FACSymphony S6 Cell Sorter for Improving Basic, Clinical, and Translational Cancer Research Capabilities

项目摘要/摘要 流式细胞仪共享资源(FCSR)是一项全面的核心设施，已成为 自1990年以来一直是加州大学圣地亚哥分校摩尔癌症中心(MCC)的组成部分。 它为所有MCC研究计划和La Jolla生物医学研究社区提供随时可用的访问 临床分离细胞群的高速流式细胞术分析和细胞分选 样本、动物实验和细胞培养研究。FCSR一直是一个利用率很高且必不可少的核心 对MCC的各种研究项目至关重要的设施。2013-2018年间，FCSR为59家MCC提供服务 并被MCC调查人员利用了近4000小时。然而，流式细胞仪的最新进展 技术已经使FCSR中的现有分拣机大多过时，无法满足最新技术的要求 ART研究需要，这有可能限制癌症研究的关键进展。位于中的两个单元格分选器 FCSR是BD FACSAria I和FACSAria II，分别超过15岁和13岁。BD不再支持 FACSAria I的服务合同，因此，该文书大部分已交付 无能为力。虽然FACSAria II在某种程度上仍在运行，但它的年龄和配置(4台激光，13色，4- 方法分类)不足以满足当前的研究需要。此外，FACSAria II不是安装在 生物安全柜，限制其用于对某些生物危险样本进行分类，包括一些人类临床样本 样本。随着MCC高级领导层认识到这些缺点，振兴MCC的全面计划 研制了FCSR。为此，他们任命Siger博士为#年FCSR的新任联席主任 负责新的仪器设备。需要资金来购买BD FACSYMPHONS6高参数单元 分类器，一个台式高速细胞分类器，配备了五个激光(355,405,488,561和637 nm)，能够 分析多达23种不同的颜色加上正向和侧向散射。FAC交响乐团将包含在 Baker Class II A2型生物安全柜可满足临床样本分类的安全和法规要求。 这一文书是推动MCC高级领导人实现现代化愿景的关键的早期步骤 MCC FCSR。结合专业知识、领导力和培训，BD FACSymphony S6细胞分选器 Siger博士和工作人员的计划将确保加州大学圣地亚哥分校和周围科学研究所的研究人员 社区再次可以一天24小时、一周7天、一年365天使用细胞分选技术 他们需要开展高影响力的癌症研究。如果没有这个工具，FCSR将继续拥有 仪器设备严重受限，MCC教职员工的研究将受到损害。收购屋宇署 FACSymphony S6细胞分选器将使FCSR能够继续其核心使命，提供最先进的 为MCC调查人员和La Jolla生物医学提供仪器、专家支持、教育和培训 以高效、可靠和具有成本效益的方式在研究界开展工作。

PROJECT SUMMARY/ABSTRACT The Flow Cytometry Shared Resource (FCSR) is a comprehensive core facility that has been an integral component of the Moores Cancer Center (MCC) at the University of California, San Diego (UCSD) since 1990. It serves all MCC research programs and the La Jolla biomedical research community at large with ready access to high-speed flow cytometry-based analysis and cell sorting of dissociated cell populations from clinical samples, animal experiments, and cell culture studies. The FCSR has been a highly utilized and essential core facility critical to diverse research programs in the MCC. Between 2013-2018, the FCSR served 59 MCC investigators and was utilized for nearly 4000 hours by MCC investigators. However, recent advances in FACS technology have rendered current sorters in the FCSR mostly obsolete and incapable of meeting state-of-the- art research needs, which threatens to limit critical advances in cancer research. The two cell sorters located in the FCSR, a BD FACSAria I and FACSAria II, are over 15 and 13 years old, respectively. BD no longer supports service contracts for the FACSAria I, and as a consequence, the instrument has been rendered mostly inoperative. While the FACSAria II is still somewhat operational, its age and configuration (4 laser, 13 color, 4- way sorting) are insufficient to meet current research needs. In addition, the FACSAria II is not housed within a biosafety cabinet, which limits its use for sorting certain biohazardous samples, including some human clinical samples. With these shortcomings recognized by senior MCC leadership, a comprehensive plan to revitalize the FCSR was developed. Towards that end, they have appointed Dr. Signer as the new co-Director of the FCSR in charge of new instrumentation. Funds are requested to acquire the BD FACSymphony S6 high parameter cell sorter, a bench-top high speed cell sorter equipped with five lasers (355, 405, 488, 561, and 637 nm) capable of analyzing up to 23 different colors plus forward and side scatter. The FACSymphony will be contained within a Baker Class II Type A2 biosafety cabinet to meet safety and regulatory requirements for sorting clinical samples. This instrument represents a crucial early step in advancing the MCC senior leaders’ vision to modernize the MCC FCSR. The BD FACSymphony S6 cell sorter when combined with the expertise, leadership, and training plans of Dr. Signer and staff will ensure that researchers at UCSD and within the surrounding scientific community once again have access 24 hours a day, 7 days a week, 365 days a year to the cell sorting technology they need to carry out high-impact cancer research. Without this instrument, the FCSR will continue to have severely limited instrumentation, and MCC faculty research will be impaired. Acquisition of the BD FACSymphony S6 cell sorter will enable the FCSR to continue its central mission to provide state-of-the-art instrumentation, expert support, education, and training for MCC investigators and the La Jolla biomedical research community in a highly effective, reliable and cost-efficient manner.