Viral Vector

病毒载体核心(VVC)作为支持基因转移研究的研究设施。它的整体 目的是支持研究人员使用基因转移技术，包括咨询 调查人员，开发新的载体，协作测试为函数和 纯度和制备，包括质量控制。该设施为HCCC成员和一个重要的 来自NCI指定的其他癌症中心的研究人员数量。VVC员工积极参与 基因转移技术在癌症领域的发展。与多名调查人员的互动 来自不同的学科和机构，允许思想、技术进步和 矢量设计的创新。VVC提供重组人的纯化和浓缩制剂 腺病毒、腺相关病毒、牛痘病毒、杆状病毒和逆转录病毒(包括慢病毒)。它还 提供获得标准细胞系、表达质粒和重组报告病毒的途径 向量。VVC的主要职责是：准备重组载体；执行相关质量 控制；传播媒介；维护可供使用的媒介种群数据库；维护数据库 表达载体；根据需要开发新的表达载体；开发新的载体方法 生产；并协助设计和开发新的载体。

The Viral Vector Core (VVC) serves as a research facility that supports gene transfer studies. Its overall objective is to support investigators in the use of gene transfer technologies, including consultation with investigators, development of novel vectors, collaborative testing of vectors generated for function and purity, and preparation including quality control. The facility serves both HCCC members and a significant number of investigators from other NCI-designated cancer centers. VVC staff are active participants in the development of gene transfer technologies in the cancer field. The interaction with multiple investigators from various disciplines and institutions allows for cross-fertilization of ideas, technical advancements, and innovations in vector designs. The VVC provides purified and concentrated preparations of recombinant adenovirus, adeno-associated virus, vaccinia, baculovirus and retrovirus (including lentivirus). It also provides access to standard cell lines, expression plasmids and a stock of recombinant reporter viral vectors. The main responsibilities of the VVC are to: prepare recombinant vectors; perform relevant quality control; disseminate vectors; maintain a database of vector stocks available for use; maintain a database of expression vectors; develop new expression vectors as needed; develop novel methods for vector production; and assist in the design and development of novel vectors.