Washington University Rheumatic Diseases Research Resource-based Center

华盛顿大学风湿病研究资源中心

• 批准号: 10704273
• 负责人: Deborah J Lenschow
• 金额: $ 77.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10704273
• 关键词: Acceleration; Address; Adopted; Area; Autoimmunity; Basic Science; Biomedical Engineering; COVID-19 pandemic; Cell Line; Cell Therapy; Clinical; Clinical Data; Clinical Research; Collaborations; Collection; Complex; Core Facility; Cytometry; Data; Data Collection; Deposition; Discipline; Disease; Disease model; Education; Electronic Health Record; Environment; Evidence based program; Extramural Activities; Fostering; Funding; Genome engineering; Goals; Grant; Health; Image; Inflammation; Infrastructure; Knock-in; Knockout Mice; Laboratories; Lupus; Mentors; Microscopy; Modeling; Molecular Analysis; Pathogenesis; Pathway interactions; Patients; Phenotype; Philosophy; Physicians; Positioning Attribute; Postbaccalaureate; Regenerative Medicine; Reporting; Research; Research Personnel; Resources; Rheumatism; Rheumatology; Scientist; Services; Talents; Technology; Testing; Training; Translating; Translational Research; Universities; Washington; biobank; cellular imaging; cost effective; design; health care disparity; high dimensionality; improved; innovative technologies; interest; lupus registry; member; model building; multidisciplinary; multimodality; new technology; next generation; novel; novel therapeutic intervention; pre-clinical; precision medicine; programs; recruit; repository; response; social; social health determinants; transcriptomics

OVERALL ABSTRACT The WU-RDRRC seeks to advance the health of patients with rheumatic diseases by supporting enabling technology and promoting the members’ basic, clinical, and translational research interests that are organized around three major themes: 1) elucidating basic mechanisms of inflammation and autoimmunity; 2) accelerating clinical/translational research to inform precision medicine; and 3) advancing genome engineering and regenerative medicine to develop new treatment approaches for rheumatic diseases. We posit that translational research endeavors in rheumatic diseases require a team approach cultivated in a vibrant environment and supported by cross-disciplinary groups of experts and cutting-edge technologies. Over the past four years, this operational philosophy and the infrastructure supported by the WU-RDRRC perfectly positioned our investigators to increase the efficiency and impact of their research, allowed them to quickly address emergent issues that arose during the COVID-19 pandemic, and helped a number of young investigators obtain extramural funding and successfully transition to research independence. The WU- RDRRC propose to continue promoting rheumatic disease research by: 1) providing the infrastructure, education, and training needed to assist investigators with the experimental and scientific design of their projects; 2) organizing and supporting Core laboratories that will adopt and apply innovative technologies and expertise that would otherwise be unavailable to investigators in a cost-effective manner; 3) promoting interest in rheumatic diseases research by engaging multidisciplinary teams and bringing together diverse disciplines and clinical divisions to address roadblocks in translational science; 4) fostering the next generation of junior investigators who are interested in the study of rheumatic disease-related areas. To accomplish our goals we propose four cores: 1) a Biobank and Phenotyping Core to collect high-quality biospecimens for downstream molecular analysis; 2) a Genome Engineering Core to generate bioengineered cell-based therapies, knockin/knockout mice for testing in preclinical disease models; 3) a Cellular Imaging Core that offers novel microscopy technologies for building models of health and disease; and 4) an Administrative Core that promotes collaborative and synergistic interactions among rheumatic disease researchers and the mentoring of junior and new investigators interested in rheumatic disease research. In addition, we will address members’ evolving needs by expanding access to novel technologies such as spatial transcriptomics, and the changing societal landscape by incorporating social determinants of health into clinical data collection, and enhancing diversity of trainees entering the physician-scientist pathway.

总体摘要 WU-RDRRC寻求通过支持使风湿性疾病患者的健康 技术和促进成员的基础，临床和转化的研究兴趣， 围绕三大主题：1）阐明炎症和自身免疫的基本机制; 2） 加速临床/转化研究，为精准医学提供信息; 3）推进基因组工程 和再生医学来开发风湿性疾病的新治疗方法。 我们认为，风湿性疾病的转化研究工作需要一个团队的方法培养， 充满活力的环境，并得到跨学科专家组和尖端技术的支持。 在过去四年中，这一业务理念和由WU-RDRRC支持的基础设施 完美地定位我们的调查人员，以提高他们的研究效率和影响，使他们能够 迅速解决COVID-19大流行期间出现的紧急问题，并帮助一些年轻人 研究人员获得校外资助，并成功地过渡到研究独立。WU- RDRRC建议通过以下方式继续促进风湿病研究：1）提供基础设施， 教育和培训，以帮助研究人员进行实验和科学设计， 2）组织和支持采用和应用创新技术的核心实验室， 以具有成本效益的方式提供调查人员本来无法获得的专门知识; 3）提高兴趣 在风湿性疾病的研究，通过从事多学科团队，并汇集不同学科 和临床部门，以解决转化科学的障碍; 4）培养下一代初级 对风湿性疾病相关领域研究感兴趣的研究人员。 为了实现我们的目标，我们提出了四个核心：1）生物库和表型核心，以收集高质量的 用于下游分子分析的生物标本; 2）基因组工程核心，以产生生物工程 基于细胞的疗法，用于在临床前疾病模型中测试的敲入/敲除小鼠; 3）细胞成像 核心，为建立健康和疾病模型提供新的显微镜技术;以及4） 促进风湿性疾病之间协作和协同作用的管理核心 研究人员以及对风湿性疾病研究感兴趣的初级和新研究人员的指导。 此外，我们将通过扩大对新技术的使用， 空间转录组学，以及通过将健康的社会决定因素纳入 临床数据收集，并加强学员进入医生-科学家途径的多样性。

项目成果

Dysregulated NK cell PLCγ2 signaling and activity in juvenile dermatomyositis.

青少年皮肌炎中 NK 细胞 PLCγ2 信号传导和活性失调。

• DOI: 10.1172/jci.insight.123236
• 发表时间: 2018
• 期刊: JCI insight
• 影响因子: 8
• 作者: Throm,AllisonA;Alinger,JoshuaB;Pingel,JeanetteT;Daugherty,AllyssaL;Pachman,LaurenM;French,AnthonyR
• 通讯作者: French,AnthonyR

Validation of a commercial antibody to detect endogenous human nicastrin by immunoblot.

通过免疫印迹验证商业抗体检测内源性人尼卡斯特林。

• DOI: 10.12688/f1000research.19803.2
• 发表时间: 2019
• 期刊: F1000Research
• 作者: Mesa,RosanaA;Roberson,ElishaDO
• 通讯作者: Roberson,ElishaDO

Clinicomolecular Identification of Conserved and Individualized Features of Granulomatous Uveitis.

肉芽肿性葡萄膜炎保守和个体化特征的临床分子鉴定。

• DOI: 10.1016/j.xops.2021.100010
• 发表时间: 2021
• 期刊: Ophthalmology science
• 作者: Hassman,LynnM;Paley,MichaelA;Esaulova,Ekaterina;Paley,GraceL;Ruzycki,PhilipA;Linskey,Nicole;Laurent,Jennifer;Feigl-Lenzen,Lacey;Springer,Luke;Montana,CynthiaL;Hong,Karen;Enright,Jennifer;James,Hayley;Artyomov,MaximN;Yokoy
• 通讯作者: Yokoy

Reduced SPAG17 Expression in Systemic Sclerosis Triggers Myofibroblast Transition and Drives Fibrosis.

• DOI: 10.1016/j.jid.2022.08.052
• 发表时间: 2023-02
• 期刊: JOURNAL OF INVESTIGATIVE DERMATOLOGY
• 影响因子: 6.5
• 作者: Sapao, Paulene;Roberson, Elisha D. O.;Shi, Bo;Assassi, Shervin;Skaug, Brian;Lee, Fred;Naba, Alexandra;White, Bethany E. Perez;Cerdova-Fletes, Carlos;Tsou, Pei-Suen;Sawalha, Amr H.;Gudjonsson, Johann E.;Ma, Feiyang;Verma, Priyanka;Bhattacharyya, Dibyendu;Carns, Mary;Strauss III, Jerome F.;Sicard, Delphine;Tschumperlin, Daniel J.;Champer, Melissa I.;Campagnola, Paul J.;Teves, Maria E.;Varga, John
• 通讯作者: Varga, John

Emerging roles of the complement system in host-pathogen interactions.

• DOI: 10.1016/j.tim.2021.09.002
• 发表时间: 2022-04
• 期刊: Trends in microbiology
• 影响因子: 15.9
• 作者: Sahu SK;Kulkarni DH;Ozanturk AN;Ma L;Kulkarni HS
• 通讯作者: Kulkarni HS