Core B: Gene Transfer Vector Core

项目总结/摘要 爱荷华州大学的载体核心被整合到基因治疗项目中，旨在研究 肺部疾病。Vector Core的工作人员积极参与基因转移的发展 囊性纤维化中心以及神经生物学、心血管、癌症和黄斑的技术 退化中心。与来自不同学科的研究人员的互动允许交叉施肥， 创意、技术进步和创新。Vector Core的总体目标是 支持CF相关基因转移技术的研究者。这包括咨询、发展 新型载体、载体的功能和纯度的协作测试以及常规载体制备。核心工作人员 和研究人员在设计和生成的所有阶段都保持密切联系。核心将提供 重组腺病毒、腺相关病毒（AAV）和 慢病毒载体核心还将提供标准细胞系、表达质粒和 报告病毒。因此，核心作为基因转移研究的研究和开发设施， 常规病媒制剂的服务设施。Vector Core还拥有一个非常强大的民族 通过将病毒载体分发给爱荷华州以外的许多机构和公司，

PROJECT SUMMARY/ABSTRACT The Vector Core at the University of Iowa is integrated into gene therapy projects directed at the study of diseases of the lung. The Vector Core staff are active participants in the development of gene transfer technologies for the Cystic Fibrosis Center as well as Neurobiology, Cardiovascular, Cancer, and Macular Degeneration Centers. The interaction with investigators from various disciplines allows for cross-fertilization of ideas, technical advancements, and innovations in vector design. The overall objective of the Vector Core is to support investigators in gene transfer technologies relevant to CF. This includes consultation, development of novel vectors, collaborative testing of vectors for function and purity, and routine vector preparations. Core staff and investigators are in close contact through all phases of design and generation. The Core will provide purified and concentrated preparations of recombinant adenovirus, adeno-associated virus (AAV), and lentivirus. The Vector Core will also provide access to standard cell lines, expression plasmids, and stocks of reporter viruses. Thus, the Core serves as a research and development facility for gene transfer studies, and a service facility for routine vector preparations. The Vector Core also has an exceptionally strong national presence by distributing viral vectors to many institutions and companies outside of Iowa.