Mechanism of Microbial DNA Hypervariation through Mutagenic Transposition

这项提案要求为一台既适用于这两种设备的高效液相色谱仪(Hplc)提供资金 分析和准备工作，作为支助父母赠款的主要工具补充 R01GM125669，通过突变转座实现微生物DNA超变的机制。高口径高效液相色谱法是几乎所有尖端生物化学的关键标准实验室设备。 研究。该仪器将用于化学改性的制备能力的提纯 寡核苷酸(如染料标记的引物和定点修饰的DGR基因 中间体)、蛋白质(如来自Bordatella噬菌体和来自系统发育相关的BRT和bAvd 噬菌体)和特殊的小分子(如修饰的核苷酸类似物)。我们还将使用 用于定量评估反应产物化学纯度的分析能力仪器 包括小分子、大分子和大分子的共轭变体。

This proposal requests funds for a high-performance liquid chromatograph (HPLC) that is suitable for both analytical and preparative work, as a major instrumentation supplement in support of the parent grant R01GM125669, Mechanism of Microbial DNA Hypervariation through Mutagenic Transposition. A highcaliber HPLC is a critical piece of standard laboratory equipment for almost any cutting edge biochemical research. This instrument will be used in preparative capacity for purification of chemically-modified oligonucleotides (such as dye-labeled primers and site-specifically modified DGR cDNA intermediates), proteins (such as bRT and bAvd from Bordatella phage and from phylogenetically related phage), and specialty small molecules (such as modified nucleotide analogs). We would also use the instrument in analytical capacity for quantitative assessment of chemical purity of reaction products involving small molecules, macromolecules, and conjugated variants of the macromolecules.