权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

HNF1A in human endocrine cell development and function

HNF1A 在人类内分泌细胞发育和功能中的作用

基本信息

批准号：
10427273
负责人：
PAUL J GADUE
金额：
$ 51.89万
依托单位：
CHILDREN'S HOSP OF PHILADELPHIA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-20 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10427273
关键词：
Affect Age Alpha Cell Animals Autoimmune Diseases Beta Cell Biological Assay Biological Models CRISPR/Cas technology Cell Line Cell physiology Cells Cellular Stress Cellular biology Child Code Complement Complex Cre driver Data Set Defect Development Diabetes Mellitus Diagnosis Disease Down-Regulation Endocrine Gene Expression Profiling Genes Genetic Genome engineering Genomics Glycolysis Goals High Prevalence Human Hypoxia In Vitro Individual Inherited Insulin Insulin-Dependent Diabetes Mellitus Islets of Langerhans Link LoxP-flanked allele Metabolic Metabolic Diseases Mitochondria Modeling Mouse Strains Mus Mutate Mutation Non-Insulin-Dependent Diabetes Mellitus Patients Penetrance Phenotype Pluripotent Stem Cells Proto-Oncogene Proteins c-akt Rare Diseases Regulation Rodent Rodent Model Role Signal Transduction Untranslated RNA Variant Xenograft Model base cohort diabetic diabetic patient differentiation protocol endocrine pancreas development exhaustion experimental study genome wide association study genome-wide human disease human embryonic stem cell line human model human pluripotent stem cell human stem cells in vitro Model in vivo insulin secretion maturity onset diabetes of the young metabolic phenotype mouse genome mouse model mutant novel overexpression pancreas development response stem stem cell model stem cells stressor transcription factor type I and type II diabetes virtual

项目摘要

Diabetes is a complex disorder with three main subtypes, type I, type II and monogenic. Type I is an autoimmune disorder characterized by loss of the insulin secreting beta cell. Type II is metabolic disorder characterized by poor insulin responsiveness and eventual beta cell exhaustion and loss. Monogenic forms are caused by mutations in a number of genes that are generally important in beta cell development and/or function. This proposal will focus on the study on HNF1A, a transcription factor that when mutated leads to the most common cause on monogenic diabetes. In addition, HNF1A has been linked to both type I and type II diabetes in GWAS studies. Mouse models of HNF1A deficiency are available, but unfortunately do not appropriately mimic the human disease. Therefore, use of a human model system is important. We will use the human pluripotent stem cell model to study the role of HNF1A in both endocrine cell development and function, utilizing CRISPR-Cas9 based genome engineering to generate isogenic mutant lines. Robust in vitro differentiation protocols exist and will be used to generate beta cells in the human stem cell model. In preliminary studies, we have uncovered human specific HNF1A targets that have been validated in a dataset from an individual with monogenic diabetes due to a HNF1A mutation. We have also discovered phenotypes in metabolic functions in HNF1A mutant stem cell derived beta cells, offering a potential link between the association of HNF1A with type II and possibly type I diabetes. Lastly, one of the human specific targets of HNF1A is a long non-coding RNA that when deleted in stem cells, mimics some of the metabolic phenotypes seen in the HNF1A mutants. These studies will help define the developmental and functional requirements of HNF1A in pancreatic endocrine cells and have the potential to directly impact the treatment of not only rare monogenic forms of diabetes but also type I and type II diabetes.

糖尿病是一种复杂的疾病，有三个主要亚型，I型、II型和单基因。I型是一种自身免疫性疾病，其特征是失去分泌胰岛素的β细胞。II型是一种代谢紊乱，其特征是胰岛素反应性差，最终导致β细胞衰竭和丧失。单基因形式是由一些基因的突变引起的，这些基因通常对β细胞的发育和/或功能很重要。这项提案将重点研究HNF1A，这是一种转录因子，当突变时会导致单基因糖尿病最常见的原因。此外，在GWAS的研究中，HNF1a与I型和II型糖尿病都有关联。HNF1A缺乏症的小鼠模型是可用的，但不幸的是，它们不能适当地模拟人类疾病。因此，使用人体模型系统很重要。我们将使用人类多能干细胞模型来研究HNF1A在内分泌细胞发育和功能中的作用，利用基于CRISPR-Cas9的基因组工程来产生等基因突变株。强大的体外分化方案已经存在，并将用于在人类干细胞模型中产生贝塔细胞。在初步研究中，我们发现了人类特异的HNF1A靶点，这些靶点已经在一名因HNF1A突变而患有单基因糖尿病的患者的数据集中得到验证。我们还发现了HNF1a突变干细胞来源的β细胞代谢功能的表型，提供了HNF1a与II型糖尿病和可能的I型糖尿病之间的潜在联系。最后，HNF1A的人类特异性靶点之一是一个长的非编码RNA，当在干细胞中被删除时，它模仿了HNF1A突变体中看到的一些代谢表型。这些研究将有助于确定HNF1A在胰腺内分泌细胞中的发育和功能需求，并有可能不仅直接影响罕见的单基因形式糖尿病的治疗，而且还可能影响I型和II型糖尿病的治疗。