ENHANCEMENT OF THE CPRC-SPF RHESUS MONKEY PROGRAM

加强 CPRC-SPF 恒河猴计划

• 批准号: 8502561
• 负责人: CARLOS A SARIOL
• 金额: $ 236.79万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8502561
• 关键词: Acquired Immunodeficiency Syndrome; Animal Housing; Animals; Area; Biological Assay; Biomedical Research; Birth; Breeding; Caribbean region; Characteristics; Communities; Country; Development; Funding; Genetic; Genetic Heterogeneity; Genetic Markers; Grant; Growth; Housing; Human; Inbreeding; Macaca; Macaca mulatta; Medical; Modeling; Monitor; Monkeys; National Center for Research Resources; Papovaviridae; Polyomavirus; Primates; Provider; Puerto Rico; Reproduction; Research; Research Personnel; Research Project Grants; Resources; SIV; Science; Serologic tests; Serological; Simian T-lymphotropic virus 1; Simian virus 40; Specific Pathogen Frees; Structure; Surveys; Techniques; Testing; United States National Institutes of Health; Universities; Virus; cohort; germ free condition; meetings; offspring; programs; vector vaccine; virology

DESCRIPTION (provided by applicant): The objective of this proposal is to maintain and enhance the specific pathogen-free (SPF) rhesus macaque supply and breeding colony program at the Caribbean Primate Research Center (CPRC). This program consists of two SPF colonies (one closed and the other open). While the closed colony growths only by births, the open colony increases its size not only by births, but also by incorporating SPF candidates to its cohort. The closed colony (referred as to the U24 SPF colony) will consist of a minimum of 652 animals, and the open colony (referred as to SPF-A) will start with 468 animals. Thus, the founding stock of these genetically-characterized Indian-origin monkey colonies will be donated by CPRC and will consist of approximately 1,120 SPF (Herpes B, STLV-1, SIV and SRV-free) rhesus macaques. About 82 (7%) of the U24 SPF animals will be also free of the papovavirus SV40. Thus, this program will consist of two U24 SPF sub colonies: one SV40-free and the other SV40 positive. Animals from these SPF sub colonies will be maintained in well-defined and separated areas, will be housed in reproduction corncribs, and will be quarterly tested for the presence of the above mentioned target viruses. All SPF colonies will be closely monitored by well-established genetic and virology techniques and assays. A panel of highly polymorphic genetic markers will be used to characterize the U24 SPF and SPF-A colonies and to optimize the structure of breeding groups, maximizing the level of their genetic heterogeneity. At the end of this four year grant, the SPF-A colony will be closed. The entire program will produce a total estimated offspring of more than 1,350 SPF rhesus macaques, of which 96 will be also SV40 negative. It is expected that the SPF colonies of this program will make available to PHS-funded grants about 300 animals/year and that it will attain self-sufficiency by the end of this grant. CPRC SPF macaques will help meet the increased research demand that exists for these animals. Our Center will continue to make significant contributions to AIDS and to other research programs by providing high quality, healthy, and genetically-characterized SPF rhesus monkeys to NIH-sponsored research initiatives.

描述（由申请人提供）：本提案的目的是维持和加强加勒比灵长类动物研究中心（CPRC）的特定无病原体（SPF）恒河猴供应和繁殖群体计划。该计划由两个SPF菌落（一个封闭，另一个开放）组成。封闭的蜂群只通过出生增加，而开放的蜂群不仅通过出生增加，而且通过将SPF候选者纳入其队列来增加其规模。封闭群体（称为U24 SPF群体）将至少由652只动物组成，开放群体（称为SPF- a）将从468只动物开始。因此，这些具有遗传特征的印度猕猴群体的创始种群将由CPRC捐赠，并将由大约1120只SPF （B型疱疹、STLV-1型疱疹、SIV和无srv）恒河猴组成。大约82只（7%）的U24 SPF动物也将不含hpv SV40病毒。因此，该程序将由两个U24 SPF亚菌落组成：一个不含SV40，另一个SV40阳性。来自这些SPF亚群的动物将被饲养在明确和隔离的区域，将被安置在繁殖玉米笼中，并将每季度检测上述目标病毒的存在。所有SPF菌落将通过完善的遗传和病毒学技术和分析密切监测。一组高度多态性的遗传标记将用于表征U24 SPF和SPF-A群体，并优化育种群体的结构，最大限度地提高其遗传异质性水平。在四年拨款结束时，SPF-A种群将被关闭。整个计划将产生超过1,350只SPF猕猴的后代，其中96只也将是SV40阴性。预计该计划的SPF菌落每年将为phs资助的赠款提供约300只动物，并在赠款结束时实现自给自足。CPRC SPF猕猴将有助于满足对这些动物日益增长的研究需求。我们中心将继续为艾滋病和其他研究项目做出重大贡献，为nih资助的研究项目提供高质量、健康和具有遗传特征的SPF恒河猴。