Tractable Tandem Ion Mobility Technology using Structures for Lossless Ion Manipulations and Photodissociation

使用无损离子操作和光解离结构的易处理串联离子淌度技术

• 批准号: 10386669
• 负责人: Brian Clowers
• 金额: $ 19.99万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10386669
• 关键词: Address; Administrative Supplement; Adoption; Biological; Complement; Data; Diagnostic; Dimensions; Discipline; Disease; Dissociation; Electrodes; Electronics; Gases; Goals; Ions; Mass Spectrum Analysis; Measurement; Modality; Molecular; Molecular Conformation; Nature; Performance; Phase; Photons; Population; Research; Research Personnel; Resolution; Role; Series; Spectrometry; Speed; Structure; System; Techniques; Technology; Time; Transcend; Travel; Variant; analog; biological systems; biophysical properties; cost effective; data acquisition; density; electric field; experimental study; innovation; insight; ion mobility; mass analyzer; metabolomics; molecular dynamics; molecular shape; pressure; printed circuit board; programs; stereochemistry; structural biology; time of flight mass spectrometry; ultra high resolution; ultraviolet

Tractable Tandem Ion Mobility Technology using Structures for Lossless Ion Manipulations and Photodissociation Administrative Supplement NOT-GM-21-030 R01GM140129 Summary. Broadly available advances in mass spectrometry (MS), with unparalleled levels of selectivity, speed, and sensitivity, have armed researchers with new biological insights and prompt additional questions regarding molecular and biophysical parameters that differentiate disease states but transcend MS measurements. Ion mobility spectrometry (IMS) is a gas-phase separation technique that directly complements MS measurements and expands understanding regarding molecular shape and dynamics in biological systems. Using widely available technological advances in the field of printed circuit board (PCB) manufacturing, a new class of ion mobility separation is enabled that largely alleviates the drawbacks of its predecessors. The Structures for Lossless Ion Manipulations (SLIM) framework achieves this goal by establishing a dynamic electric field capable of confining ionized molecules for extended periods of time along with a means to efficiently fractionate the different classes prior to analysis using MS. To cast the SLIM platform into multiple separation dimensions and achieve new levels of biologically relevant diagnostics, the present effort aims to develop and disseminate an economical tandem IMS platform that integrates a series of innovative, simplifying strategies. Most importantly, and prior to MS analysis, we will exploit the highly compressed nature of the ion beams within the SLIM by subjecting these species to high intensity ultraviolet (UV) photons to induce molecular disruption and yield more information regarding the target biological system. With the added functionality of tandem IMS experiment, the separation power of the system is anticipated to represent the state-of-the-art. At the conclusion of the proposed research we expect to realize a fully functioning, high-efficiency SLIM-UV photodissociation framework capable of interfacing to all mass analyzer classes and ready to address a suite of biological problems ranging from metabolomics to structural biology. Administrative Supplement Request. To fully accommodate the high-density ion populations produced by the SLIM platform and maximize experimental accuracy, an ioniTOF 4000 time-of-flight (TOF) MS equipped with an analog data conversion (ADC) system is requested. In addition to enhancing the pace of each SLIM experiment, this instrumental advance promises to promote the rapid incorporation of the SLIM-UV technology into larger bioanalytical campaigns. The requested TOF-MS and its associated data acquisition system directly address the core limitations of the present mass analyzer used in the project and will facilitate experimental efforts supporting Aims 1, 2, and 3.

易处理串联离子迁移技术， 无损离子操纵和光解离的结构 行政补充文件NOT-GM-21-030 R 01 GM 140129总结。质谱法（MS）的广泛应用进展， 选择性，速度和灵敏度，武装了研究人员与新的生物学见解和迅速增加 关于区分疾病状态但超越MS的分子和生物物理参数的问题 测量.离子迁移谱（IMS）是一种气相分离技术， MS测量并扩展了对生物系统中分子形状和动力学的理解。 利用印刷电路板（PCB）制造领域广泛应用的技术进步，一种新的 这类离子迁移率分离能够在很大程度上克服其前代的缺点。的 无损离子操纵结构（SLIM）框架通过建立动态电 场，其能够将电离分子限制延长的时间段沿着， 在使用MS进行分析之前，对不同的类进行分段。 尺寸和实现生物相关诊断的新水平，目前的努力旨在开发和 传播一个经济的串联IMS平台，该平台集成了一系列创新的简化战略。 最重要的是，在MS分析之前，我们将利用内部离子束的高度压缩特性， SLIM通过使这些物质经受高强度紫外（UV）光子以诱导分子破裂 并产生关于目标生物系统的更多信息。随着串联IMS功能的增加， 实验表明，该系统的分离能力有望代表最新技术水平 我们希望实现一个功能齐全，高效的SLIM-UV光解离 一个能够与所有质量分析器类接口的框架，并准备解决一系列生物学问题 从代谢组学到结构生物学。 行政补充申请。为了完全容纳由离子产生的高密度离子群体， SLIM平台和最大限度地提高实验精度，配备了一个电离飞行时间（TOF）4000 MS， 模拟数据转换（ADC）系统。除了加快每个SLIM实验的步伐外， 这一技术上的进步，有望促进超视距激光干涉成像-紫外技术在更大的航天器上的快速应用， 生物分析活动。所请求的TOF-MS及其相关数据采集系统直接寻址 该项目中使用的现有质量分析仪的核心局限性，并将促进实验工作， 目标1、2和3。