Viral Vector

病毒载体核心（VVC）是一个支持基因转移研究的研究机构。其整体 目的是支持研究人员使用基因转移技术，包括咨询 研究人员，开发新的载体，对功能产生的载体进行合作测试， 纯度和制备，包括质量控制。该设施既为HCCC成员服务， 来自其他NCI-designated癌症中心的研究者人数。VVC工作人员积极参与 癌症领域基因转移技术的发展。与多名研究者的互动 来自不同学科和机构允许思想，技术进步和 矢量设计的创新。VVC提供了重组蛋白的纯化和浓缩制剂， 腺病毒、腺相关病毒、牛痘、杆状病毒和逆转录病毒（包括慢病毒）。它还 提供了标准细胞系、表达质粒和重组报告病毒的储备 向量。VVC的主要职责是：制备重组载体;进行相关质量 控制;传播病媒;维持可供使用的病媒种群数据库;维持数据库 表达载体;根据需要开发新的表达载体;开发用于载体的新方法 协助设计和开发新的载体。

The Viral Vector Core (VVC) serves as a research facility that supports gene transfer studies. Its overall objective is to support investigators in the use of gene transfer technologies, including consultation with investigators, development of novel vectors, collaborative testing of vectors generated for function and purity, and preparation including quality control. The facility serves both HCCC members and a significant number of investigators from other NCI-designated cancer centers. VVC staff are active participants in the development of gene transfer technologies in the cancer field. The interaction with multiple investigators from various disciplines and institutions allows for cross-fertilization of ideas, technical advancements, and innovations in vector designs. The VVC provides purified and concentrated preparations of recombinant adenovirus, adeno-associated virus, vaccinia, baculovirus and retrovirus (including lentivirus). It also provides access to standard cell lines, expression plasmids and a stock of recombinant reporter viral vectors. The main responsibilities of the VVC are to: prepare recombinant vectors; perform relevant quality control; disseminate vectors; maintain a database of vector stocks available for use; maintain a database of expression vectors; develop new expression vectors as needed; develop novel methods for vector production; and assist in the design and development of novel vectors.