Molecular Biology/Molecular Genetics (Core C)

分子生物学/分子遗传学（核心 C）

• 批准号: 10392468
• 负责人: Wendy K Chung
• 金额: $ 20.77万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10392468
• 关键词: ATAC-seq; Animal Model; Area; Basic Science; Bioinformatics; Biological; Biomedical Research; Brain; CRISPR/Cas technology; Cell Lineage; Cell model; Cells; Clinical Research; Cloning; Collaborations; Communities; Consultations; Core Facility; Coupling; DNA; DNA sequencing; Data; Data Analyses; Derivation procedure; Development; Emerging Technologies; Equipment; Faculty; Fibroblasts; Fostering; Funding; Gene Expression; Generations; Genes; Genetic Techniques; Genomics; Genotype; Goals; Grant; Human Resources; Hypothalamic structure; Infrastructure; Laboratories; Libraries; Manuscripts; Mediation; Medical; Medical center; Medicine; Methods; Modernization; Molecular; Molecular Biology; Molecular Biology Techniques; Molecular Genetics; Mus; Mutation; Neurons; Neurosciences; New York; Obesity; Pathogenesis; Patients; Peer Review; Phenotype; Phylogenetic Analysis; Physiological; Play; Preparation; Price; Process; Productivity; Publications; Reagent; Recording of previous events; Referral and Consultation; Research; Research Activity; Research Design; Research Personnel; Research Project Grants; Research Support; Resources; Ribosomal RNA; Role; Sampling; Services; Site; Students; Techniques; Technology; Time; Tissues; Training; Transgenic Mice; Universities; Viral; analytical tool; brain research; cell type; college; comorbidity; cost; design; designer receptors exclusively activated by designer drugs; differentiation protocol; experience; experimental study; feeding; genetic manipulation; genome editing; induced pluripotent stem cell; induced pluripotent stem cell technology; instrumentation; interest; investigator training; leptin receptor; member; new technology; novel; nutrition; optogenetics; programs; research and development; single cell sequencing; skills; stem cells; success; tool; transcriptome sequencing

MBMG Core PROJECT SUMMARY/ABSTRACT The Molecular Biology/Molecular Genetics (MBMG) Core has served the NYNORC obesity research community for the past 5 cycles of this grant. The MBMG Core provides critical research support for obesity-related clinical and basic research activities in the area of molecular genetics, bioinformatics, and model organism characterization. The services are widely used and have increased the productivity of NYNORC investigators, while curtailing costs and facilitating access to advanced genomics technologies. The primary objective of this Core is to assist investigators to apply the tools and technologies of molecular genetics and genomics to elucidate the molecular-genetic basis for the pathogenesis and medical/physiological co-morbidities of obesity. The Specific Aims of the MBMG Core are: 1) To facilitate the application of molecular genetics to problems of obesity by providing expert consultation on both study design and applicable molecular biological techniques; 2) To provide standard laboratory services to young investigators and those without formal labs for studies related to obesity; 3) To develop and make available new cutting edge research tools and reagents. To these ends, the MBMG Core offers consultation and services for molecular methods including gene expression, DNA extraction, genotyping, DNA and RNA sequencing, bacterial phylogenetic characterization by 16s rRNA, RNAscope, clearing of tissue by SHIELD reagents, mouse transgenics, stem cell derivation and differentiation, and genome editing with CRISPR Cas9, and analytic tools related to these techniques. Core services are of 3 basic types: (i) On-site Direct Services, (ii) Consultative-Collaborative services with Institutional Cores, (iii) Consultative- Referral. To gain access to cutting-edge technologies, avoid duplication and to maximally leverage our resources, we collaborate extensively with other local Core facilities and laboratories. Core personnel provide assistance ranging from study design to execution of studies and data interpretation. We emphasize service to young investigators and P&F grantees. The Core has been active in developing new research tools and reagents and has played an essential role in establishing iPS cell technology and optogenetics for our community. Our R&D activities are focused on developing new tools and reagents for brain research and genetic manipulations in cells and model animals. The MBMG Core has been a nexus for intellectual exchange and collaboration, and an important training venue for students, fellows and junior faculty. During the past cycle, the Core processed over >20,000 samples from 39 NORC members supported by 43 grants, in addition to 10 non-members. These services helped investigators generate 68 new grants and 107 publications.

MBMG核心 项目概要/摘要 分子生物学/分子遗传学 (MBMG) 核心服务于 NYNORC 肥胖研究界 过去 5 个周期的补助金。 MBMG 核心为肥胖相关临床提供关键研究支持 分子遗传学、生物信息学和模式生物领域的基础研究活动 表征。这些服务得到广泛使用，提高了 NYNORC 调查人员的工作效率， 同时降低成本并促进先进基因组学技术的获取。此举的首要目标 核心是协助研究者应用分子遗传学和基因组学的工具和技术 阐明肥胖发病机制和医学/生理共病的分子遗传学基础。 MBMG 核心的具体目标是： 1) 促进分子遗传学在以下问题上的应用： 肥胖症，提供有关研究设计和适用分子生物学技术的专家咨询； 2） 为年轻研究者和没有正规实验室进行相关研究的人员提供标准实验室服务 肥胖； 3）开发并提供新的尖端研究工具和试剂。为此， MBMG Core 提供分子方法的咨询和服务，包括基因表达、DNA 提取、 基因分型、DNA 和 RNA 测序、通过 16s rRNA、RNAscope 进行细菌系统发育表征， 通过 SHIELD 试剂、小鼠转基因、干细胞衍生和分化以及基因组清除组织 使用 CRISPR Cas9 进行编辑，以及与这些技术相关的分析工具。核心服务有 3 种基本类型：(i) 现场直接服务，(ii) 与机构核心的咨询-合作服务，(iii) 咨询- 推荐。为了获得尖端技术、避免重复并最大限度地利用我们的优势 资源，我们与其他当地核心设施和实验室广泛合作。核心人员提供 协助范围从研究设计到研究执行和数据解释。我们强调服务于 年轻研究者和 P&F 受资助者。核心一直积极开发新的研究工具和试剂 并在为我们社区建立 iPS 细胞技术和光遗传学方面发挥了重要作用。我们的 研发活动的重点是开发用于大脑研究和基因操作的新工具和试剂 在细胞和模型动物中。 MBMG 核心一直是知识交流与协作的纽带，并且 学生、研究员和初级教师的重要培训场所。在过去的周期中，核心处理了 来自 39 个 NORC 成员的超过 20,000 个样本，除 10 个非成员外，还得到 43 项资助的支持。 这些服务帮助研究人员获得了 68 项新资助和 107 篇出版物。