Muscle clock and weakness: diversity supplement
Muscle clock and weakness: diversity supplement
批准号:
10414186
负责人:
Karyn A Esser
金额:
$3.73万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
未结题
起止时间:
2021-05-29 至 2026-02-28
关键词:
3-DimensionalARNTL geneATAC-seqAdministrative SupplementAgeAgingAntibodiesAttentionBioluminescenceChIP-seqChromatinCircadian RhythmsCoupledDNA BindingDataDoseElementsFemaleFloridaGene ExpressionGenesGeneticGenomicsHealthKnockout MiceLearningLengthLongevityMeasuresMedicineMentorsMicroscopyMissionMolecularMolecular TargetMuscleMuscle WeaknessMuscle functionOutcomeOutputPhenotypePhysiologicalPopulation HeterogeneityPropertyProteinsRNA SplicingRegulationResearchResolutionRodent ModelRoleSarcomeresScientistSeriesSkeletal MuscleStructureStudentsTechniquesTestingThick FilamentTissuesTrainingTraining ActivityUnited States National Institutes of HealthUniversitiesWorkadeno-associated viral vectorbone imagingcareercareer developmentcircadian pacemakercollegeconfocal imagingdoctoral studentepigenomicsexperienceexperimental studyfallsfunctional outcomesgraduate studenthuman subjectimaging approachimprovedinterestmalemetabolomicsmouse modelmuscle strengthmuscular structureparent grantparent projectpre-clinicalresearch and developmentresponseskillstranscriptome sequencingtranscriptomics
中文摘要
摘要:本项目旨在为Silvana提供研究和职业发展培训
英文摘要
Abstract: The purpose of this project is to provide research and career development training for Silvana
Sidhom, a current PhD student at the University of Florida. This administrative supplement in response to PA-
21-071: “Research Supplements to Promote Diversity in Health-Related Research (Admin Supp)” will facilitate
career development for the candidate and as well as contribute to the mission of the parent project – to define
molecular targets downstream of the circadian clock that modulate muscle structure with implications for
strength.
The primary objectives of Ms. Sidhom’s proposed research will be to work on experiments related to Aim 2 of
the parent grant. First, she will learn to use confocal imaging approaches with sarcomeric antibodies to define
changes in the M-line and Z-line structural elements of the sarcomere from the control and muscle specific
Bmal1 KO mice. Second, she will use AAV approaches to restore expression of selected sarcomeric genes,
such as Tcap, to test if that is sufficient to restore structure and muscle force. Ms. Sidhom will work actively
with Collin Douglas, another PhD student working on the parent grant as well as Dr. Christopher Wolff, a
postdoctoral associate on this project. Dr. Esser, the PI of the R01, will be the primary mentor for Ms. Sidhom
within the College of Medicine at the University of Florida. We have established her committee and this
includes: Drs. Russ Hepple, Christiaan Leeuwenburgh and Richard Dunn. They are enthusiastic about Ms.
Sidhom as a student and provide appropriate expertise in skeletal muscle and imaging approaches. Silvana
has completed her first year of required courses so the committee will work with Ms. Sidhom to outlined a
series of didactic courses, including the skeletal muscle course taught each fall. The committee will also help
guide Silvana with experiential training activities that will equip her with the necessary skills to progress as an
independent scientist and to fulfill the NIH mission to advance the careers of a diverse population of scientists.
The project is directly related to Aim 2 of the parent grant:
Specific Aim 2: To test the clock controlled genes, Rbm20 and/or Tcap, for their roles in sarcomere
structure and muscle function. This aim will use AAV delivery of Tcap and/or Rbm20 in Bmal1 deficient
muscle to test their contribution to sarcomere structure, myofibrillar protein composition and muscle function.
Sarcomere structural elements will be obtained by super-resolution microscopy with deconvolution techniques
with antibodies to define 2D and 3D properties including sarcomere length, thick filament centrality, M-band
and Z line integrity. Deep RNA-sequencing will be performed on the Rbm20 muscles to define splicing
changes. Functional outcomes will include ex vivo measures to test the impact of structural changes on both
passive/stiffness and active/max force and rate of contraction properties.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
MoTrPAC Supplemental Funding for Miller
-
批准号:10889549
-
项目类别:
-
资助金额:$6.51万
-
财政年份:2023
-
负责人:Karyn A Esser
-
依托单位:
Circadian Clock and Muscle Health
-
批准号:10372227
-
项目类别:
-
资助金额:$52.98万
-
财政年份:2021
-
负责人:Karyn A Esser
-
依托单位:
Circadian Clock and Muscle Health
-
批准号:10583484
-
项目类别:
-
资助金额:$54.35万
-
财政年份:2021
-
负责人:Karyn A Esser
-
依托单位:
Circadian Clock and Muscle Health
-
批准号:10796287
-
项目类别:
-
资助金额:$7.46万
-
财政年份:2021
-
负责人:Karyn A Esser
-
依托单位:
Circadian Clock and Muscle Health
-
批准号:10581006
-
项目类别:
-
资助金额:$7.46万
-
财政年份:2021
-
负责人:Karyn A Esser
-
依托单位:
Molecular Transducers of Physical Activity Consortium Coordinating Center
-
批准号:10840609
-
项目类别:
-
资助金额:$310.0万
-
财政年份:2017
-
负责人:Karyn A Esser
-
依托单位:
MoTrPAC Consortium Coordinating Center
-
批准号:10322175
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2016
-
负责人:Karyn A Esser
-
依托单位:
UF PASS: Regulation of exercise transducers
-
批准号:10341087
-
项目类别:
-
资助金额:$43.48万
-
财政年份:2016
-
负责人:Karyn A Esser
-
依托单位:
UF PASS: Regulation of exercise transducers
-
批准号:9515180
-
项目类别:
-
资助金额:$14.2万
-
财政年份:2016
-
负责人:Karyn A Esser
-
依托单位:
UF PASS Administrative Supplement: Regulation of exercise transducers
-
批准号:10746522
-
项目类别:
-
资助金额:$34.91万
-
财政年份:2016
-
负责人:Karyn A Esser
-
依托单位:
Muscle weakness and neurogeneration; exercise as a therapeutic approach
-
批准号:10287587
-
项目类别:
-
资助金额:$37.43万
-
财政年份:2016
-
负责人:Karyn A Esser
-
依托单位:
UF PASS: Regulation of exercise transducers
-
批准号:10533648
-
项目类别:
-
资助金额:$6.61万
-
财政年份:2016
-
负责人:Karyn A Esser
-
依托单位:
UF PASS: Regulation of exercise transducers
-
批准号:9246861
-
项目类别:
-
资助金额:$2.25万
-
财政年份:2016
-
负责人:Karyn A Esser
-
依托单位:
UF PASS: Regulation of exercise transducers: supplement for diverse students
-
批准号:10372539
-
项目类别:
-
资助金额:$3.46万
-
财政年份:2016
-
负责人:Karyn A Esser
-
依托单位:
Molecular clock and skeletal muscle weakness
-
批准号:8674218
-
项目类别:
-
资助金额:$32.96万
-
财政年份:2014
-
负责人:Karyn A Esser
-
依托单位:
Intelligent and Automatic Image Segmentation Software for High ThroughputAnalysi
-
批准号:8699686
-
项目类别:
-
资助金额:$14.28万
-
财政年份:2013
-
负责人:Karyn A Esser
-
依托单位:
Intelligent and Automatic Image Segmentation Software for High ThroughputAnalysi
-
批准号:8522756
-
项目类别:
-
资助金额:$15.0万
-
财政年份:2013
-
负责人:Karyn A Esser
-
依托单位:
BetaCatenin Regulation of Skeletal Muscle Hypertrophy
-
批准号:8320556
-
项目类别:
-
资助金额:$33.41万
-
财政年份:2012
-
负责人:Karyn A Esser
-
依托单位:
Beta Catenin Regulation of Skeletal Muscle Hypertrophy
-
批准号:8582361
-
项目类别:
-
资助金额:$2.03万
-
财政年份:2012
-
负责人:Karyn A Esser
-
依托单位:
BetaCatenin Regulation of Skeletal Muscle Hypertrophy
-
批准号:8500216
-
项目类别:
-
资助金额:$31.74万
-
财政年份:2012
-
负责人:Karyn A Esser
-
依托单位: