Washington University Rheumatic DiseasesResearch Resource-based Center

总体摘要 基于资源的华盛顿大学风湿病研究的总体目标 中心(WU-RDRRC)通过提供基础设施、资源和资源来促进风湿病的研究 和机会进行多层次的调查，并将这些发现转化为治疗方法，以便交付给 病人。WU-RDRRC旨在通过支持风湿病患者的健康来促进他们的健康 使技术成为可能，并促进其成员的基础和翻译研究兴趣。这些 兴趣围绕三个主要主题组织：(1)炎症和自身免疫的基本机制； (2)翻译基因组学和生物标志物的发现；(3)肌肉骨骼和再生医学。 我们的中心假设是，风湿病研究中的翻译工作需要一个团队 在充满活力的环境中实施的方法，由跨学科的专家团队和国家 艺术服务。我们的重点是促进互动方法，以加快创新的步伐 发现并将发现转化为风湿病患者治疗的改进。 为了实现我们的目标，我们提出了以下建议：1)翻译研究核心(TRC)，包括 致力于支持翻译研究工作的独立但相关的服务：i)综合 风湿病登记处和生物库(Biobank)，可通过电子方式进行检索 生物医学数据管理系统，允许对组织和数据库进行注释、跟踪和共享；二)a 基因组学和生物信息学设施(GBF)由最新的下一代测序技术提供支持 加强人类基因组数据安全使用和分析的技术和生物信息学工具和三) 一种免疫监测设施(IMF)，提供用于分析人体样本的质量细胞术。2)A 基因组工程核心(GEC)，通过使用技术进步促进功能研究 例如CRISPR/Cas9，以产生突变细胞系和基因敲除/敲入小鼠并诱导多能干细胞 细胞(IPSC)；以及3)细胞成像核心(CIC)，提供最先进的成像技术 建立健康和疾病的模型。行政核心促进协作和协同 风湿病研究中跨多个学科的相互作用并促进对新的 对风湿病相关研究感兴趣的研究人员。

OVERALL ABSTRACT The overarching Goal of the Washington University Rheumatic Diseases Research Resource-based Center (WU-RDRRC) is to advance research in rheumatic diseases by providing the infrastructure, resources and opportunities for multiple levels of inquiry and translating those discoveries into treatments for delivery to patients. The WU-RDRRC seeks to advance the health of patients with rheumatic disease by supporting enabling technology and promoting the basic and translational research interests of its members. These interests are organized around three major themes: (1) Basic mechanisms of inflammation and autoimmunity; (2) Translational genomics and biomarker discovery; and (3) Musculoskeletal and Regenerative Medicine. Our Central Hypothesis is that translational endeavors in rheumatic diseases research require a team approach performed in a vibrant environment supported by a cross-disciplinary team of experts and state-of- the-art services. Our Focus is to promote interactive approaches that will accelerate the pace of innovative discoveries and translate findings to improvements in treatments for patients with rheumatic diseases. To accomplish our goals we propose the following: 1) A Translational Research Core (TRC) that comprises separate but related services devoted to supporting translational research endeavors: i) an integrated rheumatic disease registry and biospecimen repository (Biobank) that can be searched through electronic biomedical data management systems to allow annotation, tracking and sharing of tissue and databases; ii) a Genomics & Bioinformatics Facility (GBF) powered by the most up-to-date Next-Generation Sequencing technologies and bioinformatics tools to enhance the secure use and analysis of human genomic data and iii) an ImmunoMonitoring Facility (IMF) that offers access to mass cytometry for analysis of human samples. 2) A Genome Engineering Core (GEC) that facilitates functional studies through the use of technological advances such as CRISPR/Cas9 to generate mutant cell lines and knockout/knock-in mice and induced Pluripotent Stem Cells (iPSCs); and 3) A Cellular Imaging Core (CIC) that provides state-of-the-art imaging technologies for building models of health and disease. The Administrative Core promotes collaborative and synergistic interactions in rheumatic diseases research across multiple disciplines and fosters the mentoring of new investigators interested in rheumatic disease-related research.