UF PASS: Regulation of exercise transducers

UF PASS：运动传感器的调节

• 批准号: 9515180
• 负责人: Karyn A Esser
• 金额: $ 14.2万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-12-15 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9515180
• 关键词: Acute; Age; Animal Model; Animals; Apoptosis; Autophagocytosis; Blood; Body Composition; Body Weight; Brain; Cells; Chemicals; Chronic Disease; Collection; Custom; Darkness; Data; Data Collection; Databases; Dependovirus; Disease; Exercise; Female; Florida; Frequencies; Genes; Genetic screening method; Goals; Guidelines; Harvest; Health; Home environment; Human; Informatics; Ischemia; Kidney; Length; Libraries; Location; Maps; Methods; Modeling; Molecular; Monitor; Muscle; Muscle Cells; Neurons; Outcome; Oxidative Stress; Phase; Physical activity; Physiological; Protocols documentation; Quality Control; Rattus; Rattus norvegicus; Regulation; Reperfusion Therapy; Reporter; Running; Sampling; Scientist; Sepsis; Ships; Site; Skeletal Muscle; Small Interfering RNA; Stimulus; Stress; System; Techniques; Technology; Testing; Therapeutic; Time; Tissue Banks; Tissues; Training; Training Programs; Transducers; United States National Institutes of Health; Universities; VO2max; Work; base; cohort; endoplasmic reticulum stress; endurance exercise; experimental study; expression vector; gain of function; high throughput screening; improved; in vivo; kidney cell; male; novel; overexpression; oxidant stress; phase 1 study; phase 2 study; physical model; pre-clinical; preconditioning; prevent; release factor; resistance exercise; response; screening; secondary outcome; strength training; stress tolerance; treadmill

Abstract Exercise is a powerful and pleiotropic physiological stimulus that helps prevent many chronic diseases and is used as a therapeutic for disease. While the beneficial effects of exercise are extensively acknowledged there is still very little understood about the molecular transducers of the systems-wide effects. The goal of this University of Florida Molecular Transducers of Physical Activity Preclinical Animal Study Sites application (UF PASS) is to conduct experiments in animals that will provide tissues/blood (i.e. biospecimens) to the Chemical Analysis Sites for identification of molecular transducers induced by defined models of physical activity from tissues that cannot be obtained from humans as well as to conduct mechanistic studies that can support screening of novel transducers to quickly move the field forward. In Phase 1, UF PASS proposes to collect biospecimens for the Chemical Analysis sites following endurance (run-training) or resistance exercise protocols on male and female Fischer 344xBrown Norway rats (F344-BN) at three different ages. To better capture the dynamics of the exercise/adaptation responses we propose to: 1) Collect biospecimens at 5 selected timepoints following an acute bout of exercise on naïve and trained rats; 2) Collect biospecimens following short duration training (after 5 bouts) and 3) Collect biospecimens following long-term (8 weeks) training. For Phase 2, our hypothesis is that factors released from muscle (i.e. myokines) are the molecular transducers that function throughout the system to improve the well-established stress tolerance. The goal of these studies will be to employ high throughput screening technologies to test up to 1500 myokines. We will then use secondary screening techniques to test 100 candidates from which we will select up to 3 candidates for in vivo testing. The results of the experiments in Aim 3 will provide molecular evidence identifying a set of transducers, released from muscle, that are necessary for exercise induced systemic health. The goals of the UF PASS will be pursued by the following Specific Aims: Specific Aim 1: Center Coordination Phase. Specific Aim 2: Phase 1 Studies. To perform endurance and resistance exercise using male and female F344BN rats at 3-4, 16-18, and 27-29 mo. Specific Aim 3: Phase 2 Studies. The goal in Aim 3 is to test myokines as the exercise transducers for improved stress tolerance.

运动是一种强有力的多效性生理刺激，有助于预防许多慢性疾病