Membrane-associated DNA transport machines in Bacillus subtilis

摘要 在染色体分离完成之前形成分裂隔膜的细菌细胞面临着一个新的挑战。 完成DNA分离过程的非凡拓扑和机械挑战 成功地细胞分裂膜，一种DNA转运膜蛋白的复合物， 大的圆形染色体基质必须一起合作才能成功地分选， 分离复制的染色体。研究了细菌细胞分裂的关键成分 几十年来，染色体的结构和功能的基本特征 分离机制尚未探索。该项目的长期目标是结合各种 生物化学结构-功能研究与实时，活细胞成像是了解分子 染色体DNA如何成功地穿过细胞分裂隔膜的机械术语。 DNA底物通过分离机制的路径是什么？这些综合体是如何 共同发挥作用，将ATP水解循环与DNA运动结合起来？当通告的结尾 当到达染色体时，DNA的环是如何在分裂平面上分解的？做这些 蛋白质真正的功能，在孤立的DNA易位活动，或有额外的细胞 所涉及的组件？这些领域的进展将产生一个全面的模型， 分子结构和相关的生化事件参与这一DNA分离过程。

Abstract Bacterial cells that form a division septum prior to completion of chromosome segregation face an extraordinary topological and mechanistic challenge to complete the DNA segregation process successfully. The cell division membranes, a complex of DNA-translocating membrane proteins, and the large, circular chromosome substrates all must come together in concert to successfully sort and separate the replicated chromosomes. Critical components of bacterial cell division have been studied for several decades, and yet fundamental features of the structure and function of the chromosome segregation machinery remain unexplored. The long-term goal of this project which combines a variety of biochemical structure-function studies with real-time, live-cell imaging is to understand in molecular mechanistic terms how chromosomal DNA is successfully partitioned across a cellular division septum. What is the path of the DNA substrate through the segregation machinery? How do the complexes function together to couple the ATP hydrolysis cycle with DNA movement? When the end of the circular chromosome is reached, how are the loops of DNA resolved across the division plane? Do these proteins truly function in isolation for their DNA translocation activity, or are there additional cellular components that are involved? Advances in these areas will produce a comprehensive model of the molecular architecture and associated biochemical events involved in this DNA segregation process.