High-throughput mapping of synaptic connectivity between transcriptomically defined cell types
High-throughput mapping of synaptic connectivity between transcriptomically defined cell types
批准号:
10413540
负责人:
Michael Nicholas Economo
金额:
$142.38万
依托单位国家:
美国
项目类别:
财政年份:
2022
资助国家:
美国
项目状态:
未结题
起止时间:
2022-05-01 至 2025-04-30
关键词:
AddressAdoptedAnterolateralArchitectureBehaviorBehavioralBenchmarkingBiological AssayBrainBrain regionCellsCognitionDataDevelopmentDiseaseElectron MicroscopyElectrophysiology (science)Emerging TechnologiesFluorescenceFluorescent in Situ HybridizationGene Expression ProfileGenesGeneticGoalsGoldImageIndividualIon ChannelKnowledgeLearningLightLinkMapsMeasurementMeasuresMethodologyMethodsMicroscopyMolecularMolecular ComputationsMotorMotor CortexMovementMusNeocortexNeuronsNeurosciencesOpticsPathway interactionsPatternPerceptionPhysiologyPopulationProbabilityProcessReportingResourcesShort-Term MemoryStructureSynapsesSystemTechniquesTechnologyThalamic structureTheoretical modelTimeTissuescell typeexperienceexperimental studyimaging informaticsinsightinter-individual variationneural circuitneurophysiologynew technologynoveloptogeneticsrelating to nervous systemresponsesensorsingle cell analysissingle-cell RNA sequencingstandard measuretooltranscriptomicsvoltage
中文摘要
项目总结
英文摘要
PROJECT SUMMARY
Identifying the cell types that make up each region of the brain and the patterns of synaptic connections
through which they are linked is key to understanding how neural circuits give rise to all perception, cognition,
and behavior. Rapid improvements in optical, molecular, and computational technologies are enabling large-
scale projects aiming to comprehensively map the cell types that comprise the mammalian brain.
Nevertheless, defining the microconnectivity of the thousands of cell types in the brain remains challenging
due to a lack of scalable methods. This proposal describes the development of a technology for addressing this
methodological gap. Using a novel combination of high-sensitivity fluorescence voltage imaging and single-
neuron optogenetic photostimulation, we will map synaptic connectivity within – and between – brain regions.
Using this approach, synaptic connectivity can be mapped with throughput two to three orders of magnitude
higher than existing techniques. Importantly, leveraging an all-optical approach to mapping connectivity will
allow us integrate synaptic connectivity measurements with emerging techniques for highly multiplexed
fluorescence in situ hybridization. In this way, we can identify the molecular identities of large neuronal
populations and their connectivity. We will demonstrate the technology developed in this proposal in the
motor cortex, a region where knowledge of gene expression patterns has far outpaced our ability to identify
connectivity motifs. Revealing both local connectivity motifs and the precise molecular identity of cells that
receive long-range input from the thalamus – an important driver of cortical activity – will provide new
insights into the circuit mechanisms supporting voluntary movements.
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会议论文
Linking Motor Cortex Activity and Movement in the Mouse Orofacial System
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批准号:10554287
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项目类别:
-
资助金额:$41.25万
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财政年份:2022
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负责人:Michael Nicholas Economo
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依托单位:
Linking motor cortex activity and movement in the mouse orofacial system.
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批准号:10367341
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项目类别:
-
资助金额:$41.25万
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财政年份:2022
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负责人:Michael Nicholas Economo
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依托单位:
Linking Motor Cortex Activity and Movement in the Mouse Orofacial System
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批准号:10759697
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项目类别:
-
资助金额:$3.62万
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财政年份:2022
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负责人:Michael Nicholas Economo
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依托单位:
Linking motor cortex activity and movement in the mouse orofacial system.
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批准号:10688983
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项目类别:
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资助金额:$4.11万
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财政年份:2022
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负责人:Michael Nicholas Economo
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依托单位:
The Functional Organization of Inhibition in the Olfactory Bulb In Vivo
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批准号:8397200
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项目类别:
-
资助金额:$4.92万
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财政年份:2012
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负责人:Michael Nicholas Economo
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依托单位:
The Functional Organization of Inhibition in the Olfactory Bulb In Vivo
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批准号:8573250
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项目类别:
-
资助金额:$3.0万
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财政年份:2012
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负责人:Michael Nicholas Economo
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依托单位:
海外基金