope is often limited because of these spurious birefringence contributions. Therefore, to take full advantage of the quantitative Pol-Scope images, the out-of-focus information needs to be identified and removed. We are proposing to develop a computational restoration procedure to remove the effect of out-of-focus components on the measurements of in-focus birefringences. The P01-Scope restoration algorithm will be based on procedures established for fluorescence microscopy that we will modify to account for the specific properties of partial coherent imaging with polarized light. The first development goal is to establish a forward procedure that is used to compute image features based on measured point spread functions and known or estimated positions, magnitudes and axis orientations of birefringent objects. The second goal is to find the inverse procedure that optimizes a numerical estimate of object birefringences in volume elements that are contained in P01-Scope optical sections. The inverse procedure iteratively minimizes the difference between experimental and computed images. This process ends in a stable 3-D distribution of positions, magnitudes and axis orientations of specimen birefringences. The image restoration procedures will greatly enhance the utility of the P01-Scope for biological and medical applications to non-invasively analyze the architectural dynamics of living cells.

描述（由申请人提供）：我们一直在开发和应用一种新的

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Polarized light microscopy in reproductive and developmental biology.

DOI：
10.1002/mrd.22221
发表时间：
2015-07
期刊：
Molecular reproduction and development
影响因子：
2.5
作者：
Koike-Tani M;Tani T;Mehta SB;Verma A;Oldenbourg R
通讯作者：
Oldenbourg R

pH dependent isotropic to nematic phase transitions in graphene oxide dispersions reveal droplet liquid crystalline phases.

DOI：
10.1039/c4cc00970c
发表时间：
2014-05
期刊：
Chemical communications
影响因子：
4.9
作者：
Rachel Tkacz;R. Oldenbourg;Shalin B. Mehta;Morteza Miansari;Amitabh Verma;M. Majumder
通讯作者：
Rachel Tkacz;R. Oldenbourg;Shalin B. Mehta;Morteza Miansari;Amitabh Verma;M. Majumder

Polarized light microscopy: principles and practice.

偏光显微镜：原理与实践。