Ion Fountain Nanopore Readers for High-Resolution DNA and RNA Sequencing
Ion Fountain Nanopore Readers for High-Resolution DNA and RNA Sequencing
批准号:
10448254
负责人:
Meni Wanunu
金额:
$29.94万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
已结题
起止时间:
2021-07-15 至 2023-06-30
关键词:
ArchitectureAreaBackBiological SciencesCaliberCationsChemicalsComplexComputer softwareDNADNA Modification ProcessDNA SequenceDNA biosynthesisDNA sequencingDetectionDevelopmentDevicesDiseaseElectrolytesElectronicsEpigenetic ProcessExhibitsFilmGenetic DiseasesGenomic DNAGenomic SegmentGenomicsGoldImmobilizationIonsKineticsLongevityMalignant NeoplasmsMeasurementMeasuresMediatingMembraneMethodsModificationMonitorMosaicismNetwork-basedNucleic acid sequencingNucleotidesOligonucleotidesOpticsOrganismPhasePhysiologic pulsePlayProductionRNARNA SequencesReaderResistanceResolutionRoleSideSignal TransductionSilanesSingle-Stranded DNASodium ChlorideSystemTechnologyThickThinnessTimeTrainingVariantbasecostdesigndetection sensitivityds-DNAelectrical propertyexperimental studyhelicasehydrophilicityimprovedintercalationinterstitiallink proteinmonolayernanoporeneural networkphosphonatereal time monitoringreference genomesensorsilicon nitridesingle moleculesingle molecule real time sequencingsolid statetranscriptome sequencingtwo-dimensionalvoltage
中文摘要
项目摘要
我们建议开发一种新的范例,在纳米孔为基础的传感,涉及离子喷泉纳米孔读者
(IFNRs)。在这些设备中,DNA或RNA分子通过一个亚2纳米的孔移动,
通过记录离子在主体和主体之间来回摆动的速率,
膜内包含孔的非常小的空间体积。该装置的一个关键部件是
与MXene相关,MXene是导电的、亲水的、且大多数
重要的是,在其片层之间的间隙区域插入各种阳离子。我们最近
开发了一种用于单层和双层MXene片材的晶圆级组装的方法,其中MXene薄片
自发组织成导电大面积有序膜的镶嵌体。通过制造一个双层
MXene薄膜上有一个直径小于2纳米的孔,我们创造了一个离子喷泉,可以储存和释放离子
通过使它们穿过孔开口。这种类型的纳米孔传感器的主要优点是,
在孔的一侧消除了进入阻力,这超过了
传统的纳米孔传感。这种类型的装置的另一个优点是物理上较厚的孔可以
同时仍然表现出原子级薄的纳米孔膜的分辨率。我们提议
在这里开发IFNR架构,补充电子测量平台,允许DNA和
RNA捕获和读出,以及碱基判定软件。如果成功,我们的设备将能够读取
DNA/RNA比最先进的技术更快(每秒> 1,000个碱基),而且,
各种类型的DNA和RNA碱基修饰的准确性。
英文摘要
Project Summary
We propose to develop a new paradigm in nanopore-based sensing, involving ion-fountain nanopore readers
(IFNRs). In these devices, a DNA or RNA molecule is moved through a sub-2-nm pore, and electronic sensing
of the DNA sequence is made by recording the rate at which ions floss back and forth between the bulk and
the very small volume of space within the membrane that contains the pore. A key component of the device is
related to MXenes, two-dimensional layered materials that are electrically conductive, hydrophilic, and most
importantly, intercalate various cations in the interstitial region between their sheets. We have recently
developed a method for waferscale assembly of monolayer and bilayer MXene sheets in which MXene flakes
spontaneously organize to a mosaic of electrically conductive large-area ordered films. By making a bilayer
MXene film with a sub-2-nm diameter hole through it, we create an ion fountain that stores and releases ions
by passing them through the pore opening. The main advantage of this type of a nanopore sensor is that
access resistance is eliminated on one side of the pore, which beats fundamental resolution limits of
conventional nanopore sensing. Another advantage of this type of a device is that a physically thicker pore can
be fabricated while still exhibiting a resolution of an atomically-thin nanopore membrane. We are proposing
here to develop the IFNR architecture, supplemental electronics measurement platform that allows DNA and
RNA capture and readout, and software for basecalling. If successful, our devices will be able to read
DNA/RNA faster than state-of-the-art technology (>1,000 bases per second) and further, detect at high
accuracy various types of DNA and RNA base modifications.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
DOI:
10.1002/smll.202105857
发表时间:
2022-03
期刊:
SMALL
影响因子:
13.3
作者:
[Mojtabavi, Mehrnaz, Tsai, Wan-Yu, VahidMohammadi, Armin, Zhang, Teng, Gogotsi, Yury, Balke, Nina, Wanunu, Meni]
通讯作者:
Wanunu, Meni
Ion Fountain Nanopore Readers for High-Resolution DNA and RNA Sequencing
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批准号:10204556
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项目类别:
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资助金额:$32.8万
-
财政年份:2021
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负责人:Meni Wanunu
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依托单位:
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批准号:10487746
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财政年份:2021
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依托单位:
Single-cell direct RNA sequencing using electrical zero-mode waveguides and engineered reverse transcriptases
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财政年份:2020
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负责人:Meni Wanunu
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依托单位:
Single-cell direct RNA sequencing using electrical zero-mode waveguides and engineered reverse transcriptases
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项目类别:
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资助金额:$62.92万
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负责人:Meni Wanunu
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依托单位:
Single-cell direct RNA sequencing using electrical zero-mode waveguides and engineered reverse transcriptases
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