CELL DIVISION AND RELATED MOTILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 MBL的活细胞建筑动力学(ADLC)项目的成员正在受益于生物水流研究中心(BRC)的设施和与工作人员的互动。具体地说，ADLC成员使用细胞培养设施来维持永生化果蝇细胞系的培养，以研究细胞分裂和相关的运动性。培养的果蝇KC细胞受到小干扰RNA抑制特定蛋白的表达，如中心体蛋白。缺乏中心体的细胞缺乏有丝分裂的中心体，中心体位于纺锤体的两极。在这些条件下分裂的活的KC细胞是使用ADLC计划正在开发的偏振光显微镜技术进行非侵入性观察的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Members of the Architectural Dynamics in Living Cells (ADLC) Program at the MBL are benefiting of facilities and interactions with staff members of the Biocurrent Research Center (BRC). Specifically, ADLC members use the cell culture facility for maintaining cultures of immortalized Drosophila cell lines for studying cell division and related motility. Cell cultures of Drosophila Kc cells are subjected to small interfering RNA to suppress the expression of specific proteins such as centrosomin. Cells that lack centrosomin lack mitotic centrosomes, which are located at the spindle poles. Live Kc cells dividing under those conditions are non-invasively observed using polarized light microscopy techniques that are being developed in the ADLC Program.