CRISPR-based pathway activation for bioactive molecule discovery in fungi

基于 CRISPR 的途径激活用于发现真菌中的生物活性分子

• 批准号: DP210102180
• 负责人: A/Prof Yit-Heng Chooi
• 金额: $ 38.22万
• 依托单位: The University of Western Australia
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-01-18 至 2024-03-08
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP210102180
• 关键词: CRISPR; based; pathway; activation; bioactive

Fungi produce an incredible array of unique bioactive molecules, many of which have contributed greatly to humanity (e.g. the antibiotic penicillin, which has saved millions of lives since its discovery). DNA sequencing has revealed many fungi contain the genetic instructions to produce new molecules that have not been seen previously. However, these genes are “switched off" by default and cannot be accessed. This project will develop innovative new methods to "hot-wire" these genes, allowing them to turn on and produce a treasure trove of new bioactive molecules. The outcomes of this project will transform our abilities to tap into the hidden potential of fungi to generate new lead molecules for the agricultural and medical industries.

真菌产生了一系列令人难以置信的独特生物活性分子，其中许多对人类做出了巨大贡献（例如抗生素青霉素，自发现以来已挽救了数百万人的生命）。DNA测序显示，许多真菌含有产生以前从未见过的新分子的遗传指令。然而，这些基因在默认情况下是“关闭”的，无法访问。该项目将开发创新的新方法来“热线”这些基因，使它们能够打开并产生新的生物活性分子的宝库。该项目的成果将改变我们挖掘真菌隐藏潜力的能力，为农业和医疗行业产生新的铅分子。