Human IgE Antibody Secreting Cells in Blood, Nasal Polyps, and Bone Marrow

摘要： 过敏症影响着世界上三分之一以上的人口，哮喘的患病率估计为300 全球17万名患者仅在美国，哮喘患病率为8-10%，其中10%的人患有哮喘。 严重的疾病。过敏原特异性IgE是严重过敏性疾病和哮喘的主要介质 急性加重，但对这些患者中人IgE的生物学知之甚少。的存在 人类IgE长寿浆细胞是一个有争议的问题，因为小鼠模型显示IgE的证据很少 BM驻留浆细胞引发了争论，即IgE水平仅通过持续补充维持， 短寿命ASC。在我的实验室中，我们已经在人类骨髓（BM）中鉴定出一个新的LLPC亚群。 隔室和特征的5个新的循环ASC群体。在本应用程序中，我们将演示 过敏患者中存在人IgE BM LLPC，并了解过敏原特异性LLPC的细胞起源。 血液、骨髓和血液中急性回忆反应（自然过敏原暴露后）期间的IgE反应 鼻息肉

Abstract: Allergies affect over one third of the world's population, and the prevalence of asthma is estimated at 300 million patients worldwide 17. In the US alone, asthma prevalence is 8-10% with a subset of 10% who suffer from severe disease. Allergen-specific IgE is a major mediator of severe allergic conditions and asthma exacerbations, but little is understood about the biology of human IgE in these patients. The presence of human IgE long-lived plasma cells is a matter of controversy, since mouse models show little evidence of IgE BM resident plasma cells fueling debate that IgE levels are maintained only by ongoing replenishment from short-lived ASC. In my laboratory, we have identified a new LLPC subset in the human bone marrow (BM) compartment and characterized 5 novel circulating ASC populations. In this application, we will demonstrate the presence of human IgE BM LLPC in allergic patients and understand the cellular origins of allergen-specific IgE responses during acute recall responses (after natural allergen exposures) in blood, bone marrow and nasal polyps.