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Project 3

项目3

基本信息

批准号：
10428169
负责人：
Frances Eun-Hyung Lee
金额：
$ 51.66万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-06-25 至 2027-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10428169
关键词：
Actins Address Affect Antibodies Apoptosis Autophagocytosis BCL2 gene Blood Bone Marrow CD19 gene Cell Aging Cell Cycle Cell Cycle Arrest Cell Maturation Cell Nucleus Cell Shape Cell Size Cell Survival Cell secretion Cells Cues Cyclin-Dependent Kinase Inhibitor Cytoplasm Disease Endoplasmic Reticulum Epigenetic Process Family Fibroblast Growth Factor Funding GRP78 gene Generations Genetic Genetic Transcription Genus Mentha Health Human Hypoxia IL8 gene Immunoglobulin G Immunoglobulin-Secreting Cells In Vitro Interleukin-1 Interphase Cell Link Maintenance Measures Mediating Mitochondria Molecular Chaperones Morphology NF-kappa B Pathway interactions Phenotype Plasma Cells Play Population Process Proteins Recording of previous events Refractory Regulation Resistance Role Serum Specificity Stress System TNF gene Testing Time Tubulin Vimentin Viral bisulfite sequencing cytokine endoplasmic reticulum stress inhibitor mesenchymal stromal cell mimetics novel plasma cell development programs protein protein interaction senescence single cell analysis single-cell RNA sequencing stressor synergism whole genome

项目摘要

Project 3: Role of Cellular Senescence in long-lived plasma cell generation Abstract: Human long-lived plasma cells (LLPC) are important in the maintenance of protective serum antibodies. The mechanisms of how LLPC are generated and sustained are not well elucidated. These bone marrow resident cells are terminally differentiated, nondividing, and refractory to apoptosis yet they are capable of secreting antibodies for a lifetime. Exodus from cell cycle and resistance to apoptosis are major hallmarks of cellular senescence. In this application, we will understand the role of cellular senescence in LLPC maturation transcriptionally and epigenetically through single cell analysis. We will also dissect the unique senescence pathways that are needed for survival; then finally we will identify the unique components of the BM microniche that plays a role in survival and senescence.

项目3：细胞衰老在长寿命浆细胞生成中的作用摘要：人长寿命浆细胞(LLPC)在维持保护性血清中起重要作用抗体。LLPC是如何产生和维持的机制还没有得到很好的阐明。这些骨髓驻留细胞是终末分化的，不能分裂，不能细胞凋亡，但它们能够终生分泌抗体。脱离细胞周期和细胞周期对凋亡的抵抗是细胞衰老的主要标志。在此应用程序中，我们将从转录和表观遗传学上理解细胞衰老在LLPC成熟中的作用通过单细胞分析。我们还将剖析独特的衰老途径生存所需；然后最后我们将确定BM微粒子的独特组件对生存和衰老起着重要作用。