Dissemination of FlyWire, A Whole-Brain Connectomics Resource

全脑连接组学资源 FlyWire 的传播

• 批准号: 10668452
• 负责人: Mala Murthy
• 金额: $ 123.73万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2027-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10668452
• 关键词: Address; Adult; BRAIN initiative; Behavior; Biological Models; Brain; Brain region; Cells; Central Nervous System; Collaborations; Communities; Computers; Data; Data Set; Development; Drosophila genus; Drosophila melanogaster; Educational workshop; Electrons; Female; Funding; Genetic; Gold; Grant; Image; Incentives; Infrastructure; Internet; Mammals; Manuals; Mediating; Neuroanatomy; Neurons; Neurosciences; Outcome; Persons; Physiology; Publications; Research; Resources; Running; Scientist; Sex Differences; Structure; Time; Training; Update; Visualization; automated segmentation; community organizations; connectome; connectome data; deep learning; design; fly; innovation; interest; learning strategy; male; member; microscopic imaging; neural circuit; online community; online tutorial; outreach; prevent; reconstruction; recruit; skills; tool

This proposal will disseminate FlyWire, a Drosophila whole brain connectomics resource. We used advances in AI to segment all neurons from a whole brain EM volume called FAFB. The automated segmentation is of high enough quality that, in combination with innovative proofreading tools, scientists can relatively quickly proofread circuits of interest. The community of current collaborators includes about 160 scientists from 40 labs, who have so far succeeded at proofreading more than 15% of the neurons in the fly brain. Several publications have resulted, and more are on the way. Proofreading is required because automated segmentation, while good, contains errors that must be fixed with manual correction. Janelia/HHMI spent 50 person-years proofreading an EM volume that contains roughly one-third of the neurons in another female brain, a resource called the hemibrain. A whole brain connectome, in contrast, will make it possible to address questions about brain-wide circuit organization and about any brain region. We created this unique resource with a three-year grant from the BRAIN Initiative, and are now seeking additional funding to disseminate the resource more widely. Our framework for dissemination will set the standard for emerging, larger connectomics datasets, such as those of mammals. We propose three Aims for dissemination that include outreach efforts, deploying a centrally-managed proofreading team, and enhancing the user interface and tools available to access FlyWire data. In particular, a Community Manager will organize the community of scientists working in FlyWire, will recruit and train new users, will run workshops, and will coordinate access to the trained proofreaders. Scientists can apply to have an expert proofreader assist with their circuit reconstruction projects, and we will give priority to new users. Finally, in the first year of the project we will implement GUI tools in FlyWire that facilitate discovery without necessitating programming skills.

这项提议将传播Flywire，一种果蝇全脑连接的资源。我们 利用人工智能的进步，从被称为FAFB的整个大脑EM体积中分割出所有神经元。这个 自动分割的质量足够高，与创新的 校对工具中，科学家可以相对快速地校对感兴趣的电路。社区 目前的合作者包括来自40个实验室的大约160名科学家，他们到目前为止已经成功 在校对苍蝇大脑中超过15%的神经元。几种出版物都有 结果，还有更多的项目正在进行中。校对是必需的，因为自动分割， 虽然很好，但包含必须通过手动更正修复的错误。Janelia/HHMI花费了50 人年校对一本EM卷，其中包含大约三分之一的神经元 另一种女性大脑，一种被称为半脑的资源。相比之下，整个大脑的连接体， 将有可能解决关于全脑回路组织和关于任何 大脑区域。我们用大脑计划提供的为期三年的拨款创造了这一独特的资源， 现在正在寻求额外的资金，以更广泛地传播资源。我们的 传播框架将为新兴的、更大的连接学数据集设定标准， 比如哺乳动物的。我们提出了包括外展在内的三个传播目标 努力，部署一个集中管理的校对团队，并增强用户界面和 可用于访问飞线数据的工具。特别是，一名社区经理将组织 在Flywire工作的科学家社区，将招募和培训新用户，将举办研讨会， 并将协调与训练有素的校对员的接触。科学家可以申请聘请一名专家 校对员协助他们的电路改造项目，我们将优先考虑新用户。 最后，在项目的第一年，我们将在Flywire中实现图形用户界面工具，以促进 无需具备编程技能即可进行发现。