IN-VIVO SKIN IMAGING

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 本项目的主要目标是开发一种稳健的人体皮肤成像的非线性显微镜方法。这将包括激发/发射参数的优化、成像深度的改善(即皮肤的光学清晰度、物镜探测器的灵敏度)和运动伪影的预防。 成像目标有两个方面。首先，皮肤表面更多的细胞层(角质层和表皮，形成了50-75米的皮肤)首当其冲地承受着皮肤癌的发生：黑素细胞位于基底表皮层，表皮负责基底和鳞状细胞癌以及良性病变(光化性角化病、脂溢性角化病等)的发生。这是保护身体免受微生物、水分、紫外线等环境因素影响的第一道屏障。 底层真皮(厚度2-4毫米，取决于身体部分)由结缔组织组成，结缔组织是由弹性和胶原纤维组成的细胞外基质，其细胞成分负责伤口愈合和免疫反应等。 人的皮肤反映了年龄、性别、种族、健康状况。具有显微分辨率和光谱选择性的皮肤成像可以为了解伤口愈合、癌症发生、衰老和环境响应等重要的生物和生物力学过程提供强有力的工具。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The main goal of this project is to develop a robust method of non-linear microscopy of human skin imaging. This will include the excitation/emission parameters optimization, depth of imaging improvement (i.e. optical clearing of skin, objective-detector sensitivity) and motion artifacts prevention. The imaging goals are two-fold. First, the more cellular topical skin layers (stratum corneum and epidermis that form fist 50-75 ¿m of skin) are bearing the main brunt of skin carcinogenesis: melanocytes are localized within the basal epidermal layer, epidermis is responsible for basal and squamous cell carcinoma as well as benign lesions (actinic keratosis, sebhorreic keratosis, etc) genesis. This is a first barrier protecting the body from environmental factors such as microorganisms, moisture, UV, etc. The underlaying dermis (2-4 mm thickness depending on the body part) bulk is made of connective tissue, a ECM of elastic and collagen fibers, cellular component of which is responsible for wound healing and immune response among others. Human skin reflects age, gender, race, health status. Imaging skin with microscopic resolution and spectral selectivity can provide a powerful tool in understanding important biological and biomechanical processes of wound healing, carcinogenesis, aging, and environmental response.