A single cell spatial genomics platform for multi-modal characterization of tissue organization

用于组织组织多模式表征的单细胞空间基因组学平台

• 批准号: 9797023
• 负责人: Fei Chen
• 金额: $ 94.64万
• 依托单位: BROAD INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-08 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9797023
• 关键词: ATAC-seq; Adopted; Adoption; Aging; Algorithms; Biological Assay; Brain; Cells; Chromatin; Chromosomes; Complex; DNA; Data; Data Analyses; Data Collection; Data Set; Development; Disease; Ensure; Epigenetic Process; Gene Expression Profile; Generations; Genes; Genomic approach; Health; Individual; Joints; Libraries; Location; Measurement; Measures; Messenger RNA; Miniaturization; Molecular; Molecular Biology; Organ; Pathologic; Process; Proteins; Protocols documentation; RNA; Regulatory Element; Research Personnel; Resolution; Slide; Structure; Technology; Testing; Tissues; Transposase; Validation; Work; antibody libraries; cell type; computerized tools; data integration; developmental disease; experimental study; genomic platform; genomic tools; high throughput technology; indexing; multimodal data; multimodality; new technology; next generation; novel; oligo (dT); protein expression; single cell sequencing; single-cell RNA sequencing; tissue resource; tool; transcriptome; transcriptome sequencing; transcriptomics

Project Summary/Abstract Cells are the unit building blocks of tissues and organs, thus, to understand how tissues function, it is important to understand where different types of cells reside in health and disease. In this proposal, we propose to develop a novel spatial genomics platform that will enable multi-modal characterization of tissue structure at the single cell level. This will be revolutionary for the study of tissue organization in development and pathological changes in aging and disease. Our tools will enable us to look at RNA, proteins and regulatory elements in DNA all within the tissue context. We will ensure these novel genomic tools are easy to use and adopt, which will be especially powerful for pathological settings, where tissue resources are precious, and adoption of tools is limited by ease of use. The successful completion of this work will lead to a comprehensive toolset for understanding tissue structure from the perspective of single cell organization.

项目概要/摘要 细胞是组织和器官的基本组成部分，因此，了解组织如何 功能，了解不同类型的细胞在健康和疾病中所处的位置非常重要。 在本提案中，我们建议开发一个新颖的空间基因组学平台，该平台将使 单细胞水平组织结构的多模式表征。这将是 对于发育和病理变化中的组织组织研究具有革命性意义 在衰老和疾病中。我们的工具将使我们能够研究 RNA、蛋白质和调控元件 DNA 都在组织范围内。我们将确保这些新颖的基因组工具易于使用 并采用，这对于病理环境尤其有效，因为组织资源 是宝贵的，工具的采用受到易用性的限制。顺利完成 这项工作将产生一个全面的工具集，用于理解组织结构 单细胞组织的视角。