High-Throughput Screening and Computational Chemistry Core

项目摘要/摘要：高吞吐量筛选和计算化学核心（核心1） 高吞吐量筛选和计算化学核心（HTSCC）的目标是促进 内布拉斯加州分子靶标发现和开发中心（MTDD）的使命， 关键功能基础设施、试剂、仪器、技术专长和培训， MTDD调查人员进行他们的研究项目。通过进行高通量siRNA，化学， 虚拟计算机筛选实验，研究人员有一个新的和强大的工具，快速，有效地 产生关于基因通路和功能的准确信息，以及识别小分子 抑制剂，可以作为可逆的化学探针来剖析这些信号通路，沿着 确定药物发现的先导化合物的可能性。这些活动一般不实用， 由于成本或缺乏专门技术技能和/或 设备.对于有兴趣使用HTS和高内容成像（HCI）服务的MTDD调查人员， 能够增加自动化、简化分析并整合专为高通量设计的仪器 可大大降低试剂、塑料器皿和人工成本，适用于各种筛选和非筛选， 在他们的实验室里进行实验。另一个好处是，调查人员的范围 实验可以大大拓宽，并以更高的精度进行，通常提高了 数据的质量和再现性。在整个过程中，现场工作人员提供专业知识、培训、支持， 以及筛选技术性能方面的反馈，这些技术将最大限度地降低成本，提高产量， 最大限度地产生可解释的结果。此外，核心将不断发展和实施 新技术，并努力获得和/或升级必要的仪器。通过这些手段， 该核心将有助于使MTDD研究人员在获得研究资金方面更具竞争力，并促进长期的 长期目标是进行研究，最终改善患者的健康状况。

Project Summary/Abstract: High-Throughput Screening and Computational Chemistry Core (Core 1) The objective of the High-Throughput Screening and Computational Chemistry Core (HTSCC) is to contribute to the mission of the Nebraska Center for Molecular Target Discovery and Development (MTDD) by providing the critical functional infrastructure, reagents, instrumentation, technical expertise, and training necessary for MTDD investigators to conduct their research projects. By conducting high-throughput siRNA, chemical, and virtual in silico screening experiments, investigators have a novel and powerful tool to rapidly and efficiently generate accurate information about gene pathways and functions, as well as to identify small molecule inhibitors that can serve as reversible chemical probes to dissect these signaling pathways, along with the possibility of identifying lead compounds for drug discovery. These activities are generally not practical for the individual research laboratory to carry out for reasons of cost or lack of specialized technical skills and/or equipment. For MTDD investigators interested in using HTS and high-content imaging (HCI) services, the ability to add automation, miniaturize assays, and incorporate instrumentation designed for high-throughput can greatly reduce reagent, plastic-ware, and labor costs for a vast array of screening and non-screening experimentation performed in their laboratories. An additional benefit is that the scope of investigator experimentation can be greatly broadened and performed with greater precision, typically enhancing the quality and reproducibility of data. Throughout this process, on-site staff provides expertise, training, support, and feedback in the performance of screening technologies that will minimize costs, enhance throughput, and maximize the generation of interpretable results. Moreover, the core will continually develop and implement new technologies as needed and work to obtain and/or upgrade necessary instrumentation. By these means, the core will help to make MTDD investigators more competitive for research funding and to facilitate the long- term goal of performing research that ultimately results in the improvement of the health of patients.