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3D Printed Calcium Phosphate Scaffolds with Natural Medicinal Compounds for Dental Applications

用于牙科应用的含天然药用化合物的 3D 打印磷酸钙支架

基本信息

批准号：
10450826
负责人：
SUSMITA BOSE
金额：
$ 34.72万
依托单位：
WASHINGTON STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10450826
关键词：
3D Print Aloe vera plant Alveolar Ridge Augmentation Anti-Inflammatory Agents Arthritis Autologous Autologous Transplantation Beds Biocompatible Materials Biological Biological Availability Biomimetics Bone Diseases Bone Tissue Bone Transplantation Cells Ceramics Chelating Agents Chemistry Chemopreventive Agent Cholecalciferol Clinical Clinical Research Curcumin Data Defect Dental Dental Materials Dentistry Distal Dose Exhibits FASTK Gene Femur Filler Goals Growth Factor Harvest Hydrophobicity Immune response Implant In Vitro Inflammation Kinetics Knowledge Liposomes Measures Mechanics Metals Methods Micelles Modeling Nutrient Operative Surgical Procedures Orthopedics Oryctolagus cuniculus Osseointegration Osteoblasts Osteoclasts Osteogenesis Patients Performance Polyethylene Glycols Polymers Porosity Powder dose form Property Proteins Rattus Research Role Serum Site Spinal Fusion Testing Tissues Titanium Trace Elements Tumeric Weight-Bearing state Work absorption angiogenesis anti-cancer antimicrobial base bone bone cell bone healing bone health bone repair calcium phosphate clinical application clinically relevant design healing improved in vivo innovation maxillofacial mechanical properties metabolic rate next generation osteoclastogenesis osteogenic polycaprolactone practical application preclinical study reconstruction repaired response scaffold

项目摘要

Abstract This revised R01 application aims to use natural medicinal compounds (NMCs) to enhance bioactivity of synthetic bone grafts. The delivery of NMCs in synthetic bone grafts, such as calcium phosphate (CaP) scaffolds, is critical to modulate bone cell-materials interactions to improve in vivo osteogenesis and angiogenesis. Using NMCs, we propose to investigate osteoinductive, 3D-Printed (3DP) CaP bone grafts without using any growth factors (GF) or proteins towards applications in orthopedics and dentistry. However, there still exists a clinical need to innovate osteoinductive synthetic resorbable biomaterials that will promote angiogenesis, exhibiting biological properties similar to autografts. We propose to use NMCs such as curcumin from turmeric, acemannan from aloe vera, and essential nutrients such as Vitamin D3 to improve osseointegration in CaPs instead of using GFs. In our preliminary studies, we have seen promising results showing angiogenesis and enhanced osteogenesis due to the presence of NMCs. We have found that curcumin can enhance osteogenesis and angiogenesis of 3DP CaP scaffolds. From our previous work, we have also established that addition of dopants such as Mg2+, Si4+, Zn2+ and Ag+ in CaP can improve mechanical properties, enhance osteogenesis and angiogenesis while improving antimicrobial response in vivo. Combining our exciting preliminary results on NMCs and established data on dopant chemistry, we propose to demonstrate next generation of synthetic bone grafts without any GF or proteins for applications in dentistry and orthopedics, which is the premise of this application. The objective of this research is to test our central hypothesis that NMC-loaded doped CaP porous 3D Printed (3DP) scaffolds will enhance osteogenic and angiogenic properties in vivo. The rationale is that once we understand the required dose for different NMCs, their release kinetics, and the mechanism of bone cell- materials interactions modulation, the key knowledge gaps, we can design GF free synthetic bone grafts similar to autografts in clinical applications. Our long-range goal is to demonstrate clinically relevant patient matched, osteogenic and angiogenic doped CaP or CaP– polymer scaffolds with NMCs that will substitute autologous bone for repair, replacement, and augmentation in orthopedics and dentistry. To achieve our research objectives, we propose two Specific Aims. Aim 1 is focused on understanding the influence of NMCs on enhanced bioactivity of porous CaP ceramic, and ceramic-polymer composite scaffolds in vitro. Aim 2 is focused on measuring osteogenesis and angiogenesis in vivo using NMC loaded porous CaP ceramic and ceramic-polymer composite scaffolds. If successful, NMCs can also work with other materials for dental and orthopedic applications.

摘要