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Mechanism of Microbial DNA Hypervariation through Mutagenic Transposition

通过诱变转座导致微生物 DNA 高度变异的机制

基本信息

批准号：
10387714
负责人：
Donald H Burke
金额：
$ 9.21万
依托单位：
UNIVERSITY OF MISSOURI-COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-19 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10387714
关键词：
Address Adenine Amino Acid Substitution Archaea Bacteria Bacteriophages Base Pairing Biochemical Bioinformatics Biomedical Engineering Bordetella Chemicals Complementary DNA Complex DNA Detection Development Dyes Elements Environment Family Funding Genes Genetic Genetic Polymorphism Homing Human Hybrids Integration Host Factors Investigation Label Light Liquid substance Mediating Metagenomics Methodology Molecular Mutagenesis Mutation Nucleotides Oligonucleotides Paper Performance Phylogenetic Analysis Play Preparation Process Property Proteins RNA RNA Sequences RNA primers RNA-Directed DNA Polymerase Reaction Request for Proposals Research Retroelements Reverse Transcription Ribonucleoproteins Role Site Structure Tropism Variant Virus Work in vivo instrument instrumentation laboratory equipment macromolecule medical specialties microbial microorganism nucleic acid binding protein nucleotide analog parent grant receptor small molecule

项目摘要

This proposal requests funds for a high-performance liquid chromatograph (HPLC) that is suitable for both analytical and preparative work, as a major instrumentation supplement in support of the parent grant R01GM125669, Mechanism of Microbial DNA Hypervariation through Mutagenic Transposition. A highcaliber HPLC is a critical piece of standard laboratory equipment for almost any cutting edge biochemical research. This instrument will be used in preparative capacity for purification of chemically-modified oligonucleotides (such as dye-labeled primers and site-specifically modified DGR cDNA intermediates), proteins (such as bRT and bAvd from Bordatella phage and from phylogenetically related phage), and specialty small molecules (such as modified nucleotide analogs). We would also use the instrument in analytical capacity for quantitative assessment of chemical purity of reaction products involving small molecules, macromolecules, and conjugated variants of the macromolecules.

该提案要求提供资金，以购买一台适用于以下两种情况的高效液相色谱仪（HPLC）：分析和准备工作，作为支持母公司赠款的主要工具补充 R01GM125669，通过诱变转座的微生物DNA超变机制。高精度HPLC是几乎所有尖端生化实验室标准设备的关键部件 research.该仪器将用于制备能力，用于纯化化学改性的寡核苷酸（如染料标记的引物和位点特异性修饰的DGR cDNA 中间体）、蛋白质（如来自博德特氏菌噬菌体和来自细菌遗传学相关的噬菌体的bRT和bAvd）、噬菌体）和特异性小分子（如修饰的核苷酸类似物）。我们还将使用定量评价反应产物化学纯度的分析仪器涉及小分子、大分子和大分子的共轭变体。