Zeiss LSM710 Confocal Microscope for Shared Imaging Facility

用于共享成像设施的蔡司 LSM710 共焦显微镜

• 批准号: 7594974
• 负责人: REBECCA M WILLIAMS
• 金额: $ 49.97万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-14 至 2010-05-13
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7594974
• 关键词: 5 year old; Brain; Cancer Biology; Cartilage; Cell Communication; Cell secretion; Cells; Communities; Country; Development; Diabetes Mellitus; Engineering; Event; Faculty; Funding; Health; Hour; Human; Image; Life; Mediating; Microscope; Microscopy; Molecular; Photobleaching; Principal Investigator; Recycling; Reproduction; Reproductive Process; Research; Research Personnel; Research Project Grants; Resources; Schedule; Signal Transduction; Staging; System; Time; Tissues; Vesicle; human disease; instrument; public health relevance

DESCRIPTION (provided by applicant): This is a request for funds to purchase an inverted Zeiss LSM710 confocal microscope to augment a 5-year old upright Leica TCS SP2 confocal microscope that will remain in the same shared imaging facility. These instruments will be managed under MIF-Biotech, one of two Microscopy and Imaging Facilities (MIF), whose main function is to serve central campus confocal imaging needs. The existing Leica SP2 still functions adequately for many users, but another instrument is necessary. The MIF facility, like many across the country, are seeing an increased demand for live cell and tissue imaging, and currently the system is so overbooked that MIF-Biotech staff needed to implement complicated scheduling rules limiting usage to three hour blocks of time, which are inadequate for researchers wanting to carry out time lapse imaging and photobleaching studies. The new instrument will also enable new capabilities for these studies, such as better photoconversion capabilities and an environmental stage for better maintaining tissues on the stage. MIF-Biotech and the proposed new confocal will move into Weill hall, a $160-million research building now nearing completion. The research community in this new building is highly interdisciplinary and rapidly growing; 10-20 new faculty hires are expected in the next several years, the majority of whom will be using the already overburdened MIF-Biotech resources. This instrument will directly benefit 11 central-campus-residing major users, all of whom are principal investigators of NIH-supported research grants. More broadly it would provide new resources for live cell and tissue imaging on campus, enabling researchers to better understand the fundamental molecular mechanisms of cell signaling, vesicle recycling and secretion, cell-matrix interactions and cell-mediated developmental and reproductive processes. PUBLIC HEALTH RELEVANCE: Human diseases are often the result of molecular associations or signaling events that have gone wrong. The instrument requested will allow researchers to investigate these types of molecular mechanisms in live cells on the microscope stage. The major users of this instrument carry out research with implications to a broad spectrum of human health: reproduction and development, cancer biology, cartilage engineering, diabetes and brain function.

描述（由申请人提供）：这是一份资金申请，用于购买一台倒置蔡司LSM 710共聚焦显微镜，以增强一台使用5年的直立徕卡TCS SP2共聚焦显微镜，该显微镜将保留在同一个共享成像设施中。这些仪器将由MIF-Biotech管理，MIF-Biotech是两个显微镜和成像设施（MIF）之一，其主要功能是满足中央校园共聚焦成像的需求。现有的Leica SP2对许多用户来说仍然可以正常工作，但需要另一台仪器。MIF设施，像全国许多地方一样，对活细胞和组织成像的需求不断增加，目前该系统已经超额预订，MIF-Biotech的工作人员需要实施复杂的调度规则，将使用时间限制在三个小时内，这对于想要进行延时成像和光漂白研究的研究人员来说是不够的。新仪器还将为这些研究提供新的能力，例如更好的光转换能力和更好地保持组织在舞台上的环境舞台。MIF-Biotech和拟议中的新共聚焦将搬进威尔大厅，这座耗资1.6亿美元的研究大楼目前已接近完工。在这座新大楼的研究社区是高度跨学科和快速增长; 10-20个新的教师招聘预计在未来几年内，其中大部分将使用已经不堪重负的MIF-Biotech资源。该工具将直接受益于11个中央校园居住的主要用户，他们都是NIH支持的研究赠款的主要研究者。更广泛地说，它将为校园内的活细胞和组织成像提供新的资源，使研究人员能够更好地了解细胞信号传导、囊泡循环和分泌、细胞-基质相互作用以及细胞介导的发育和生殖过程的基本分子机制。公共卫生相关性：人类疾病通常是分子关联或信号事件出错的结果。所要求的仪器将允许研究人员在显微镜台上研究活细胞中这些类型的分子机制。该仪器的主要用户进行的研究涉及广泛的人类健康：生殖和发育，癌症生物学，软骨工程，糖尿病和脑功能。

项目成果

Ube2V2 Is a Rosetta Stone Bridging Redox and Ubiquitin Codes, Coordinating DNA Damage Responses.

• DOI: 10.1021/acscentsci.7b00556
• 发表时间: 2018-02-28
• 期刊: ACS central science
• 影响因子: 18.2
• 作者: Zhao Y;Long MJC;Wang Y;Zhang S;Aye Y
• 通讯作者: Aye Y

Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants.

• DOI: 10.3389/fgeed.2021.617553
• 发表时间: 2021
• 期刊: Frontiers in genome editing
• 作者: Wang L;Kaya HB;Zhang N;Rai R;Willmann MR;Carpenter SCD;Read AC;Martin F;Fei Z;Leach JE;Martin GB;Bogdanove AJ
• 通讯作者: Bogdanove AJ

SIRT2 and lysine fatty acylation regulate the transforming activity of K-Ras4a.

• DOI: 10.7554/elife.32436
• 发表时间: 2017-12-14
• 期刊: eLife
• 影响因子: 7.7
• 作者: Jing H;Zhang X;Wisner SA;Chen X;Spiegelman NA;Linder ME;Lin H
• 通讯作者: Lin H

Breast cancer-derived extracellular vesicles stimulate myofibroblast differentiation and pro-angiogenic behavior of adipose stem cells.

• DOI: 10.1016/j.matbio.2016.11.008
• 发表时间: 2017-07
• 期刊: Matrix biology : journal of the International Society for Matrix Biology
• 作者: Song YH;Warncke C;Choi SJ;Choi S;Chiou AE;Ling L;Liu HY;Daniel S;Antonyak MA;Cerione RA;Fischbach C
• 通讯作者: Fischbach C

Breast cancer cells alter the dynamics of stromal fibronectin-collagen interactions.

• DOI: 10.1016/j.matbio.2016.08.001
• 发表时间: 2017-07
• 期刊: Matrix biology : journal of the International Society for Matrix Biology
• 作者: Wang K;Wu F;Seo BR;Fischbach C;Chen W;Hsu L;Gourdon D
• 通讯作者: Gourdon D