Zeiss Lightsheet 7
蔡司 Lightsheet 7
基本信息
- 批准号:10430494
- 负责人:
- 金额:$ 59.72万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-04-01 至 2024-03-31
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalAreaAwarenessBrain regionCardiologyClinicalCollaborationsCommunitiesConfocal MicroscopyCore FacilityDevelopmental BiologyEmbryonic HeartEnsureFosteringFundingFutureHistopathologyImageInfrastructureInstitutionInvestmentsLightMicrofluidicsMicroscopeMinorMolecularNeurologyOtolaryngologyPhotobleachingPhototoxicityResearchResearch PersonnelResolutionResourcesSamplingSystemTimeTissuesUnited States National Institutes of HealthUniversitiesbaseclinical applicationimaging systeminstrumentinstrumentationlight microscopymedical schoolsmicroscopic imagingoperationprogramsprostate biopsysuccesstemporal measurementvirologyward
项目摘要
Project Summary
The School of Medicine’s Light Microscopy Imaging Core (SOM-LMIC) at Case Western Reserve University
requests funding for a Zeiss Lightsheet 7 microscope, which would have an enormous benefit to our active
research community. Currently, the Cleveland area does not have a commercial light-sheet microscope (LSM)
available to its researchers. This is particularly surprising considering the excitement and increased use of LSM.
Obtaining this imaging system would allow us to continue to provide state-of-the-art imaging to our expanding
user base. The Zeiss Lightsheet 7 provides several advantages that our current instrumentation cannot provide,
allowing our current users to ask new questions as well as attracting new projects we currently cannot support.
First, the system enables one to acquire cellular resolution over sizeable fields of view. Many of our users have
struggled to assess the spatial extent of molecular changes over larger regions (e.g., entire brain region,
throughout embryonic heart, prostate biopsy, etc.). Second, the Zeiss Lightsheet 7 has much higher acquisition
rates than standard confocal microscopy. Because an entire sheet is collected in comparison to point by point
for confocal, the acquisition rates are much higher. This will benefit all of our users by enabling them to either
save time, realistically image larger samples for a study, or provide higher temporal resolution for live imaging.
Third, several of our users have problems with photobleaching or phototoxicity (e.g., live imaging applications).
The Zeiss Lightsheet 7 only illuminates tissue that is being imaged which greatly reduces the required light levels
and photobleaching/phototoxicity compared to confocal microscopy. The Zeiss Lightsheet 7 will ensure the
continued success of many NIH-funded programs at CWRU and nearby institutions. For this proposal, we have
identified 10 major and 4 minor users. This imaging system will allow our users to make contributions to a diverse
number of fields including developmental biology (Atit, Bearer, Jenkins, Ward), 3D histopathology (Gupta,
Madabhushi), virology (Levine), otolaryngology (McDermott), subcellular dynamics (Mears, Kisley), cardiology
(Rollins), neurology (Tesar, Pieper), and clinical applications of microfluidics (Gurkan). We anticipate that this
instrument will foster collaborations within our user groups and that other users will benefit from this resource
especially after they become aware of its potential. Without the Zeiss Lightsheet 7 many of the projects described
in this proposal will not be possible. As one of Case Western Reserve University’s 11 Institutional Core Facilities,
significant investment into administration and infrastructure has been made to support our daily operations. To
support the long-term operation of this instrument, the University has agreed to further contribute nearly 130k.
The requested Zeiss Lightsheet 7 is essential to the needs of our evolving research, clinical and translational
programs and will expand the range of viable projects in the future.
项目摘要
凯斯西储大学医学院光学显微成像核心(SOM-LMIC)
要求为蔡司Lightsheet 7显微镜提供资金,这将对我们的积极工作产生巨大的好处。
研究社区。目前,克利夫兰地区还没有商用光片显微镜(LSM)
提供给研究人员。考虑到LSM的兴奋和增加的使用,这特别令人惊讶。
获得这一成像系统将使我们能够继续提供最先进的成像,我们的扩大
用户基础。蔡司Lightsheet 7提供了我们目前的仪器无法提供的几个优点,
允许我们现有的用户提出新的问题,并吸引我们目前无法支持的新项目。
首先,该系统使人们能够在相当大的视场内获得细胞分辨率。我们的许多用户
努力评估较大区域上分子变化的空间范围(例如,整个大脑区域,
整个胚胎心脏、前列腺活检等)。其次,蔡司Lightsheet 7的收购率要高得多,
比标准的共聚焦显微镜。因为与逐点收集相比,
对于共焦,获取速率要高得多。这将使我们的所有用户受益,使他们能够
节省时间,为研究提供更大样本的逼真图像,或为实时成像提供更高的时间分辨率。
第三,我们的一些用户有光漂白或光毒性问题(例如,实时成像应用)。
蔡司Lightsheet 7仅照亮正在成像的组织,这大大降低了所需的光水平
和光漂白/光毒性。蔡司Lightsheet 7将确保
在CWRU和附近机构的许多NIH资助的项目的持续成功。对于这个建议,我们有
确定了10个主要用户和4个次要用户。这一成像系统将使我们的用户作出贡献,
包括发育生物学(Atit,Bearer,Jenkins,Ward),3D组织病理学(Gupta,
Madabhushi)、病毒学(Levine)、耳鼻喉科(McDermott)、亚细胞动力学(米尔斯、Kisley)、心脏病学
(Rollins)、神经学(Tesar,Pieper)和微流体的临床应用(Gurkan)。我们预计,
工具将促进我们的用户组内的合作,其他用户将从这一资源中受益
尤其是在他们意识到它的潜力之后。没有蔡司Lightsheet 7,许多项目描述
在这个提议中是不可能的。作为凯斯西储大学的11个机构核心设施之一,
我们在行政和基础设施方面作出了大量投资,以支持我们的日常运作。到
为了支持该仪器的长期运行,该大学已同意进一步捐款近13万美元。
所要求的蔡司Lightsheet 7对我们不断发展的研究、临床和翻译需求至关重要
计划,并将在未来扩大可行项目的范围。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MICHAEL W. JENKINS其他文献
MICHAEL W. JENKINS的其他文献
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{{ truncateString('MICHAEL W. JENKINS', 18)}}的其他基金
Understanding neural control of the ocular surface
了解眼表的神经控制
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10586931 - 财政年份:2022
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$ 59.72万 - 项目类别:
Understanding neural control of the ocular surface
了解眼表的神经控制
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10707246 - 财政年份:2022
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Infrared Neuromodulation Reveals a New Understanding of Ganglion Organization
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9513867 - 财政年份:2017
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Infrared Neuromodulation Reveals a New Understanding of Ganglion Organization
红外神经调节揭示了对神经节组织的新认识
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10004289 - 财政年份:2017
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Infrared Neuromodulation Reveals a New Understanding of Ganglion Organization
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9930180 - 财政年份:2017
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Optical Tools to Assess the Role of Cardiac Function in the Development of Congenital Heart Defects
评估心脏功能在先天性心脏缺陷发展中的作用的光学工具
- 批准号:
10593074 - 财政年份:2015
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Optical Tools to Assess the Role of Cardiac Function in the Development of Congenital Heart Defects
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8985102 - 财政年份:2015
- 资助金额:
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Optical Tools to Assess the Role of Cardiac Function in the Development of Congenital Heart Defects
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10211096 - 财政年份:2015
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$ 59.72万 - 项目类别:
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