Mechanistic characterization of SARS-CoV2 associated kidney injury
SARS-CoV2相关肾损伤的机制特征
基本信息
- 批准号:10427448
- 负责人:
- 金额:$ 39.38万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-06-11 至 2024-04-30
- 项目状态:已结题
- 来源:
- 关键词:2019-nCoVAcuteAcute Renal Failure with Renal Papillary NecrosisAcute Respiratory Distress SyndromeAgeApoptosisCOVID-19COVID-19 complicationsCOVID-19 patientCRISPR screenCaspaseCell DeathCellsCessation of lifeClinical ResearchClustered Regularly Interspaced Short Palindromic RepeatsCoculture TechniquesCohort AnalysisComplexCoronavirusDataDiseaseEtiologyFunctional disorderGenesGoalsHMGB1 geneHistopathologyHumanISG15 geneIn VitroIndividualInfectionInflammationInflammatoryInflammatory ResponseInjuryInjury to KidneyIntegration Host FactorsInterferonsK-18 conjugateKidneyKnockout MiceLungMapsMediatingModelingMolecularMusOrganOrganoidsPathogenesisPathway interactionsPatientsPatternPersonsPlayPredispositionProductionProtein KinaseProteinsPulmonary InflammationRIPK1 geneRegulationReperfusion InjuryReportingRisk FactorsRoleSARS coronavirusSARS-CoV-2 infectionSARS-CoV-2 pathogenesisSerumSeverity of illnessSourceSystemTestingTherapeuticTracheal EpitheliumTransgenic MiceTropismTubular formationUbiquitin Like ProteinsUnited StatesViralViral Load resultViral ProteinsVirusVirus DiseasesWorkbasecell injurychemokinecytokinecytokine release syndromeeffective interventiongenome-widehuman coronavirusin vivoinduced pluripotent stem cellinsightkidney cellkidney epithelial cellmortality riskmouse modelnovelrenal damageresponsesevere COVID-19systemic inflammatory responsetherapeutic development
项目摘要
SARS-CoV2 is a highly contagious, novel human coronavirus that causes coronavirus disease 2019 (COVID-
19). Currently over 16.5 million people in the US have confirmed infection with SARS-CoV2 and over 300,000
have died. Severe COVID-19 is characterized by pulmonary and systemic inflammation and multi-organ
dysfunction, with a significant portion of severe COVID patients developing acute kidney injury. The mechanism
by which SARS-CoV2 triggers such severe pathogenesis is poorly understood. Recent clinical studies have
suggested that cell death, especially the induction of necroptosis, may be a predictor of severe COVID-19
disease. The mechanism by which the host restricts necroptosis is unclear. In preliminary data we have shown
that the interferon induced protein, ISG15, acts as a negative regulator of necroptosis and its downstream
inflammatory responses during viral infection. We have also shown that ISG15 deficient mice rapidly succumb
to ischemia-reperfusion injury of the kidney characterized by a massive release of proinflammatory cytokines.
In this proposal we will test the hypothesis that ISG15 serves as a critical host restriction factor in regulating
programmed necroptosis and downstream inflammatory responses within the kidney to limit acute kidney injury
during SARS-CoV2 infection. We will utilize kidney organoids derived from induce pluripotent stem cells in which
ISG15 has been deleted by CRISPR, a co-culture system with kidney organoids and primary tracheal epithelial
cultures (hTECs), and in vivo mouse model of SARS-CoV-2 to ask several questions including: 1) Does SARS-
CoV2 induce damage to kidney epithelial cells via direct viral transduction or in response to systemic
inflammation?; 2) Does ISG15 modulate necroptotic cell death as well as proinflammatory cytokine/chemokine
production in kidney epithelial cells during SARS-CoV2 infection?; 3) Does necroptosis and its regulation by
ISG15 contribute to acute kidney injury during SARS-CoV2 infection? Overall, our studies will provide important
insight into host factors that restrict necroptosis and could be an important contributor to severe COVID-19
induced kidney injury.
SARS-CoV2是一种高度传染性的新型人类冠状病毒,可导致2019年冠状病毒病(COVID-
19)。目前,美国已有超过1650万人确认感染了SARS-CoV2病毒,其中超过30万人感染了SARS-CoV2
都死了。重症新冠肺炎以肺部和全身炎症、多器官损害为特征
严重的COVID患者中有很大一部分会出现急性肾损伤。这一机制
SARS-CoV2通过什么机制触发如此严重的发病机制尚不清楚。最近的临床研究表明
提示细胞死亡,尤其是坏死性下垂的诱导,可能是严重新冠肺炎的一个预测因子
疾病。宿主限制坏死性下垂的机制尚不清楚。在初步数据中,我们已经显示
干扰素诱导蛋白ISG15对坏死性下垂及其下游起负调节作用
病毒感染时的炎症反应。我们还发现ISG15基因缺陷的小鼠会迅速死亡
以促炎症细胞因子大量释放为特征的肾脏缺血再灌注损伤。
在这项建议中,我们将检验ISG15作为关键的宿主限制因子在调节
程序性坏死性下垂和肾脏下游炎症反应限制急性肾损伤
在SARS-CoV2感染期间。我们将利用从诱导多能干细胞中提取的肾脏器官类物质
ISG15已被CRISPR删除,CRISPR是一种与肾脏器官和原代气管上皮共同培养的系统
培养(HTECs),并在体内建立SARS-CoV-2小鼠模型,提出几个问题,包括:1)SARS-
CoV2通过直接病毒转导或全身性免疫应答诱导肾上皮细胞损伤
炎症?;2)ISG15是否与促炎细胞因子/趋化因子一样调节坏死性细胞死亡
SARS-CoV2感染过程中肾上皮细胞产生?;3)坏死性下垂及其调节
ISG15在SARS-CoV2感染中导致急性肾损伤吗?总体而言,我们的研究将提供重要的
洞察限制坏死性下垂的宿主因素可能是严重新冠肺炎的重要因素
致肾损伤。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Deborah J Lenschow其他文献
Deborah J Lenschow的其他文献
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{{ truncateString('Deborah J Lenschow', 18)}}的其他基金
Mechanistic characterization of SARS-CoV2 associated kidney injury
SARS-CoV2相关肾损伤的机制特征
- 批准号:
10319713 - 财政年份:2021
- 资助金额:
$ 39.38万 - 项目类别:
Mechanistic characterization of SARS-CoV2 associated kidney injury
SARS-CoV2相关肾损伤的机制特征
- 批准号:
10619568 - 财政年份:2021
- 资助金额:
$ 39.38万 - 项目类别:
Regulation of Cell Death and Inflammation by ISG15 during SARS-CoV2 Infection
SARS-CoV2 感染期间 ISG15 对细胞死亡和炎症的调节
- 批准号:
10287787 - 财政年份:2021
- 资助金额:
$ 39.38万 - 项目类别:
Regulation of Cell Death and Inflammation by ISG15 during SARS-CoV2 Infection
SARS-CoV2 感染期间 ISG15 对细胞死亡和炎症的调节
- 批准号:
10424558 - 财政年份:2021
- 资助金额:
$ 39.38万 - 项目类别:
Washington University Rheumatic DiseasesResearch Resource-based Center
华盛顿大学风湿病研究资源中心
- 批准号:
10472003 - 财政年份:2018
- 资助金额:
$ 39.38万 - 项目类别:
Washington University Rheumatic DiseasesResearch Resource-based Center
华盛顿大学风湿病研究资源中心
- 批准号:
9764270 - 财政年份:2018
- 资助金额:
$ 39.38万 - 项目类别:
Washington University Rheumatic DiseasesResearch Resource-based Center
华盛顿大学风湿病研究资源中心
- 批准号:
10019327 - 财政年份:2018
- 资助金额:
$ 39.38万 - 项目类别:
Washington University Rheumatic DiseasesResearch Resource-based Center
华盛顿大学风湿病研究资源中心
- 批准号:
10251236 - 财政年份:2018
- 资助金额:
$ 39.38万 - 项目类别:
Washington University Rheumatic Diseases Research Resource-based Center
华盛顿大学风湿病研究资源中心
- 批准号:
10704273 - 财政年份:2018
- 资助金额:
$ 39.38万 - 项目类别:
REGULATION OF INFLUENZA VIRUS INFECTION BY ISG15
ISG15 对流感病毒感染的监管
- 批准号:
8109260 - 财政年份:2009
- 资助金额:
$ 39.38万 - 项目类别:
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