Human IgE Antibody Secreting Cells in Blood, Nasal Polyps, and Bone Marrow

血液、鼻息肉和骨髓中的人类 IgE 抗体分泌细胞

• 批准号: 8630264
• 负责人: Frances Eun-Hyung Lee
• 金额: $ 23.4万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-12-04 至 2015-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8630264
• 关键词: Acute; Acute-Phase Reaction; Adult; Affect; Allergens; Allergic; Allergic Disease; American; Antibodies; Antibody Formation; Antibody Repertoire; Antigens; Asthma; B-Lymphocytes; Basophils; Binding; Biological Assay; Biological Response Modifiers; Blood; Bone Marrow; CD19 gene; Cell model; Cells; Child; Collaborations; Data; Derivation procedure; Discrimination; Disease; Extrinsic asthma; FDA approved; Generations; Half-Life; Human; Human Biology; Hymenoptera; Hypersensitivity; IgE; IgG1; IgG4; Immune; Immunoglobulin G; Immunoglobulin-Secreting Cells; Individual; Insect Sting; Laboratories; Lead; Life; Maintenance; Measures; Mediator of activation protein; Memory; Memory B-Lymphocyte; Modeling; Mus; Nasal Polyps; Natural History; Patients; Phase; Plasma Cells; Population; Positioning Attribute; Prevalence; Property; Proteomics; Regulation; Reporter; Serum; Signal Transduction; Source; Structure of germinal center of lymph node; Technology; Testing; Therapeutic; Time; Vaccines; Venoms; asthmatic patient; base; cohort; cost; high risk; mast cell; mouse model; next generation sequencing; novel; pathogen; public health relevance; response; tool

Abstract: Allergies affect over one third of the world's population, and the prevalence of asthma is estimated at 300 million patients worldwide 17. In the US alone, asthma prevalence is 8-10% with a subset of 10% who suffer from severe disease. Allergen-specific IgE is a major mediator of severe allergic conditions and asthma exacerbations, but little is understood about the biology of human IgE in these patients. The presence of human IgE long-lived plasma cells is a matter of controversy, since mouse models show little evidence of IgE BM resident plasma cells fueling debate that IgE levels are maintained only by ongoing replenishment from short-lived ASC. In my laboratory, we have identified a new LLPC subset in the human bone marrow (BM) compartment and characterized 5 novel circulating ASC populations. In this application, we will demonstrate the presence of human IgE BM LLPC in allergic patients and understand the cellular origins of allergen-specific IgE responses during acute recall responses (after natural allergen exposures) in blood, bone marrow and nasal polyps.

摘要： 过敏症影响着世界上三分之一以上的人口，哮喘的患病率估计为300 全球17万名患者仅在美国，哮喘患病率为8-10%，其中10%的人患有哮喘。 严重的疾病。过敏原特异性IgE是严重过敏性疾病和哮喘的主要介质 急性加重，但对这些患者中人IgE的生物学知之甚少。的存在 人类IgE长寿浆细胞是一个有争议的问题，因为小鼠模型显示IgE的证据很少 BM驻留浆细胞引发了争论，即IgE水平仅通过持续补充维持， 短寿命ASC。在我的实验室中，我们已经在人骨髓（BM）中鉴定出一个新的LLPC亚群。 隔室和表征5个新的循环ASC群体。在本应用程序中，我们将演示 过敏患者中存在人IgE BM LLPC，并了解过敏原特异性LLPC的细胞起源。 血液、骨髓和血液中急性回忆反应（自然过敏原暴露后）期间的IgE反应 鼻息肉