Shared Resource: Metabolomics

代谢组学共享资源（组学组） 项目摘要 癌症的发展和进展长期以来一直被认为是多因素的。开始理解 从系统的角度来看癌症进展，我们需要表征和整合各种分子 分析的各种生物标本中涉及的组件，定义了系统生物学的新时代，或 OMICS。“组学”时代的出现，已经将重点从单个基因的评估， 蛋白质的表达，以检查表达的基因组和蛋白质组的实质性组成部分。破译 区分进展为晚期疾病的肿瘤亚群的分子网络将描绘出肿瘤的分子结构。 癌症的病因学，以及导致生物标志物的识别，这将有助于识别患者 需要治疗的疾病虽然这些研究已经使用全局转录组学进行，但对一些人来说， 在蛋白质组学平台的范围内，在研究全球代谢领域取得的成就要少得多。 改变。当前的应用程序旨在创建一个以癌症为中心的代谢组学共享资源， 在广泛的范围内测量代谢物水平的服务、基础设施、专业知识、支持和培训 样本使用各种正交技术平台，共同可以测量一个显着的广度 代谢组具体而言，这种共享资源将测量极地、中 极性和非极性代谢物，包括脂质，以及测量细胞系，组织， 血浆、血清和尿液。

CORE: Metabolomics Shared Resource (Omics Group) PROJECT SUMMARY Development and progression of cancer has long been known to be multifactorial. To begin to understand cancer progression with a systems perspective, we need to characterize and integrate the various molecular components involved across the various biospecimens analyzed, defining the new era of Systems Biology, or OMICS. The emergence of the “-omics” era has shifted the focus from the assessment of individual genes and proteins to examination of a substantial component of the expressed genome and proteome. Deciphering the molecular networks that distinguish subsets of tumors that progress to advanced disease will delineate the etiology of cancer, as well as lead to the identification of biomarkers that will aid in the identification of patients that should be treated. Although such studies have been carried out using global transcriptomics, and to some extent proteomics platforms, much less has been achieved in the domain of studying global metabolic alterations. The current application aims to create a cancer-centric metabolomics shared resource to provide service, infrastructure, expertise, support and training on measurement of metabolite levels in a broad range of specimens using various orthogonal technology platforms that together can measure a significant breadth of the metabolome. Specifically, this shared resource will measure qualitative and quantitative levels of polar, mid polar and non-polar metabolites including lipids as well as measure pathway activity in cell lines, tissues, plasma, serum and urine.