CELL DIVISION AND RELATED MOTILITY

细胞分裂和相关运动

• 批准号: 7721087
• 负责人: RUDOLF OLDENBOURG
• 金额: $ 0.56万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721087
• 关键词: Cell Line; Cell division; Cells; Centrosome; Computer Retrieval of Information on Scientific Projects Database; Condition; Cultured Cells; Drosophila genus; Funding; Grant; Institution; Life; Mannose Binding Lectin; Mitotic; Polarization Microscopy; Proteins; Research; Research Personnel; Resources; Small Interfering RNA; Source; Techniques; United States National Institutes of Health; cell motility; member; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Members of the Architectural Dynamics in Living Cells (ADLC) Program at the MBL are benefiting of facilities and interactions with staff members of the Biocurrent Research Center (BRC). Specifically, ADLC members use the cell culture facility for maintaining cultures of immortalized Drosophila cell lines for studying cell division and related motility. Cell cultures of Drosophila Kc cells are subjected to small interfering RNA to suppress the expression of specific proteins such as centrosomin. Cells that lack centrosomin lack mitotic centrosomes, which are located at the spindle poles. Live Kc cells dividing under those conditions are non-invasively observed using polarized light microscopy techniques that are being developed in the ADLC Program.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 MBL活细胞建筑动力学（ADLC）项目的成员受益于生物电流研究中心（BRC）的设施和工作人员的互动。具体来说，ADLC成员使用细胞培养设施来维持永生化果蝇细胞系的培养，以研究细胞分裂和相关运动。对果蝇Kc细胞的细胞培养物进行小干扰RNA以抑制特定蛋白质如中心体蛋白的表达。缺乏中心体蛋白的细胞缺乏有丝分裂中心体，中心体位于纺锤体两极。使用ADLC计划中正在开发的偏振光显微镜技术，非侵入性地观察在这些条件下分裂的活Kc细胞。