A single cell spatial genomics platform for multi-modal characterization of tissue organization

项目摘要/摘要 细胞是组织和器官的单位构建块，因此，要了解组织如何 为了了解细胞的功能，了解不同类型的细胞在健康和疾病中的位置是重要的。 在这个提案中，我们建议开发一个新的空间基因组学平台，该平台将使 在单细胞水平上的组织结构的多模式表征。这将是 革命性地研究发育和病理变化中的组织组织 在衰老和疾病方面。我们的工具将使我们能够查看RNA、蛋白质和调节元件 在DNA中，所有的都是在组织的背景下。我们将确保这些新的基因组工具易于使用 和采用，这将是特别强大的病理环境，那里的组织资源 是珍贵的，工具的采用受到易用性的限制。圆满完成 这项工作将导致一个全面的工具集，以了解组织结构 单细胞组织的视角。

Project Summary/Abstract Cells are the unit building blocks of tissues and organs, thus, to understand how tissues function, it is important to understand where different types of cells reside in health and disease. In this proposal, we propose to develop a novel spatial genomics platform that will enable multi-modal characterization of tissue structure at the single cell level. This will be revolutionary for the study of tissue organization in development and pathological changes in aging and disease. Our tools will enable us to look at RNA, proteins and regulatory elements in DNA all within the tissue context. We will ensure these novel genomic tools are easy to use and adopt, which will be especially powerful for pathological settings, where tissue resources are precious, and adoption of tools is limited by ease of use. The successful completion of this work will lead to a comprehensive toolset for understanding tissue structure from the perspective of single cell organization.