FACSymphony S6 Cell Sorter for Improving Basic, Clinical, and Translational Cancer Research Capabilities

项目总结/摘要 流式细胞仪共享资源（FCSR）是一个综合性的核心设施， 自1990年以来，在加州大学圣地亚哥分校（UCSD）的摩尔癌症中心（MCC）的组成部分。 它服务于所有MCC研究计划和拉霍亚生物医学研究社区， 涉及对来自临床的分离的细胞群的基于高速流式细胞术的分析和细胞分选 样品、动物实验和细胞培养研究。《财务和社会责任报告》一直是一个利用率很高的重要核心 该设施对MCC的各种研究计划至关重要。2013 - 2018年间，FCSR为59 MCC提供服务 调查员使用了近4 000个小时。然而，流式细胞仪的最新进展 技术已经使得FCSR中的当前分拣机大多过时，不能满足当前的需求。 这可能会限制癌症研究的重大进展。两个细胞分选仪位于 FCSR，即BD FACSAria I和FACSAria II，分别超过15年和13年。BD不再支持 因此，该仪器已交付的大部分是 - 是的虽然FACSAria II仍在一定程度上运行，但其年龄和配置（4激光，13色，4- 方法排序）不足以满足当前的研究需求。此外，FACSAria II不在 生物安全柜，这限制了其用于分选某些生物危害性样本，包括一些人类临床 样品由于管理协委会高级领导层认识到这些缺点， 开发了FCSR。为此，他们于2004年任命Signer博士为财务和社会研究中心的新共同主任。 新仪器的费用。申请资金用于购买BD FACSymphony S6高参数电池 分选机，配备有五个激光器（355、405、488、561和637 nm）的台式高速细胞分选机，能够 分析多达23种不同的颜色以及前向和侧向散射。FACSymphony将包含在一个 Baker II类A2型生物安全柜符合临床样本分类的安全和法规要求。 这一工具是促进千年挑战委员会高级领导人实现千年挑战委员会现代化愿景的重要早期步骤。 MCC FCSR。BD FACSymphony S6细胞分选仪结合专业知识、领导力和培训 Signer博士和工作人员的计划将确保UCSD的研究人员和周围的科学 社区再次有机会获得每天24小时，每周7天，一年365天的细胞分选技术 他们需要进行高影响力的癌症研究。如果没有这一文书， 严重有限的仪器，和MCC教师的研究将受到损害。收购BD FACSymphony S6细胞分选机将使FCSR能够继续其核心使命，提供最先进的 仪器，专家支持，教育和培训MCC研究人员和拉霍亚生物医学 以高效、可靠和具有成本效益的方式为研究界提供服务。

PROJECT SUMMARY/ABSTRACT The Flow Cytometry Shared Resource (FCSR) is a comprehensive core facility that has been an integral component of the Moores Cancer Center (MCC) at the University of California, San Diego (UCSD) since 1990. It serves all MCC research programs and the La Jolla biomedical research community at large with ready access to high-speed flow cytometry-based analysis and cell sorting of dissociated cell populations from clinical samples, animal experiments, and cell culture studies. The FCSR has been a highly utilized and essential core facility critical to diverse research programs in the MCC. Between 2013-2018, the FCSR served 59 MCC investigators and was utilized for nearly 4000 hours by MCC investigators. However, recent advances in FACS technology have rendered current sorters in the FCSR mostly obsolete and incapable of meeting state-of-the- art research needs, which threatens to limit critical advances in cancer research. The two cell sorters located in the FCSR, a BD FACSAria I and FACSAria II, are over 15 and 13 years old, respectively. BD no longer supports service contracts for the FACSAria I, and as a consequence, the instrument has been rendered mostly inoperative. While the FACSAria II is still somewhat operational, its age and configuration (4 laser, 13 color, 4- way sorting) are insufficient to meet current research needs. In addition, the FACSAria II is not housed within a biosafety cabinet, which limits its use for sorting certain biohazardous samples, including some human clinical samples. With these shortcomings recognized by senior MCC leadership, a comprehensive plan to revitalize the FCSR was developed. Towards that end, they have appointed Dr. Signer as the new co-Director of the FCSR in charge of new instrumentation. Funds are requested to acquire the BD FACSymphony S6 high parameter cell sorter, a bench-top high speed cell sorter equipped with five lasers (355, 405, 488, 561, and 637 nm) capable of analyzing up to 23 different colors plus forward and side scatter. The FACSymphony will be contained within a Baker Class II Type A2 biosafety cabinet to meet safety and regulatory requirements for sorting clinical samples. This instrument represents a crucial early step in advancing the MCC senior leaders’ vision to modernize the MCC FCSR. The BD FACSymphony S6 cell sorter when combined with the expertise, leadership, and training plans of Dr. Signer and staff will ensure that researchers at UCSD and within the surrounding scientific community once again have access 24 hours a day, 7 days a week, 365 days a year to the cell sorting technology they need to carry out high-impact cancer research. Without this instrument, the FCSR will continue to have severely limited instrumentation, and MCC faculty research will be impaired. Acquisition of the BD FACSymphony S6 cell sorter will enable the FCSR to continue its central mission to provide state-of-the-art instrumentation, expert support, education, and training for MCC investigators and the La Jolla biomedical research community in a highly effective, reliable and cost-efficient manner.