The Cleveland Digestive Diseases Research Core Center (DDRCC)

次级核心摘要 克利夫兰DDRCC鼠标模型核心的目标是提供一套全面的成本效益， 为DDRCC研究者提供服务和共享资源，以增强其体内研究的性能 主要研究消化道炎症/肿瘤发生和肝脏疾病/代谢的发病机制。的 该核心的总体目标是提供集中的小鼠建模服务，这将提高体内 凯斯西储大学和克利夫兰诊所Lerner的消化系统疾病相关研究 研究机构和提供公共的，具有成本效益的访问独特的小鼠模型的肠道 炎症和肝脏疾病，从而产生新的研究项目，资助机会和合作 在这些调查领域。支持无菌（GF）和粪便材料的开发 移植（FMT）子核心，我们正在请求行政补充，以扩大这一子核心。我们 提出了以下三个具体目标：1）提供GF SAM P1/YitFc，温妮， 和TNFΔARE小鼠进行实验;（2）进行肠道微生物组的粪便材料移植（FMT） 转化为GF小鼠;和（3）将独特的小鼠品系及其衍生物再衍生为GF小鼠。因此，GF和 FMT次核心通过提供独特的可用资源和成本组合使复员方案协调委员会成员受益， 有效的服务，促进在克利夫兰有关消化系统疾病的研究的发展 科学界和其他领域。

SUB-CORE SUMMARY The objective of the Cleveland DDRCC Mouse Models Core is to provide a comprehensive set of cost-effective services and shared resources to DDRCC investigators that augments their performance of in vivo studies focused on the pathogenesis of Digestive Inflammation/Tumorigenesis and Liver Disease/Metabolism. The overall goal of this Core is to provide centralized mouse modeling services that will enhance the pace of in vivo digestive disease-related research at Case Western Reserve University and the Cleveland Clinic Lerner Research Institute and provide communal, cost-effective access to unique mouse models on intestinal inflammation and liver disease, thus resulting in new research projects, funding opportunities, and collaborations in these areas of investigation. In support of the development of a germ-free (GF) and fecal material transplantation (FMT) sub-core, we are requesting an administrative supplement to expand this sub-core. We are proposing the following three Specific Aims: 1) Provide experimental cohorts of GF SAMP1/YitFc, Winnie, and TNFΔARE mice for experimentation; (2) performing fecal material transplantation (FMT) of gut microbiome into GF Mice; and (3) rederivation of unique mouse strains and their derivatives into GF mice. Thus, the GF and FMT sub-core benefits DDRCC members by providing a combination of uniquely available resources and cost- effective services that facilitate the development of research related to digestive diseases in the Cleveland scientific community and beyond.