Mechanism of Microbial DNA Hypervariation through Mutagenic Transposition

该提案要求提供资金，以购买一台适用于以下两种情况的高效液相色谱仪（HPLC）： 分析和准备工作，作为支持母公司赠款的主要工具补充 R01GM125669，通过诱变转座的微生物DNA超变机制。高精度HPLC是几乎所有尖端生化实验室标准设备的关键部件 research.该仪器将用于制备能力，用于纯化化学改性的 寡核苷酸（如染料标记的引物和位点特异性修饰的DGR cDNA 中间体）、蛋白质（如来自博德特氏菌噬菌体和来自细菌遗传学相关的噬菌体的bRT和bAvd）、 噬菌体）和特异性小分子（如修饰的核苷酸类似物）。我们还将使用 定量评价反应产物化学纯度的分析仪器 涉及小分子、大分子和大分子的共轭变体。

This proposal requests funds for a high-performance liquid chromatograph (HPLC) that is suitable for both analytical and preparative work, as a major instrumentation supplement in support of the parent grant R01GM125669, Mechanism of Microbial DNA Hypervariation through Mutagenic Transposition. A highcaliber HPLC is a critical piece of standard laboratory equipment for almost any cutting edge biochemical research. This instrument will be used in preparative capacity for purification of chemically-modified oligonucleotides (such as dye-labeled primers and site-specifically modified DGR cDNA intermediates), proteins (such as bRT and bAvd from Bordatella phage and from phylogenetically related phage), and specialty small molecules (such as modified nucleotide analogs). We would also use the instrument in analytical capacity for quantitative assessment of chemical purity of reaction products involving small molecules, macromolecules, and conjugated variants of the macromolecules.