MHC Genetic Typing

MHC基因分型

• 批准号: 10447032
• 负责人: CARLOS A SARIOL
• 金额: $ 28.18万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10447032
• 关键词: AIDS Vaccines; Acquired Immunodeficiency Syndrome; Address; Alleles; Animal Husbandry; Animals; Arizona; Back; Biochemistry; Biological Models; Biomedical Research; Breeding; Caribbean region; Caring; Cellular Immunity; Collaborations; Communities; DNA laboratory; Databases; Development; Disease; Disease Progression; Ensure; Excision; Foundations; Funding; Generations; Genetic; Genetic Heterogeneity; Genetic Variation; Genome; Goals; HIV; Health; Heterozygote; Human; Immune; Immune response; Inbreeding; Individual; Institution; International; Island; Macaca; Macaca mulatta; Maintenance; Major Histocompatibility Complex; Modeling; Monkeys; Natural Sciences; Physiological; Physiology; Population; Population Genetics; Primates; Production; Proteins; Protocols documentation; Research; Research Personnel; Research Project Grants; Resources; Roundup; SIV; Safety; Schools; Shapes; Stratification; Testing; Time; United States National Institutes of Health; Universities; Virus; animal colony; base; computerized; design; driving force; genetic analysis; genetic pedigree; germ free condition; human disease; human subject; improved; intergenerational; mathematical sciences; microbicide; nonhuman primate; operation; programs; relational database; research and development; research facility; therapeutic development

PROJECT SUMMARY/ABSTRACT: The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. Genetic variation will be maintained in the SPF colony using the genetic management strategies described in this application. The genetic management approach will allow for enrichment of desired MHC markers in demand for biomedical research while maintaining genetic diversity. This specific core is designed to continue our current efforts to maintain a genetically diverse, MHC-defined SPF Indian-origin rhesus macaque colony for use in AIDS-related research. In 2015, the KDL began its collaboration with the current U42 to design and improve the SPF breeding colony to enhance the efficiency of colony maintenance as well as to maximize genetic heterogeneity of the colony animals. Since then we have been constructing multigenerational SPF pedigrees at the CPRC which can be traced back to founders in the Cayo Santiago island population.

项目总结/摘要： 这项申请的主要目标是维持加勒比地区的无特定病原体（SPF）计划 灵长类动物研究中心（CPRC）通过支持运营，管理，兽医护理，病毒学 和MHC分型，并通过改进我们的综合遗传管理计划。的独特特征 CPRC动物有利于成功建立和可持续的SPF群体，这些动物是 天然不含SIV和SRV-D，这是四种待检测病毒中的两种。然而，为了安全和必要的 对于SPF计划，我们继续系统地检测所有四种病毒（B病毒、STLV、SIV和SRV-D）。的 计划提供具有明确遗传和病毒学背景的印度恒河猴。 群体遗传分析显示，SPF群体随着时间的推移保持遗传同质性 具有足够量的杂合性和来自其非SPF建立者的最小分层。遗传 使用本说明书中描述的遗传管理策略， 应用程序.遗传管理方法将允许富集所需的MHC标记物 生物医学研究，同时保持遗传多样性。这个特殊的核心是为了继续我们目前的 努力维持遗传多样性的、MHC定义的SPF印度恒河猴群体，用于 艾滋病相关研究。2015年，KDL开始与目前的U42合作，设计和改进 SPF育种集落，以提高集落维护效率以及最大限度地提高遗传 殖民地动物的异质性。从那时起，我们一直在构建多代SPF谱系， 可追溯到圣地亚哥岛人口中的创始人。