Studies on Antimicrobial resistance in bacteria of veterinary importance

项目概要/摘要 纽约州/康奈尔动物健康诊断中心（AHDC）是一个提供全方位服务的多学科 兽医诊断实验室，也是美国东北部唯一的一家此类实验室。AHDC 在2016年收到了超过20万客户的加入。细菌学部分有MALDI-TOF、灵敏度、血液 培养机，BSL 3可拆卸工作空间和16 S DNA测序功能，使其成为全方位服务 细菌学实验室此外，AHDC拥有内部ISO批准的全基因组测序 这是传染病实验室独有的能力。AHDC细菌学实验室 2016年对临床样本、组织、血清、细菌分离株进行了约10万次检测， 环境、食品和饲料样品。AHDC与FDA合作，在以下方面发挥了积极作用： 宠物食品和动物饲料污染调查多次在过去十几年。我们希望 继续参与FDA Vet-LIRN计划和其他网络实验室，以： 1)继续参与VPO指定的样本分析和监测活动， 动物健康和福利，并增加Vet-LIRN网络的激增能力，以协助紧急情况 和大规模爆发测试。 2)通过制定和使用标准化方法，提供分析数据，以支持监管行动， 设备平台和报告方法，继续参与能力验证提供的 VPO，继续按照VPO的要求调查消费者报告的案件，并继续 按照VPO的要求，改进和实施标准化的质量管理体系。 3)继续参与小规模的抗生素敏感性试验研究，以解决新出现的 抗生素耐药性问题，并参与全基因组测序计划。 AHDC的细菌学部门打算继续与FDA Vet-LIRN、Vet- LIRN网络，以及联邦、州、地方和部落组织，以促进动物健康、饲料和食品 安全和安保。

Project Summary/Abstract The New York State/Cornell Animal Health Diagnostic Center (AHDC) is a full-service multidisciplinary veterinary diagnostic laboratory and the only one of its kind in the northeastern United States. The AHDC received over 200,000 client accessions in 2016. The Bacteriology section has MALDI-TOF, Sensititer, Blood culture machine, BSL3 upgradable workspace and 16S DNA sequencing capabilities, making it a full-service bacteriology laboratory. Additionally, the AHDC has in-house ISO approved Whole genome sequencing capacity which are unique to infectious disease laboratories. The Bacteriology laboratory of the AHDC performed approximately 100,000 tests in the year 2016 on clinical samples, tissue, serum, bacterial isolates, environmental, food, and feed samples. The AHDC has partnered with the FDA and played an active role in pet food and animal feed contamination investigations many times over the past dozen years. We hope to continue our participation with the FDA Vet-LIRN program and other network laboratories to: 1) Continue participation in VPO designated sample analyses and surveillance activities to promote animal health and welfare and add to the Vet-LIRN Network's surge capacity to assist in emergency and large-scale outbreak testing. 2) Provide analytical data to support regulatory actions by developing and using standardized methods, equipment platforms, and reporting methods, continued participation in proficiency testing provided by the VPO, continue investigating consumer reported cases as requested by the VPO, and continuing to improve and implement standardized quality management systems as designated by the VPO. 3) Continue to participate in small-scale antibiotic susceptibility testing studies to address emerging antimicrobial resistance issues, and participate in Whole genome sequencing project. The bacteriology section of the AHDC intends to continue cooperative activities with the FDA Vet-LIRN, the Vet- LIRN network, and federal, state, local and tribal organizations to promote animal health and feed and food safety and security.