Orbitrap ID-X Tribrid Mass Spectrometer for Unbiased Global Metabolomics Profiling

摘要

ABSTRACT The goal of this S10 application is to request funding for a high resolution Orbitrap ID-X Tribrid mass spectrometer (OT IDX) to enable identification and quantification of global metabolite profiles in various biospecimens. This will fill a “missing link” in the Metabolomics Core at Baylor College of Medicine that has to date, excelled in offering targeted metabolic profiling platforms to support scientific investigations. OT IDX will be used to develop robust and well tested methods for profiling steady state levels of metabolites in a global fashion as well as changes in metabolic flux in a global scale. These technologies that will be offered as a new line of service in the Metabolomics Core will support studies using pre-clinical specimens (cell line-derived xenografts, patient-derived xenografts and transgenic models), clinical specimens and cell lines. Importantly, the unbiased technologies will support projects in the areas of Cancer, Diabetes, Obesity, Environmental Exposures, Viral infections, etc. The OT IDX will be administered by the Baylor Metabolomics Core under the Advanced Technology Core Umbrella. The installation, and implementation of OT IDX will be significantly supported by the institutional funding that will cover personnel, Service Contract, software and training costs. The functioning and output of the OT IDX will be monitored by the Internal Advisory Committee and External Advisory Committee, which have experts in metabolomics, Core leaders and institutional representatives. Based on the outstanding track record of the Metabolomics Core at Baylor College of Medicine, the Core is strongly positioned to establish the innovative unbiased global metabolic platform using OT IDX in the institution. This will allow investigators to develop novel hypothesis and leads that could culminate in new biomarkers and novel therapeutic interventions.