Dissemination of FlyWire, A Whole-Brain Connectomics Resource

全脑连接组学资源 FlyWire 的传播

• 批准号: 10439970
• 负责人: Mala Murthy
• 金额: $ 118.92万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2027-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10439970
• 关键词: Address; Adult; BRAIN initiative; Behavior; Biological Models; Brain; Brain region; Cells; Communities; Computers; Conflict (Psychology); Data; Data Set; Development; Drosophila genus; Drosophila melanogaster; Educational workshop; Electrons; Female; Funding; Genetic; Gold; Grant; Image; Incentives; Infrastructure; Internet; Mammals; Manuals; Mediating; Neuraxis; Neuroanatomy; Neurons; Neurosciences; Outcome; Persons; Physiology; Publications; Research; Resources; Running; Scientist; Sex Differences; Structure; Time; Training; Update; Visualization; automated segmentation; base; connectome; deep learning; design; fly; innovation; interest; learning strategy; male; member; microscopic imaging; neural circuit; online community; online tutorial; outreach; prevent; reconstruction; recruit; skills; tool

This proposal will disseminate FlyWire, a Drosophila whole brain connectomics resource. We used advances in AI to segment all neurons from a whole brain EM volume called FAFB. The automated segmentation is of high enough quality that, in combination with innovative proofreading tools, scientists can relatively quickly proofread circuits of interest. The community of current collaborators includes about 160 scientists from 40 labs, who have so far succeeded at proofreading more than 15% of the neurons in the fly brain. Several publications have resulted, and more are on the way. Proofreading is required because automated segmentation, while good, contains errors that must be fixed with manual correction. Janelia/HHMI spent 50 person-years proofreading an EM volume that contains roughly one-third of the neurons in another female brain, a resource called the hemibrain. A whole brain connectome, in contrast, will make it possible to address questions about brain-wide circuit organization and about any brain region. We created this unique resource with a three-year grant from the BRAIN Initiative, and are now seeking additional funding to disseminate the resource more widely. Our framework for dissemination will set the standard for emerging, larger connectomics datasets, such as those of mammals. We propose three Aims for dissemination that include outreach efforts, deploying a centrally-managed proofreading team, and enhancing the user interface and tools available to access FlyWire data. In particular, a Community Manager will organize the community of scientists working in FlyWire, will recruit and train new users, will run workshops, and will coordinate access to the trained proofreaders. Scientists can apply to have an expert proofreader assist with their circuit reconstruction projects, and we will give priority to new users. Finally, in the first year of the project we will implement GUI tools in FlyWire that facilitate discovery without necessitating programming skills.

该提案将传播 FlyWire，一种果蝇全脑连接组学资源。我们 利用人工智能的进步从称为 FAFB 的全脑 EM 体积中分割所有神经元。这 自动分割具有足够高的质量，结合创新 校对工具，科学家可以相对快速地校对感兴趣的电路。社区 目前的合作者包括来自 40 个实验室的约 160 名科学家，他们迄今为止已取得了成功 校对果蝇大脑中超过 15% 的神经元。多个出版物已 结果，更多的事情正在发生。需要校对，因为自动分割， 虽然很好，但包含必须通过手动更正来修复的错误。 Janelia/HHMI 花费了 50 人年校对包含大约三分之一神经元的 EM 卷 另一个女性大脑，一种称为半脑的资源。相比之下，全脑连接组 将使解决有关全脑电路组织和任何其他问题的问题成为可能 大脑区域。我们利用 BRAIN Initiative 的三年资助创建了这一独特的资源， 目前正在寻求额外资金以更广泛地传播该资源。我们的 传播框架将为新兴的、更大的连接组学数据集设定标准， 例如哺乳动物的那些。我们提出了三个传播目标，其中包括外展 努力，部署集中管理的校对团队，并增强用户界面和 可用于访问 FlyWire 数据的工具。特别是，社区经理将组织 在 FlyWire 工作的科学家社区将招募和培训新用户，举办研讨会， 并将协调对经过培训的校对人员的访问。科学家可以申请聘请专家 校对人员协助他们进行电路改造项目，我们将优先考虑新用户。 最后，在项目的第一年，我们将在 FlyWire 中实现 GUI 工具，以方便 无需编程技能即可发现。