MULTI PHOTON MICROSCOPY SIGNAL ORIGIN (SHG VS FLUORESCENCE)

多光子显微镜信号起源（SHG VS 荧光）

• 批准号: 8362592
• 负责人: Bruce J. Tromberg
• 金额: $ 3.11万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362592
• 关键词: Biotechnology; Collagen; Fibroblasts; Fluorescence; Funding; Generations; Grant; Image; Immersion Investigative Technique; Lasers; Microscope; Microscopy; NADH; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Signal Transduction; Source; System; Tissue Model; Tissues; United States National Institutes of Health; Water; Work; cost; macrophage; multi-photon; protoporphyrin IX; submicron; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In the past year, using the multi-photon microscope (MPM) system constructed in the LAMMP facility, we were able to image both collagen and NADH autofluorescence collagen second hormonic generation (SHG) and Protoporphyrin IX localization (exogenous fluorescence) within a RAFT tissue model. Images of the RAFT tissue, composed of collagen, macrophages and fibroblasts, were obtained with submicron resolution to a depth of 200 mm, the working distance of the water immersion objective used. Selective uptake of Protoporphyrin IX by specific cellular components of the matrix was also visualized at depth utilizing the TPM system.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 在过去的一年中，使用在LAMMP设施中构建的多光子显微镜（MPM）系统，我们能够在RAFT组织模型中对胶原蛋白和NADH自发荧光胶原蛋白第二代（SHG）和原卟啉IX定位（外源性荧光）进行成像。 RAFT组织的图像，包括胶原蛋白，巨噬细胞和成纤维细胞，获得了亚微米分辨率的深度为200毫米，所使用的水浸物镜的工作距离。 选择性摄取原卟啉IX的特定细胞成分的矩阵也可视化在深度利用TPM系统。