Resorbable Calcium Phosphate Ceramics for Bone Graft.

说明(申请人提供)：磷酸钙(CaP)基陶瓷因其良好的生物相容性而被用于硬组织工程。有必要开发具有受控降解动力学的可生物降解陶瓷材料，作为支架并支持骨重建。我们的长期目标是阐明帽基材料和支架的强度损失机制，以开发特定应用的骨移植。有关帽基材料受控降解行为的基本信息，以确定最佳材料组成，可以帮助我们根据应用需求设计和定制可吸收组织工程骨置换。这项研究的目的是验证我们的中心假设，即帽基陶瓷中的化学和微观结构可以改善这些材料的强度损失。我们的初步数据表明，微量的痕量元素(掺杂剂)可以对帽子的物理和机械性能产生重大影响。细胞-材料的相互作用也会受到微量元素存在的影响。其具体目的是：1)通过合成、加工、表征和体内外骨细胞与材料的相互作用，研究不同钙磷比(1.25：1、1.33：1和1.5：1)的纳米骨帽的作用。2)研究锌、镁、硅、锶四种杂质氧化物的单元素和多元素组成，以及三种钙磷比分别为1.25：1、1.33：1和1.5：1的帽状陶瓷对体内外吸收的影响。3)利用快速成型技术研制出平均孔径为300微米、孔隙率为30%和60%的三维连通的定制孔隙帽结构，验证孔隙率对其性能的影响，并研究体外和体内的相互作用。为了实现这些目标，我们将进行一系列研究，包括用单元素和多元素掺杂合成纳米帽，表征其化学、物理和力学性能，以及在大鼠和兔模型上的体内外强度损失行为。预计这项研究的结果将导致具有量身定制的降解动力学的CAPS的开发，可用于脊柱融合、颌面和颅面植入以及小规模骨缺损应用。 骨移植用与公共健康相关的可再吸收磷酸钙陶瓷 磷酸钙陶瓷因其良好的生物相容性而被广泛应用于硬组织工程领域。本研究的目的是验证我们的中心假设，即磷酸钙(CaP)基陶瓷中的化学成分和微观结构可以改善这些材料的强度损失。预计这项研究的结果将导致具有量身定制的降解动力学的CAPS的开发，可用于脊柱融合、颌面和颅面植入以及小规模骨缺损应用。

DESCRIPTION (provided by applicant): Calcium phosphate (CaP) based ceramics are used in hard tissue engineering because of their excellent biocompatibility. There is a need for the development of biodegradable ceramic materials with controlled degradation kinetics that will act as a scaffold and support bone remodeling. Our long range goal is to elucidate strength loss mechanism in CaP based material and scaffold to develop bone graft for specific application. Fundamental information on controlled degradation behavior of CaP based materials to identify optimal material composition can help us design and tailor resorbable tissue engineered bone replacement based on application needs. The objective of this research is to test our central hypothesis, which is chemistry and microstructure in CaP based ceramics can modify strength loss in these materials. Our preliminary data indicate that a minimum amount of trace elements (dopants) can have significant effects on physical and mechanical properties of CaPs. Cell-materials interactions can also be influenced by the presence of trace elements. The specific aims are 1) To investigate effects of nanoscale CaP with three different Ca to P ratios, 1.25:1, 1.33:1 and 1.5:1, through synthesis, processing, characterization and in vitro and in vivo bone cell-materials interactions. 2) To determine the effects of four dopants, Zinc, Magnesium, Silicon, and Strontium oxides in single and multi-element composition, along with three CaP ceramics with Ca:P = 1.25:1, 1.33:1 and 1.5:1 on in vitro and in vivo resorption. 3) To develop 3D interconnected tailored porosity CaP structures using rapid prototyping, with an average 300 microns pore size, and, 30 and 60 volume % porosity and verify the influence of porosity on their properties and study in vitro and in vivo interactions. In order to accomplish these aims, we will conduct a series of studies including synthesis of nanoscale CaPs with single and multi element dopants, characterize their chemical, physical and mechanical properties, and in vitro and in vivo strength loss behavior in rat and rabbit models. It is envisioned that results from the proposed study will lead to the development of CaPs with tailored degradation kinetics that can be used in spinal fusion, maxillo- and cranio-facial implants and small scale bone defect applications. PUBLIC HEALTH RELEVANCE Resorbable Calcium Phosphate Ceramics for Bone Graft Calcium phosphate (CaP) based ceramics are used in hard tissue engineering because of their excellent biocompatibility. The objective of this research is to test our central hypothesis, which is chemistry and microstructure in Calcium phosphate (CaP) based ceramics can modify strength loss in these materials. It is envisioned that results from the proposed study will lead to the development of CaPs with tailored degradation kinetics that can be used in spinal fusion, maxillo- and cranio-facial implants and small scale bone defect applications.