UltraVIEW VoX spinning disk confocal for core facility

描述(由申请人提供)：申请资金购买PerkinElmer UltraView Vox旋转圆盘共焦显微镜，以便为康奈尔大学伊萨卡校区的研究人员提供高速共焦成像和光漂白/光激活。该仪器将在显微镜和成像设施(MIF)的管理下进行管理，该设施拥有各种光学成像站，从广域显微镜到点扫描共焦再到全鼠发光成像仪。现有的点扫描共焦设备使用率很高，但与全国各地的许多设备一样，MIF设备对活细胞和组织成像的需求正在增加。新仪器将使在毫秒时间尺度上测量过程的成像速度更快，这一制度只能由一维的点扫描仪访问。对于同等的图像质量，旋转圆盘共聚焦显微镜在时间推移研究中也被证明光毒性显著降低，这(显然)可能是研究人员在显微镜舞台上成像生物事件的能力的关键。另一个目前尚未满足的需求是快速光漂白的能力，用于检测动态过程，如膜扩散、微管生长动力学和转录因子结合。拟议中的新共焦显微镜将安装在威尔霍尔大厅，这是一座耗资1.6亿美元的新研究大楼，致力于促进物理科学和生命科学之间的联系。这座新大楼的研究社区是高度跨学科和快速增长的；预计在未来几年内将招聘10名新教师；这些招聘人员中的大多数将使用 MIF资源已经不堪重负。该仪器将直接惠及6个主要用户和至少3个次要用户，他们都是美国国立卫生研究院资助的研究基金的PI。更广泛地说，它将为校园内的活细胞和组织成像提供新的资源，使研究人员能够更好地理解细胞信号、囊泡回收和分泌、细胞与基质相互作用、微管动力学、转录动力学和发育中极性建立的基本分子机制。 公共卫生相关性：人类疾病往往是分子关联或信号事件出错的结果。所要求的仪器将允许研究人员在显微镜舞台上研究活细胞中的这些类型的分子机制。该仪器的主要使用者进行基础生物学研究，涉及范围广泛的人类健康：癌症、免疫学以及生殖和发育。

DESCRIPTION (provided by applicant): This is a request for funds to purchase a PerkinElmer UltraVIEW VoX spinning disk confocal microscope in order to provide high-speed confocal imaging and photobleaching/photoactivation to researchers on Cornell's Ithaca campus. The instrument will be managed under the Microscopy and Imaging Facility (MIF), which houses a variety of optical imaging stations, from widefield microscopes to point scanning confocals to whole mouse luminescence imagers. The existing point scanning confocals are highly used, but the MIF facility, like many across the country, are seeing an increased demand for live cell and tissue imaging. The new instrument will enable faster imaging for measuring processes on the msec timescale, a regime which can only be accessed by point scanners in one dimension. For an equivalent image quality, the spinning disk confocal microscopes have also been shown to be significantly less phototoxic in time lapse studies, which (clearly) can be critical in a researcher's ability to image biological events on the microscope stage. Another currently unmet need has been the capability for fast photobleaching for examining dynamical processes such as membrane diffusion, microtubule growth kinetics and transcription factor binding. The proposed new confocal microscope will be housed in Weill hall, a new $160-million research building dedicated to fostering connections between the physical and life sciences. The research community in this new building is highly interdisciplinary and rapidly growing; 10 new faculty hires are expected in the next several years; the majority of these hires will be using the already overburdened MIF resources. This instrument will directly benefit 6 major and at least 3 minor users, all of whom are PI's of NIH-supported research grants. More broadly it would provide new resources for live cell and tissue imaging on campus, enabling researchers to better understand the fundamental molecular mechanisms of cell signaling, vesicle recycling and secretion, cell-matrix interactions, microtubule dynamics, transcriptional dynamics and establishment of polarity in development. PUBLIC HEALTH RELEVANCE: Human diseases are often the result of molecular associations or signaling events that have gone wrong. The instrument requested will allow researchers to investigate these types of molecular mechanisms in live cells on the microscope stage. The major users of this instrument carry out fundamental biological research with implications to a broad spectrum of human health: cancer, immunology and reproduction and development.