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Human IgE Antibody Secreting Cells in Blood, Nasal Polyps, and Bone Marrow

血液、鼻息肉和骨髓中的人类 IgE 抗体分泌细胞

基本信息

批准号：
8779709
负责人：
Frances Eun-Hyung Lee
金额：
$ 19.5万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-12-04 至 2015-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8779709
关键词：
Acute Acute-Phase Reaction Adult Affect Allergens Allergic Allergic Disease American Antibodies Antibody Repertoire Antibody Response Antigens Asthma B-Lymphocytes Basophils Binding Biological Assay Biological Response Modifiers Blood Bone Marrow CD19 gene Cell model Cells Child Collaborations Data Derivation procedure Discrimination Disease Extrinsic asthma FDA approved Generations Half-Life Health Human Human Biology Hymenoptera Hypersensitivity IgE IgG1 IgG4 Immune Immunoglobulin G Immunoglobulin-Secreting Cells Individual Insect Sting Laboratories Lead Life Maintenance Measures Mediator of activation protein Memory Memory B-Lymphocyte Modeling Mus Nasal Polyps Natural History Patients Phase Plasma Cells Population Positioning Attribute Prevalence Property Proteomics Regulation Reporter Serum Signal Transduction Source Structure of germinal center of lymph node Technology Testing Therapeutic Time Vaccines Venoms asthmatic patient base cohort cost high risk mast cell mouse model next generation sequencing novel pathogen response tool

项目摘要

DESCRIPTION (provided by applicant): Allergies affect over one third of the world's population, and the prevalence of asthma is estimated at 300 million patients worldwide 17. In the US alone, asthma prevalence is 8-10% with a subset of 10% who suffer from severe disease. Allergen-specific IgE is a major mediator of severe allergic conditions and asthma exacerbations, but little is understood about the biology of human IgE in these patients. The presence of human IgE long-lived plasma cells is a matter of controversy, since mouse models show little evidence of IgE BM resident plasma cells fueling debate that IgE levels are maintained only by ongoing replenishment from short-lived ASC. In my laboratory, we have identified a new LLPC subset in the human bone marrow (BM) compartment and characterized 5 novel circulating ASC populations. In this application, we will demonstrate the presence of human IgE BM LLPC in allergic patients and understand the cellular origins of allergen-specific IgE responses during acute recall responses (after natural allergen exposures) in blood, bone marrow and nasal polyps.

描述（由申请人提供）：过敏症影响世界上三分之一以上的人口，估计全世界有3亿哮喘患者17。仅在美国，哮喘患病率为8-10%，其中10%的人患有严重疾病。过敏原特异性IgE是严重过敏性疾病和哮喘恶化的主要介质，但对这些患者中人类IgE的生物学知之甚少。人IgE长寿浆细胞的存在是一个有争议的问题，因为小鼠模型显示IgE BM驻留浆细胞的证据很少，这引发了关于IgE水平仅通过短期ASC的持续补充来维持的争论。在我的实验室中，我们在人骨髓（BM）隔室中鉴定了一个新的LLPC亚群，并表征了5个新的循环ASC群体。在本申请中，我们将证明过敏患者中存在人IgE BM LLPC，并了解血液、骨髓和鼻息肉中急性回忆反应（自然过敏原暴露后）期间过敏原特异性IgE反应的细胞起源。