Core C: Genomics Core

摘要 U19的基因组学核心将提供最先进的转录组学、小转录组学和外泌体 转录组学分析支持在三个项目中研究的器官和细胞系统：脑（项目1）; 心脏（项目2）;肝脏和骨骼肌（项目3）。所有拟议的方法和分析工具， 这些核心是常规使用和完善的。具体而言，基因组学核心将提供以下内容 分析： RNA分析：编码RNA和长链非编码RNA（lncRNA）的转录组学研究和分析将 对每个项目特定的组织和细胞进行。 microRNA分析：microRNA（miRNAs）和其他小的调控RNA将从组织中分离出来， 三个项目的细胞。此外，外泌体分析将用于确定是否循环 外泌体microRNAs（miRNAs）与三种肿瘤中靶组织和细胞中观察到的变化相关。 项目 基因组学核心还将提供结果的综合分析，以确定转录变异的作用， 包括编码和非编码RNA，在正常衰老（正常生命过程，NLC）中，在宫内生长中的衰老 在狒狒组织或细胞中，限制性后代（IUGR）和妊娠期间母体肥胖（MO）的后代 以及皮质醇替代干预（CRI）狒狒的组织和细胞。核心 还将协助这些项目确定这四个区域中对衰老反应的协调分子网络。 动物群体，并确定调节这些网络的中心枢纽。网络工具将用于整合 多个数据集（例如，miRNA和mRNA）进入高维网络，作为开发系统的第一步 非人灵长类动物（NHP）的衰老分析。 这三个项目都将使用基因组学核心的服务。我们的协同方法分析样品 对所有三个项目使用相同的方法，可以对样品进行多重分析， 成本和减少分析工件。此外，这种方法允许为所有人整合“组学”数据， 组织分析，这将提供一个在“系统”的反应，在五个狒狒队列老化的第一眼。

ABSTRACT The Genomics Core of the U19 will provide state-of-the-art transcriptomics, small transcriptomics and exosome transcriptomics analysis support for organ and cell systems investigated in the three projects: brain (Project 1); heart (Project 2); and liver and skeletal muscle (Project 3). All methodologies and analysis tools proposed for the Core are routinely used and well-established. Specifically, the Genomics Core will provide the following analyses: RNA Analysis: Transcriptomic studies and analyses of coding RNAs and long noncoding RNAs (lncRNA) will be performed on tissues and cells specific to each project. microRNA Analysis: microRNAs (miRNAs) and other small regulatory RNAs will be isolated from tissues and cells from the three projects. In addition, exosome analysis will be used to determine whether circulating exosome microRNAs (miRNAs) correlate with changes observed in target tissues and cells in the three Projects. The Genomics Core will also provide integrated analysis of results to establish the role of transcript variation, including coding and non-coding RNAs, in normal aging (normal life course, NLC), aging in intrauterine growth restricted offspring (IUGR) and offspring of maternal obesity during pregnancy (MO) in baboon tissues or cells for each Project, as well as for tissues and cells in corticol replacement intervention (CRI) baboons. The Core will also assist the Projects in identifying coordinated molecular networks responsive to aging in these four animal cohorts, and identify central hubs regulating these networks. Network tools will be used to integrate multiple datasets (e.g., miRNA and mRNA) into high-dimensional networks as a first step to develop a systems analysis of the aging nonhuman primate (NHP). All three projects will use the services of the Genomics Core. Our synergistic approach analyzing samples using the same method for all three projects allows multiplexing of samples for analysis, minimizing reagent costs and reducing analysis artifacts. In addition, this approach allows for integration of “omics” data for the all tissues analyzed, which will provide a first look at the “systems” response to aging in the five baboon cohorts.