Oxygen imaging by phosphorescence quenching

磷光猝灭氧气成像

• 批准号: 10448407
• 负责人: SERGEI VINOGRADOV
• 金额: $ 30.75万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-30 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10448407
• 关键词: 3-Dimensional; Adopted; Adoption; Area; Benchmarking; Bioenergetics; Biology; Blood Transfusion; Cell Culture Techniques; Cellular Metabolic Process; Chemistry; Clinic; Clinical; Collaborations; Communities; Computer software; Consultations; Consumption; Data Analyses; Data Collection; Disease; Documentation; Fiber Optics; Future; Grant; Health; Heart; Hematopoiesis; Image; Immunologics; Internet; Journals; Knowledge; Laboratories; Link; Measurement; Measures; Metabolism; Methods; Microscopic; Microscopy; Monitor; Names; National Institute of Neurological Disorders and Stroke; Nature; Neurosciences; Neurosciences Research; Ophthalmology; Oxygen; Oxygen saturation measurement; PUVA Photochemotherapy; Paper; Pathway interactions; Pennsylvania; Performance; Physiological; Play; Production; Protocols documentation; Publications; Radiation therapy; Recording of previous events; Research; Research Personnel; Resolution; Resources; Role; Running; Scanning; Site; Stroke; System; Techniques; Technology; Temperature; Time; Tissue Engineering; Tissue Therapy; Tissues; Training; Trauma; Tumor Biology; United States National Institutes of Health; Universities; Variant; Visit; Work; base; biological systems; biomedical imaging; bone engineering; cancer therapy; commercialization; data acquisition; experience; experimental study; instrument; interest; large scale production; luminescence; medical attention; medical schools; method development; minimally invasive; open source; phosphorescence; pre-clinical; programs; quantitative imaging; scale up; software development; stem cell biology; symposium; tissue oxygenation; tissue regeneration; tomography; tumor immunology; two-photon; web site

A Resource for Oxygen Imaging by Phosphorescence Quenching Project Summary Real-time, minimally invasive and spatially resolved measurements of tissue oxygenation have the potential to transform our understanding of many clinical problems, including those in tumor biology, management of stroke, ophthalmology, tissue regeneration, to name a few. Over the years our laboratory has developed a minimally invasive method for dynamic imaging of oxygen in biological systems, known as phosphorescence quenching oximetry. Recently, the method has been expanded by two-photon phosphorescence lifetime microscopy (2PLM), enabling dynamic imaging of oxygen gradients in tissues in 3D with micron-scale resolution, opening new horizons for research in neuroscience, stem cell biology, cancer immunology, tissue engineering and several other areas. Even more recently, the combination of phosphorescence with Cherenkov-Excited Luminescence Scanned Imaging (CELSI) enabled tomography of oxygen in 3D in pre-clinical setting in tissues undergoing radiation therapy. At the same time, phosphorescence-based oximetry is increasingly drawing attention from the medical community for its potential to directly evaluate physiologic status of tissue under trauma, optimize efficacy of blood transfusion as well as a marker in monitoring progress of photodynamic and radiation therapies. These and other applications have set the stage for broad dissemination of the phosphorescence technology across different biomedical fields. At the heart of the phosphorescence quenching method are special oxygen probes, developed and continuously optimized in our laboratory. The synthesis of these probes, including the newest and the most potent probe Oxyphor 2P, is not simple, and standard commercialization pathways are presently not feasible. Here we propose to establish a U24 Resource that would allow us to sustain synthesis of phosphorescent probes, making them available to a broad range of biomedical researchers across different fields. Simultaneously, we will generate software for measuring/imaging oxygen by phosphorescence lifetime and will establish a center to provide consultations and training of new users interested in the method. The work will be performed at two closely collaborating sites: the University of Pennsylvania (probe chemistry, software development) and Martinos Center for Biomedical Imaging at the MGH (software development, user training). Our laboratories have long history of productive collaboration as well as multiple collaborations and contacts with researchers interested in oxygen. These collaborations along with the past experience of running a neuroscience research resource will help us to establish an effective program, making the phosphorescence- based oximetry accessible to a broad user base.

磷光淬灭氧气成像的资源 项目概要 组织氧合的实时、微创和空间分辨测量具有 有潜力改变我们对许多临床问题的理解，包括肿瘤生物学的问题， 中风治疗、眼科、组织再生等。多年来我们实验室已 开发了一种用于生物系统中氧气动态成像的微创方法，称为 磷光猝灭血氧定量法。最近，该方法已通过双光子扩展 磷光寿命显微镜 (2PLM)，能够对组织中的氧梯度进行 3D 动态成像 具有微米级分辨率，为神经科学、干细胞生物学、癌症研究开辟新视野 免疫学、组织工程和其他几个领域。甚至最近，结合 切伦科夫激发发光扫描成像 (CELSI) 的磷光断层扫描 接受放射治疗的组织中临床前环境中的 3D 氧气。同时， 基于磷光的血氧测定法因其潜力越来越受到医学界的关注 直接评估创伤组织的生理状态，优化输血效果以及 监测光动力和放射治疗进展的标记。这些和其他应用程序有 为磷光技术在不同生物医学领域的广泛传播奠定了基础。 磷光猝灭方法的核心是特殊的氧探针，经过开发和 我们的实验室不断优化。这些探针的合成，包括最新的和最 有效的探针 Oxyphor 2P 并不简单，标准的商业化途径目前还不可行。 在这里，我们建议建立一个 U24 资源，使我们能够维持磷光的合成 探针，使它们可供不同领域的广泛生物医学研究人员使用。 同时，我们将生成通过磷光寿命测量/成像氧气的软件，并将 建立一个中心，为对该方法感兴趣的新用户提供咨询和培训。这项工作将是 在两个密切合作的地点进行：宾夕法尼亚大学（探针化学、软件 开发）和麻省总医院马蒂诺斯生物医学成像中心（软件开发、用户培训）。 我们的实验室有着悠久的富有成效的合作历史以及多种合作和联系 与对氧气感兴趣的研究人员。这些合作以及过去运营的经验 神经科学研究资源将帮助我们建立有效的计划，使磷光- 基于血氧测定法可供广泛的用户群使用。