UltraVIEW VoX spinning disk confocal for core facility

描述（由申请人提供）：这是一份购买PerkinElmer UltraVIEW VoX旋转盘共聚焦显微镜的资金申请，以便为康奈尔大学伊萨卡校区的研究人员提供高速共聚焦成像和光漂白/光激活。该仪器将由显微镜和成像设施（MIF）管理，该设施拥有各种光学成像站，从宽视场显微镜到点扫描共聚焦到全小鼠发光成像仪。现有的点扫描共聚焦镜被高度使用，但MIF设施，像全国各地的许多设施一样，正在看到对活细胞和组织成像的需求增加。新仪器将使测量过程在毫秒时间尺度上的成像速度更快，这是一个只能通过一维点扫描仪访问的制度。对于同等的图像质量，旋转盘共聚焦显微镜在时间推移研究中也显示出明显更少的光毒性，这（显然）对于研究人员在显微镜舞台上成像生物事件的能力至关重要。另一个目前尚未满足的需求是快速光漂白的能力，用于检测动态过程，如膜扩散、微管生长动力学和转录因子结合。拟议中的新型共聚焦显微镜将被安置在威尔大厅，这是一座耗资1.6亿美元的新研究大楼，致力于促进物理科学和生命科学之间的联系。这座新建筑中的研究社区是高度跨学科和快速发展的；预计未来几年将招聘10名新教员；这些员工中的大多数将使用

DESCRIPTION (provided by applicant): This is a request for funds to purchase a PerkinElmer UltraVIEW VoX spinning disk confocal microscope in order to provide high-speed confocal imaging and photobleaching/photoactivation to researchers on Cornell's Ithaca campus. The instrument will be managed under the Microscopy and Imaging Facility (MIF), which houses a variety of optical imaging stations, from widefield microscopes to point scanning confocals to whole mouse luminescence imagers. The existing point scanning confocals are highly used, but the MIF facility, like many across the country, are seeing an increased demand for live cell and tissue imaging. The new instrument will enable faster imaging for measuring processes on the msec timescale, a regime which can only be accessed by point scanners in one dimension. For an equivalent image quality, the spinning disk confocal microscopes have also been shown to be significantly less phototoxic in time lapse studies, which (clearly) can be critical in a researcher's ability to image biological events on the microscope stage. Another currently unmet need has been the capability for fast photobleaching for examining dynamical processes such as membrane diffusion, microtubule growth kinetics and transcription factor binding. The proposed new confocal microscope will be housed in Weill hall, a new $160-million research building dedicated to fostering connections between the physical and life sciences. The research community in this new building is highly interdisciplinary and rapidly growing; 10 new faculty hires are expected in the next several years; the majority of these hires will be using the already overburdened MIF resources. This instrument will directly benefit 6 major and at least 3 minor users, all of whom are PI's of NIH-supported research grants. More broadly it would provide new resources for live cell and tissue imaging on campus, enabling researchers to better understand the fundamental molecular mechanisms of cell signaling, vesicle recycling and secretion, cell-matrix interactions, microtubule dynamics, transcriptional dynamics and establishment of polarity in development. PUBLIC HEALTH RELEVANCE: Human diseases are often the result of molecular associations or signaling events that have gone wrong. The instrument requested will allow researchers to investigate these types of molecular mechanisms in live cells on the microscope stage. The major users of this instrument carry out fundamental biological research with implications to a broad spectrum of human health: cancer, immunology and reproduction and development.